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lncRNA coding gene related to sheep skeletal muscle development and its application

A technology that encodes genes and skeletal muscles, applied in the field of biology, can solve problems such as functional research is still in its infancy.

Active Publication Date: 2021-02-02
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, some progress has been made in the identification of lncRNA in the development of livestock and poultry skeletal muscle, but its functional research is still in the initial stage (Dai Menghong et al., 2016; Yang Bing et al., 2018; Zhou Rui et al., 2018)

Method used

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  • lncRNA coding gene related to sheep skeletal muscle development and its application
  • lncRNA coding gene related to sheep skeletal muscle development and its application
  • lncRNA coding gene related to sheep skeletal muscle development and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0071] Example 2 Effect of lncRNA TCONS_00544451 on Proliferation and Differentiation of Sheep Skeletal Muscle Satellite Cells

[0072] 1. Isolation, culture and induced differentiation of skeletal muscle satellite cells from Duolang sheep. The growth medium of sheep skeletal muscle SCs is formulated with DMEM / F12 medium containing 10% horse serum and 20% fetal bovine serum, and the medium for inducing myogenic differentiation is DMEM / F12 medium containing 2% horse serum formulated. The separation and purification of sheep skeletal muscle SCs adopts two-step enzyme digestion method and differential adhesion method, and the steps are as follows:

[0073] (1) Carefully cut open the uterus wrapped with the sheep fetus with ophthalmic scissors, remove the leg muscles of the fetus and soak in PBS containing 1% double antibody (1% penicillin-streptomycin).

[0074] (2) Separate the muscle in a sterilized ultra-clean workbench, remove blood vessels, fibers, sarcolemma and other tis...

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Abstract

The invention provides a lncRNA (long noncoding RNA) encoding gene sheep related to skeletal muscle development as well as application thereof. The lncRNA related to different development stages of Dorang sheep skeletal muscle is identified and analyzed by transcriptome sequencing, the function of the lncRNA is research in the cell level, and the lncRNA which obviously influences the skeletal muscle development is screened. The invention firstly discovers that overexpression of lncRNA TCONS 00544451 has the functions of inhibiting skeletal muscle satellite cell proliferation and promoting cellmyogenic differentiation, and scientific basis and technical support are provided for molecular breeding and variety improvement for improving sheep meat producing performance.

Description

technical field [0001] The invention relates to the technical field of biology, in particular to an lncRNA coding gene related to sheep skeletal muscle development and application thereof. Background technique [0002] China is the world's largest producer and consumer of mutton. However, compared with New Zealand, Australia and other developed countries in the world, the small amount of meat produced by mutton sheep in my country is one of the important factors restricting the production of mutton sheep. The growth and development of sheep skeletal muscle determines the formation of high-yield and high-quality traits in mutton sheep. An in-depth analysis of the molecular mechanism of skeletal muscle growth and development is of great significance for improving the meat production performance of sheep in my country. [0003] Skeletal muscle satellite cells are a kind of muscle-derived stem cells, which are responsible for the regeneration of animal skeletal muscle after inj...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12N5/10C12N5/077
CPCC12N5/0658C12N15/113C12N2310/10C12N2506/1323
Inventor 赵倩君马月辉鲍晶晶薛翔澜关伟军浦亚斌丁洋洋
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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