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Application of TNFSF11 (tumor necrosis factor superfamily member 11) gene rs9525641 SNP (single nucleotide polymorphism)

A gene and genome technology, applied in the field of biomedicine, can solve problems such as unresearched, contradictory, and not necessarily in line with reasoning.

Active Publication Date: 2019-12-20
YIXING PEOPLES HOSPITAL +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010]TNFSF11 / TNFRSF11A plays an important role in host immune regulation, but when HCV invades the body, whether the genetic polymorphism of TNFSF11 / TNFRSF11A will affect the outcome of HCV infection, currently No one has studied it at home and abroad, and there is no research report on the correlation between SNPs of TNFSF11 or TNFRSF11A and HCV infection at home and abroad
[0011] In epidemiological research, the determination of disease risk factors and correlation research can only be obtained through rigorous epidemiological design and research, and rigorous data analysis. All kinds of reasoning are usually the results and conclusions that are in line with scientific research hypotheses. However, the verified results may not conform to the reasoning, and sometimes even contradict

Method used

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  • Application of TNFSF11 (tumor necrosis factor superfamily member 11) gene rs9525641 SNP (single nucleotide polymorphism)
  • Application of TNFSF11 (tumor necrosis factor superfamily member 11) gene rs9525641 SNP (single nucleotide polymorphism)
  • Application of TNFSF11 (tumor necrosis factor superfamily member 11) gene rs9525641 SNP (single nucleotide polymorphism)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Example 1 Preparation of substances for detecting human TNFSF11 gene rs9525641 SNP / allele / genotype

[0064] The method for preparing the material for detecting or screening the chronicity of hepatitis C in Chinese Han population for detecting human TNFSF11 gene rs9525641SNP / allele / genotype comprises the following steps:

[0065] S1: Extraction of Genomic DNA

[0066] (1) Purple hat vacuum blood collection tubes were used to collect peripheral venous blood from the research subjects, absorb whole blood, add an equal volume of phosphate buffer, and mix well; slowly add lymphocyte separation solution, centrifuge at a certain speed for a certain period of time, absorb white blood cells, and use phosphoric acid Wash with buffer solution, centrifuge to discard supernatant, and freeze;

[0067] (2) Leukocytes were resuspended with an equal volume of phosphate buffer, and then DNA was extracted with a commercial QIAGEN DNA extraction kit to obtain a DNA solution, which was fro...

Embodiment 2

[0102] Example 2 Application of rs9525641 SNP / allele / genotype of TNFSF11 gene

[0103] The method for detecting the SNF typing of the rs9525641 single nucleotide polymorphism or genotype of the TNFSF11 gene in the human genome comprises the following steps: dispensing the material for detecting the rs9525641 SNP or genotype of the TNFSF11 gene in the human genome into sterile PCR tube; take the system solution and add it to a 384-well plate, then add DNA samples, set a negative control sterile double-distilled water and a positive control in each plate to control cross-contamination; seal the 384-well plate loaded with samples with a special membrane, and centrifuge for a certain period of time ; Insert the fluorescent quantitative PCR instrument, set the PCR reaction conditions; after the PCR reaction is completed, read the genotyping results.

[0104] Further, the specific steps are as follows:

[0105] 1. SNP typing

[0106] The substance obtained in Example 1 for detecti...

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Abstract

The invention discloses application of TNFSF11 (tumor necrosis factor superfamily member 11) gene rs9525641 SNP (single nucleotide polymorphism) or a genotype in human genomes in preparing products for detecting or screening hepatitis C chronicity or hepatitis C related SNP of the Chinese Han population. In practical use, substances for detecting polymorphism / allele / genetype of the rs9525641 can be combined with other substances (such as substances for detecting other SNP / allele / genotype substances related to hepatitis C) to prepare products for screening people with hepatitis C chronicity inthe Chinese Han population.

Description

[0001] The invention relates to the field of biomedicine, in particular to the preparation and detection of rs9525641 single nucleotide polymorphism (single nucleotide polymorphism, SNP) in human genome tumor necrosis factor superfamily member 11 (TNFRSF11) gene Application of Hepatitis C Chronic Products in Chinese Han Population. Background technique [0002] SNP is a single nucleotide variation that occurs at a specific position in the genome, including transition and transversion. Base conversion refers to the substitution between purines (adenine A, guanine G) or between pyrimidines (cytosine C, thymine T), and transversion refers to the substitution between purines and pyrimidines. SNPs may exist in both coding and non-coding regions of genes. There are two types of SNPs in the coding region: synonymous and nonsynonymous. SNPs with synonymous mutations do not affect the amino acid sequence of the protein. There are two types of non-synonymous SNPs: missense and nonsen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/70C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/707C12Q1/6858C12Q2600/156C12Q2600/172C12Q2531/113C12Q2561/101
Inventor 谈永飞岳明黄鹏李毓雯刘源李军凡豪志田亭符祖强巫晶晶张云
Owner YIXING PEOPLES HOSPITAL
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