Formula of probiotics for inhibiting pathogenic bacteria of colorectal cancer and screening method thereof

Abstract

The invention discloses a formula of probiotics for inhibiting pathogenic bacteria of colorectal cancer and a screening method thereof. The screening method of the probiotics comprises the following steps: finding the pathogenic bacteria of the colorectal cancer according to metagenome data and abundance of intestinal flora of normal people and colorectal cancer patients, then constructing an intestinal flora interaction network to screen the probiotics capable of inhibiting the pathogenic bacteria of the colorectal cancer, adjusting the proportion of the probiotics, and finally verifying. Theformula of the probiotics obtained by the method is shown in the specification, wherein the content of the clostridium butyricum is 1.3*10 <10> CFU / ml; the content of enterococcus faecalis is 1*10 <9> CFU / ml, the content of lactobacillus brevis is 1*10<9> CFU / ml, the content of lactobacillus plantarum is 1*10 <9> CFU / ml, the content of lactobacillus rhamnosus is 1*10 <9> CFU / ml, the content of lactobacillus sake is 1*10 <9> CFU / ml and the content of leuconostoc mesenteroides is 1*10 <9> CFU / ml. The method disclosed by the invention has the advantages that (1) an intestinal flora interaction network is constructed, so that the screened probiotic formula can be better colonized in intestinal tracts; (2) the obtained probiotic formula does not generate drug resistance, and is safe, effectiveand excellent in performance; and (3) an online warehouse database of human intestinal microbiome is constructed, so that the result is more universal and reliable.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a probiotic formula for inhibiting colorectal cancer pathogenic bacteria and a screening method thereof. Background technique [0002] Colorectal cancer (CRC) is a common malignant tumor in humans and is currently listed as the third most common cancer in the world. With the improvement of people's living standard and the change of diet structure, its morbidity and mortality are on the rise, seriously threatening human health. A large number of studies have reported that CRC is associated with various factors, such as obesity, diabetes, sedentary lifestyle, high-fat diet, smoking, alcoholism, age, gender, family history, etc., but its exact pathogenesis is still unclear. [0003] More and more studies this year have suggested that gut microbiota is a very important factor related to the progression of CRC. Gut flora, as an organoid, has a deep interaction relationship with ...

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Problems solved by technology

[0006] Chinese patent CN107937504A discloses a screening method for feeding probiotics resistant to pig E. coli infection, comprising the following steps: screening of piglets infected with E. coli and disease-resistant piglets and collection of intestinal content samples; High-throughput sequencing of intestinal microbiota of piglets and disease-resistant piglets; comparative analysis of intestinal microbial metagenomics of piglets with E. coli infection and disease-resistant piglets; and screening and identification of probiotics against E. coli infection. There are very few samples of anti-colorectal cancer pathogenic bacteria collected, and this method cannot be used to screen probiotics against colorectal cancer pathogens. At the same time, this method does not screen probiotics from the ecological perspective of the intestinal flora , so the screened probiotic formula may have poor colonization ability in the intestinal tract

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