Major QTL site for flowering time-related traits of brassica napus, SNP molecular markers and application
A Brassica napus, molecular marker technology, applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc. The effect of large-scale promotion of application and accelerated process
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Embodiment 1
[0030] The mensuration of the phenotype of embodiment 1 Brassica napus anthesis trait
[0031] 1. Determination of Anthesis Phenotypes in Associated Populations
[0032] (1) A natural population of 324 high-generation lines of Brassica napus from all over the world was formed, and a 3-year and 2-point investigation of flowering period traits was completed at the Yangluo Experimental Base of Wuhan City and the Experimental Base of Yangzhou Academy of Agricultural Sciences.
[0033] (2) The seedlings were fixed by direct seeding, the row spacing was 33 cm, the plant spacing was 15 cm, 4 rows in each plot, and three replicates were randomly designed. Protective rows were planted around the test material fields.
[0034] (3) Flowering period: the number of days obtained by subtracting the planting date from the date when 25% of the plants in the whole area begin to flower.
[0035] (4) Use the BLUP method (http: / / www.extension.org / pages / 61006) to integrate multi-year multi-point...
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