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Application of beauverin derived from marine fungi in marine fouling organism control

A technology of beauveria and marine fouling, applied in biocide-containing paints, antifouling/underwater coatings, coatings, etc., to achieve the prevention and control of marine fouling organisms, reliable and stable sources, and great potential for popularization and application Effect

Active Publication Date: 2020-01-10
SUN YAT SEN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, in the field of marine antifouling, there is no relevant report disclosing the role of beauveria in biofouling control

Method used

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  • Application of beauverin derived from marine fungi in marine fouling organism control
  • Application of beauverin derived from marine fungi in marine fouling organism control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The beauveria of the present invention can be isolated from the thallus of mangrove-derived fungi. Specific steps are as follows:

[0022] Use rice medium to ferment and cultivate the endophytic fungi of mangrove forests, and culture them statically for 28 days. The fermented product is soaked in ethyl acetate. The crude extract was separated by silica gel column chromatography, and the beauveriacin compound represented by the above formula was obtained in the isocratic eluent of petroleum ether / ethyl acetate (volume ratio: 7 / 3).

Embodiment 2

[0024] Anti-biofouling activity experiment is carried out to the beauveria in embodiment 1:

[0025] In the experimental group, the beauverum compound was dissolved in methanol to prepare a solution with a concentration of 282.6 μg / mL. Add 1 mL of this solution to a petri dish with a diameter of 6 cm, and make it evenly cover the bottom of the petri dish. After the solvent is completely volatilized, the content of beauvericin coated on the bottom of the petri dish is 10 μg / cm 2 .

[0026] Add 1mL of methanol to the control group, so that the solution is evenly distributed on the bottom of the petri dish, and then add 10mL of seawater after the solvent is completely evaporated.

[0027] Only 10 mL of seawater was added to the blank group.

[0028] Three parallel samples were set up for each of the experimental group, the control group and the blank group. Add 30 netted barnacle Venus larvae to each sample. Place them in a constant temperature incubator at a temperature of ...

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Abstract

The invention discloses an application of beauverin derived from marine fungi in marine fouling organism control. The application of the beauverin in marine fouling organism control is disclosed for the first time, and the beauverin has a remarkable inhibition effect on adhesion of the marine fouling organisms on solids, and has a remarkable effect under a low-content coating condition. The beauverin can inhibit adhesion of the marine organisms without killing marine organisms, and has a repelling effect. The beauverin is extracted from fermented substances of marine fungi, has a reliable andstable source, and has a good application prospect in control of fouling organisms of various marine artificial facilities.

Description

technical field [0001] The invention belongs to the field of marine antifouling coatings, and in particular relates to the application of beauvericin derived from marine fungi in biological control of marine fouling. Background technique [0002] In the historical process of human development and utilization of the ocean, biofouling has always been one of the major problems that need to be solved urgently in industries such as marine transportation, marine engineering, and marine fishery. Marine biofouling refers to the phenomenon that ships immersed in seawater or various artificial facilities are attached by marine microorganisms, plants or animals, which adversely affects human economic activities. The main harm is to increase the roughness and weight of the ship, thereby increasing Large resistance, reducing speed, increasing fuel consumption and hull maintenance costs; hindering the normal work of various marine facilities, causing drift, imbalance and even capsizing; i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09D5/16
CPCC09D5/1625
Inventor 佘志刚蔡润林严涛曹文浩
Owner SUN YAT SEN UNIV
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