Root exudate collection and detection methods

A technology of root exudates and collection tank, applied in the field of agricultural cultivation, can solve the problems of complex production operation, high growth cost, lack of experimental basis and the like

Inactive Publication Date: 2019-12-31
SHANDONG AGRI SUSTAINABLE DEV INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Soil disinfection, reasonable crop rotation and intercropping systems, and grafting are mainly used to solve the obstacles to continuous cropping, but the production operation is complicated and the growth cost is high
Some researchers believe that using atomized nozzles to continuously rinse the root system in aeroponics may partially or completely alleviate the autotoxicity of tomato plants, but due to the low content of root exudates, there is no strong experimental basis, and there is an urgent need for a Root exudate collection and detection method to verify the theory

Method used

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  • Root exudate collection and detection methods
  • Root exudate collection and detection methods

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1 provides a kind of root system exudate collection system, such as figure 1 As shown, it includes a liquid supply pool 1, a filtration system, a cultivation tank 17, and a collection system; the liquid supply pool 1, the filtration system, the cultivation tank 17, and the collection system are connected by a liquid supply system 18; the liquid supply pool 1 The internal storage nutrient solution is supplied to the cultivation tank 17 by the water pump 2; the inside of the cultivation tank 17 is provided with a liquid supply pipeline; two atomizing nozzles 14 are arranged on the said liquid supply pipeline;

[0060] Said water pump 2 monitors the liquid supply volume by a water pump pressure gauge 3 .

[0061] The filter system includes a primary filter 5 and a secondary filter 6 .

[0062] The reflux nutrient solution of the filter system flows back to the liquid supply tank 1 through the filter reflux ball valve 4 .

[0063] The filtered nutrient solution...

Embodiment 2

[0069] Embodiment 2 provides a kind of root system exudate collection system, such as figure 2 As shown, it includes a liquid supply pool 1, a filtration system, a cultivation tank 17, and a collection system; the liquid supply pool 1, the filtration system, the cultivation tank 17, and the collection system are connected by a liquid supply system 18; the liquid supply pool 1 The internal storage nutrient solution is supplied to the cultivation tank 17 by the water pump 2; the inside of the cultivation tank 17 is provided with a liquid supply pipeline; the liquid supply pipeline is provided with two atomizing nozzles 14;

[0070] Said water pump 2 monitors the liquid supply volume by a water pump pressure gauge 3 .

[0071] The filter system includes a primary filter 5 and a secondary filter 6 .

[0072] The reflux nutrient solution of the filter system flows back to the liquid supply tank 1 through the filter reflux ball valve 4 .

[0073] The filtered nutrient solution of...

Embodiment 3

[0079] Embodiment 3 provides a kind of root exudate collecting method, it uses the root exudate collection system in embodiment 2 to collect root exudate, may further comprise the steps:

[0080] a. Provide nutrient solution: add nutrient solution to the liquid supply tank 1, turn on the water pump 2 to supply the nutrient solution to the cultivation tank 17, control the spray speed by the spray solenoid valve 12, spray the liquid for 50 seconds every 5 minutes, and spray the spray nozzle 14 The flow rate is 1 liter per minute;

[0081] b. Collect exudate: take off the chromatographic column 19 that Amberlite XAD-4 resin is housed, after the root exudate that is absorbed in the chromatographic column 19 is eluted with 100ml methanol, with vacuum freeze drier (model MAXI dry Lyo) The eluate was concentrated and made to volume with 1 ml of methanol, and finally filtered through a 0.45 μm filter membrane to obtain root exudates.

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Abstract

The invention discloses a root exudate collection system, comprising a liquid supply pool, a filtering system, a cultivation tank and a collection tank which are connected through a liquid supply system. Nutrient solution is stored in the liquid supply pool and supplied to the cultivation tank by a water pump. A liquid supply pipeline is arranged in the cultivation tank. Atomizing nozzles and a chromatographic column are arranged on the liquid supply pipeline. The invention further discloses a collection method using the root exudate collection system and a detection method for collected rootexudate. The collection and detection methods can be used to collect and detect root exudate in real time. The influence of other factors on the growth of crops is eliminated. The effect of root exudate on the growth of plants is judged by observing the growth condition of the crops in the cultivation tank, and the effect of relieving the autotoxicity of the crops by leaching the roots is verified.

Description

technical field [0001] The invention relates to the technical field of agricultural cultivation, in particular to a method for collecting and detecting root exudates. Background technique [0002] The obstacle of continuous cropping has become an important factor restricting the sustainable development of vegetable production in some areas. Its occurrence is not only related to soil infectious diseases and deterioration of soil physical and chemical properties, but also to the autotoxic effect caused by root exudates and residue decomposition products. related. Root secretion is an important source of allelochemicals produced by plants. There are four release pathways for allelochemicals: overflow from the plant surface, be washed by rain to the lower plants or infiltrate into the soil to show allelopathic effects; Spillage directly produces allelopathic effects; some volatile allelochemicals volatilize into the environment through plant surfaces; plant residues or litter d...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/02
CPCG01N30/02G01N30/06
Inventor 赵旭刘涛
Owner SHANDONG AGRI SUSTAINABLE DEV INST
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