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Kit for detecting typhoid and paratyphoid and application thereof

A paratyphoid and kit technology, applied in the field of medical testing, can solve the problems of a large number of test tubes, cumbersome operations, large sample volume of testing reagents, etc., and achieve the effect of improving efficiency

Pending Publication Date: 2020-01-03
广州达安临床检验中心有限公司 +4
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Veda reaction and Waifei reaction are traditional methods for detecting typhoid and paratyphoid fever, but the traditional detection methods of Vita reaction and Waifei reaction are mainly test tube agglutination method, which mainly has the following problems: 1. The operation is cumbersome , a large number of test tubes are required, and the amount of detection reagent required is large
2. It is difficult to judge the results clearly with the naked eye after incubation in a water bath box, and it is not easy to judge whether the agglutination reaction has completely occurred

Method used

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  • Kit for detecting typhoid and paratyphoid and application thereof
  • Kit for detecting typhoid and paratyphoid and application thereof
  • Kit for detecting typhoid and paratyphoid and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0068] The present embodiment provides a test kit for detecting typhoid and paratyphoid, the composition of which includes:

[0069]

[0070] Wherein, the Widal antigen and the Waffen antigen are stored in a refrigerator at 2-8°C, taken out before use, and used after being equilibrated to room temperature (15-28°C). When used, they were diluted 10 times with 0.9% sterile normal saline to contain bacteria 7.0×10 8 bacteria / mL bacterial suspension, and mixed with sterile safflower solution at a ratio of 1:10 before use. The preparation method is ready-to-use.

Embodiment 2

[0072] This embodiment provides the detection method of the kit described in Embodiment 1, specifically as follows:

[0073] 1. Prepare

[0074] Take out the reagents (Salmonella typhi O, Salmonella typhi H, Salmonella paratyphi A, Salmonella paratyphi B, Salmonella paratyphi C, Proteus OX19, Proteus OX2, Proteus OXK) kept in the refrigerator, and balance to Room temperature (15-28°C).

[0075] Dilute the above reagent 10 times with 0.9% sterile saline to contain bacteria 7.0×10 8 bacteria / mL, and then add 10 times the volume of sterile safflower solution. Wherein, the concentration of the sterile safflower solution is: 0.5%.

[0076] 2. Detection

[0077] 1) Serum samples to be tested, 8 positive serum samples (Salmonella typhi O positive serum, Salmonella typhi H positive serum, Salmonella paratyphi A positive serum, Salmonella paratyphi B positive serum, Salmonella paratyphi C positive serum Positive serum, Proteus OX19-positive serum, Proteus OX2-positive serum, Prote...

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Abstract

The invention provides a kit for detecting typhoid and paratyphoid. The kit comprises a micro-reaction plate with a V-shaped bottom, a safranine solution, Widal antigens and Weil Felix antigens. The Widal antigens comprise salmonella typhi O, salmonella typhi H, salmonella paratyphi A, salmonella paratyphi B and salmonella paratyphi C. The Weil Felix antigens comprise proteus OX19, proteus OX2 andproteus OXK. In a detection process, agglutination formed by a positive serum sample and the Widal antigens or the Weil Felix antigens at a hole bottom of the micro-reaction plate fits with a color development effect of the safranine solution, and the Widal antigens and the Weil Felix antigens, judgment of a detection result for the typhoid and the paratyphoid is relatively clear and accurate.

Description

technical field [0001] The invention belongs to the field of medical testing, in particular to a kit for detecting typhoid and paratyphoid and its application. Background technique [0002] Typhoid fever is an intestinal infectious disease caused by the typhoid serotype of Salmonella enterica subspecies enterica, and paratyphoid is a disease similar to typhoid fever caused by the paratyphoid A, B or C serotypes of Salmonella enterica subspecies enterica. The symptoms of paratyphoid A and B are similar to those of typhoid fever, but generally the disease is milder, the course of the disease is shorter, and the fatality rate is lower; the symptoms of paratyphoid fever C are quite different, and can be manifested as mild typhoid fever, acute gastroenteritis or sepsis. Outbreaks of typhoid and paratyphoid can occur due to water and food contamination. [0003] Veda reaction and Waifei reaction are traditional methods for detecting typhoid and paratyphoid fever, but the traditio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569
CPCG01N33/56916G01N33/56911Y02A50/30
Inventor 张小龙
Owner 广州达安临床检验中心有限公司
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