Bacillus cereus MA23, inoculant and preparation method and application of inoculant

A technology of Bacillus cereus and microbial agent, which is applied in the field of agricultural microorganisms, can solve the problems of pathogenic bacteria developing drug resistance, easily polluting the environment, and reducing the control effect, and achieve the effects of reducing the loss of agricultural products, being environmentally friendly, and low cost

Active Publication Date: 2019-12-27
MICROBIOLOGY INST OF SHAANXI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, long-term use of chemical pesticides will easily pollute the environment, degrade soil quality, cause drug resistanc...

Method used

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  • Bacillus cereus MA23, inoculant and preparation method and application of inoculant
  • Bacillus cereus MA23, inoculant and preparation method and application of inoculant
  • Bacillus cereus MA23, inoculant and preparation method and application of inoculant

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preparation example Construction

[0039] The preparation method of above-mentioned microbial bacterial agent, concrete steps are as follows:

[0040] Step 1, strain activation:

[0041] Inoculate the Bacillus cereus MA23 stored at low temperature on the nutrient agar plate medium for activation culture, and cultivate it at 28-32°C for 20-24 hours; then pick a single colony and inoculate it on the nutrient agar slant medium for expansion culture , cultivated at 28-32°C for 20-24 hours; finally, the bacteria were eluted with 10mL sterile water, and the obtained eluate was used as the inoculum for seed cultivation;

[0042] Step 2, the preparation of seed solution:

[0043] Inoculate the inoculum obtained in step 1 into the seed medium, the volume ratio of the used seed medium to the inoculum is 1:0.5% to 1:5%, at 28~32°C and the initial pH value is 5.5~7.5 Shake culture for 14-20 hours to obtain seed solution;

[0044] Among them, the components and mass percentages of the seed medium used are: 1.0% to 2.0% o...

Embodiment 1

[0077] The preparation method of MA23 microbial bacterial agent, concrete steps are as follows:

[0078] Step 1, strain activation: Inoculate Bacillus cereus MA23 preserved at low temperature on the nutrient agar plate medium for activation culture, and cultivate it at 30°C for 20 hours; then pick a single colony and inoculate it on the nutrient agar slant medium for expansion Intensive culture, cultivated at 30°C for 20 hours; then eluted the bacteria with sterile water, and the obtained eluate was used as the inoculum for seed culture.

[0079] In this example, the Bacillus cereus strain MA23 was stored at -80°C.

[0080] The pH value of the nutrient agar plate medium and the nutrient agar slant medium is 7.0-7.4, and the components and their mass percentages are: peptone 1.0%, beef extract 0.5%, NaCl 0.5%, agar 2.0%, and the balance is Water, the preparation method can refer to the national standard GB / T 4789.28-2003 for preparation.

[0081] Step 2, preparation of seed l...

Embodiment 2

[0087] The preparation method of microbial bacterial agent, concrete steps are as follows:

[0088] Step 1, strain activation:

[0089] Inoculate the low-temperature-preserved Bacillus cereus strain MA23 on the nutrient agar plate medium for activation culture, and cultivate it at 30°C for 24h; Cultivate under low temperature for 20 hours; then elute the bacteria with sterile water, and the obtained eluate is used as the inoculum for seed culture.

[0090] In this example, the Bacillus cereus strain MA23 was stored at -80°C.

[0091] The pH value of the nutrient agar plate medium and the nutrient agar slant medium is 7.0-7.4, and the components and their mass percentages are: peptone 1.0%, beef extract 0.5%, NaCl 0.5%, agar 2.0%, and the balance is water , the preparation method can be prepared with reference to the national standard GB / T 4789.28-2003.

[0092] Step 2, the preparation of seed solution:

[0093] Put 100mL of seed culture medium into 250mL Erlenmeyer flasks,...

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Abstract

The invention discloses a bacillus cereus bacterial strain MA23. The bacillus cereus bacterial strain MA23 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and thepreservation number is CGMCC No.16015. The invention further discloses an inoculant prepared from the MA23 bacterial strain and the preparation and application of the inoculant. The bacillus cereus MA23 can be used for preventing and treating agricultural plant diseases and has efficient and broad-spectrum bactericidal activity.

Description

technical field [0001] The invention belongs to the technical field of agricultural microbes, and relates to a Bacillus cereus MA23, and the invention also relates to a bacterial agent of the above-mentioned Bacillus cereus MA23, and a method for preparing the above-mentioned bacterial agent of the Bacillus cereus MA23 , The present invention also relates to the application of the bacterial agent of the above-mentioned Bacillus cereus MA23. Background technique [0002] Plant disease refers to the process of a series of pathological changes in the physiology, cell and tissue structure of the plant due to the invasion of parasites or the adverse environment during its life, resulting in abnormal external morphology, resulting in reduced yield, poor quality or ecological damage. phenomenon of environmental destruction. At present, infective plant diseases in agricultural production mainly include: bacterial diseases, fungal diseases, viral diseases and nematode diseases. Amo...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P1/00A01P3/00C12R1/085
CPCC12N1/20C12R2001/085C12N1/205
Inventor 马瑜朱海云柯杨李勃
Owner MICROBIOLOGY INST OF SHAANXI
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