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Method using laccase catalysis to prepare polyaniline

A technology for catalytic preparation and polyaniline, which is applied in the field of enzyme catalysis and can solve problems such as undisclosed sources of laccase

Pending Publication Date: 2020-01-17
CHANGSHU JINDA TECH CO LTD
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0003] Enzyme catalysis is milder than chemical synthesis conditions. Nanjing University of Science and Technology CN105244510B discloses a method of electropolymerizing aniline by constant potential method while laccase is catalyzed, but the source of laccase is not disclosed in this patent

Method used

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  • Method using laccase catalysis to prepare polyaniline
  • Method using laccase catalysis to prepare polyaniline

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Experimental program
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Embodiment

[0015] Laccases from different fungal species were used to catalyze the polymerization of aniline, and a 5ml centrifuge tube was used as a reactor, such as figure 1 as shown,

[0016] Add aniline, sodium dodecylbenzenesulfonate, and 0.4ml of crude laccase solution from different sources to the centrifuge tube, make up to 4ml with purified water, and then adjust the pH of the system to 3.0 by adding acetic acid buffer to form a reaction system, and let it stand at room temperature After 24 hours of reaction, it is obtained; the concentration of aniline in the reaction system is 10mmol / L, and the concentration of sodium dodecylbenzenesulfonate (SDBS) is 20mmo / L.

[0017] figure 1 From left to right in the middle are the crude enzyme solutions derived from Pleurotus eryngii, Coprinus comatus, AS bacteria, AC bacteria, Russula, Ganoderma lucidum, and Pleurotus. The results show that Ganoderma lucidum turns dark green after the reaction, and the color is the darkest, proving that ...

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Abstract

The invention belongs to the technical field of enzyme catalysis and particularly relates to a method using laccase catalysis to prepare polyaniline. The method includes: adding aniline, sodium dodecyl benzene sulfonate and sweet ganoderma lucidum crude enzyme liquid containing laccase into an aqueous solution, adding an acetic acid buffer solution to regulate system pH to 3.0 to form a reaction system, and performing standing reaction at room temperature to obtain the polyaniline. The method has the advantages that laccase from different kinds of fungi is screened, the fact that the sweet ganoderma lucidum crude enzyme liquid containing laccase can efficiently catalyze aniline polymerization to prepare the polyaniline is discovered, and the catalytic activity of the laccase from the sweetganoderma lucidum crude enzyme liquid is evidently higher than that of the laccase from pleurotus eryngii, coprinus comatus, AS fungi, AC fungi, Russula vinosa Lindblad or oyster mushrooms.

Description

technical field [0001] The invention belongs to the technical field of enzyme catalysis, and more specifically relates to a method for preparing polyaniline catalyzed by laccase. Background technique [0002] Polyaniline is a conductive polymer material, which is used in electronic products, luminescent materials, antiseptics, antistatic and so on. Polyaniline is obtained through the oxidative polymerization reaction in this case. Usually, an oxidizing agent is added to the acidic aqueous solution of aniline to react to obtain a polyaniline precipitate, which is then filtered, washed, dried and ground to obtain a polyaniline product. [0003] Enzyme catalysis is milder than chemical synthesis conditions. Nanjing University of Science and Technology CN105244510B discloses a method for electropolymerizing aniline by potentiostatic method while laccase catalysis is used, but the source of laccase is not disclosed in this patent. The source of laccase used in the synthesis of p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/00
CPCC12P13/001
Inventor 杨智钧王锦锦刘丹丹吕爱平
Owner CHANGSHU JINDA TECH CO LTD
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