Taraxacum kok-saghyz germplasm resource in-vitro preservation method

A technology of in vitro preservation and rubber grass, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as large workload, achieve small workload, reduce succession work, and ensure goodness and purity Effect

Pending Publication Date: 2019-12-24
HEILONGJIANG ACAD OF SCI INST OF NATURAL RESOURCES
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  • Description
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  • Application Information

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Benefits of technology

This patented technology describes methods used during embryonic research to maintain or improve their quality through reducing certain substances called glucosinolate (GI). These techniques include culturing cells containing these compounds into different types of media like liquid nitrogen) which allows them to grow naturally without being affected by external factors such as lightning rain or other environmental changes over several days's horizon. By controllably increasing GI levels within specific ranges, this technique helps keep plants alive longer than usual while also improving resistance against disease attacks. Overall, this new approach provides technical means to enhance natural rubber bacterial resource management capabilities.

Problems solved by technology

This patented technical problem addressed in this patents relates to developing methods for efficiently conserving rubbergrass germplasms during cultivation without causing contaminants such as bacteria, fungi, insects, etc., while ensuring stable propagules from parental generations into commercial products like synthetic rubbers.

Method used

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  • Taraxacum kok-saghyz germplasm resource in-vitro preservation method

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Embodiment 1

[0074] The invention provides a method for in vitro preservation of rubber grass germplasm resources, the steps of which are:

[0075] (1) Preparation of culture medium

[0076] A. Induction medium: MS+6-BA1.5~2.0mg / L+NAA0.1~0.2mg / L;

[0077] B. Differentiation medium: MS+6-BA0.5~1.0mg / L+NAA0.05~0.1mg / L+GA 3 0.1~0.2mg / L;

[0078] C. Proliferation medium: MS+6-BA1.0mg / L+NAA0.1mg / L;

[0079] D. Rooting medium: 1 / 2MS+NAA0.3mg / L;

[0080] E. Germplasm preservation medium: 1 / 2MS+NAA0.3mg / L+mannitol 30mg / L+abscisic acid 1.0mg / L;

[0081] Among them, 30g / L sucrose and 5.5g / L agar powder were added to the medium at each stage, and the pH value was 5.8-6.0;

[0082] (2) Materials and inoculation

[0083] The material is the young leaves of rubber grass. After washing with detergent solution, rinse with running water for 2-4 hours, first disinfect with 75% (volume percentage) alcohol on the ultra-clean workbench for 10 seconds, and then use 0.1% (mass percentage) )HgCL 2 The sol...

Embodiment 2

[0091] The invention provides a method for in vitro preservation of rubber grass germplasm resources, the steps of which are:

[0092] (1) Preparation of culture medium

[0093] A. Induction medium: MS+6-BA1.5~2.0mg / L+NAA0.1~0.2mg / L;

[0094] B. Differentiation medium: MS+6-BA0.5~1.0mg / L+NAA0.05~0.1mg / L+GA 3 0.1~0.2mg / L;

[0095] C. Proliferation medium: MS+6-BA1.0mg / L+NAA0.1mg / L;

[0096] D. Rooting medium: 1 / 2MS+NAA0.3mg / L;

[0097] E. Germplasm preservation medium: 1 / 2MS+NAA0.3mg / L+mannitol 30mg / L+abscisic acid 2.0mg / L;

[0098] Among them, 30g / L sucrose and 5.5g / L agar powder were added to the medium at each stage, and the pH value was 5.8-6.0;

[0099] (2) Materials and inoculation

[0100] The material is the young leaves of rubber grass. The material is the young leaves of rubber grass. After washing with detergent solution, rinse with running water for 2-4 hours, first disinfect with 75% (volume percentage) alcohol on the ultra-clean workbench for 10 seconds, an...

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Abstract

The invention belongs to the technical field of plant germplasm resource preservation and tissue culture and particularly relates to a taraxacum kok-saghyz germplasm resource in-vitro preservation method. The method includes: (1) culture medium preparation; (2) material preparation and inoculation; (3) callus induced differentiation; (4) adventitious bud multiplication; (5) rooting culture; (6) germplasm preservation culture; (7) recovery culture. A germplasm preservation culture medium made according to a special formula is adopted, MS concentration is reduced to 1/2MS, and mannitol and abscisic acid are added. Compared with a conventional tissue culture method, the method has advantages that the subculture period of taraxacum kok-saghyz test-tube plantlets is prolonged from 2 months to 10-12 months, growth limited preservation of the test-tube plantlets is realized, the survival rate is still 95% after the test-tube plantlets are preserved for 10 months, normality in growth of plantsis realized, and the test-tube plantlets on a normal culture medium only survives for 2 months.

Description

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Claims

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Application Information

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Owner HEILONGJIANG ACAD OF SCI INST OF NATURAL RESOURCES
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