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Application of asiatic acid to promotion of in vitro proliferation of human adipose mesenchymal stem cells and induction of chondrogenic differentiation of human adipose mesenchymal stem cells

A technology of asiatic acid and stromal stem cells, which is applied in asiatic acid to promote the proliferation of human adipose-derived mesenchymal stem cells in vitro and induce their chondrogenic differentiation.

Active Publication Date: 2019-12-20
刘兵
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] No asiatic acid and 2,3-dicarbonyl-23-carboxy n-butyl ester-ursane-12-ene-28-carboxylate ethyl ester, 2,3-dicarbonyl-23-carboxyisobutyl ester-urethane Effects of ethyl thane-12-ene-28-carboxylate on the proliferation and differentiation of ADSCs in vitro

Method used

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  • Application of asiatic acid to promotion of in vitro proliferation of human adipose mesenchymal stem cells and induction of chondrogenic differentiation of human adipose mesenchymal stem cells
  • Application of asiatic acid to promotion of in vitro proliferation of human adipose mesenchymal stem cells and induction of chondrogenic differentiation of human adipose mesenchymal stem cells
  • Application of asiatic acid to promotion of in vitro proliferation of human adipose mesenchymal stem cells and induction of chondrogenic differentiation of human adipose mesenchymal stem cells

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Embodiment Construction

[0023] The following describes the substantive content of the present invention in detail, but does not limit the protection scope of the present invention.

[0024] 1. Experimental materials

[0025] High-glucose DMEM medium was purchased from Gibco, USA.

[0026] Fetal bovine serum was purchased from Gibco, USA.

[0027] Fluorescence-labeled monoclonal antibodies were purchased from Bioscience, USA.

[0028] Asiatic acid, 2,3-dicarbonyl-23-carboxy n-butyl ester-ursane-12-ene-28-carboxylate ethyl ester (derivative A), 2,3-dicarbonyl-23-carboxyisobutyl Ester-ursane-12-ene-28-carboxylate ethyl ester (derivative B) is purchased or self-made, with a purity of not less than 98%.

[0029] Toluidine blue dye kit was purchased from Solarbio.

[0030] Adipogenic induction medium of human adipose-derived mesenchymal stem cells was purchased from Saiye Biotech.

[0031] Human adipose-derived mesenchymal stem cell osteogenesis induction medium was purchased from Saiye Biotech.

[0...

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Abstract

The invention discloses an application of asiatic acid to promotion of in vitro proliferation of human adipose mesenchymal stem cells and induction of chondrogenic differentiation of the human adiposemesenchymal stem cells. Researches discover that the effects of the asiatic acid, 2,3-dicarbonyl-23-carboxy n-butyl-ursane-12-ene-28-ethyl carboxylate and 2,3-dicarbonyl-23-carboxy isobutyl-ursane-12-ene-28-ethyl carboxylate on the human adipose mesenchymal stem cells have commonality and difference; the asiatic acid, the 2,3-dicarbonyl-23-carboxy n-butyl-ursane-12-ene-28-ethyl carboxylate and the 2,3-dicarbonyl-23-carboxy isobutyl-ursane-12-ene-28-ethyl carboxylate can significantly promote the in-vitro proliferation of the human adipose mesenchymal stem cells, but the asiatic acid can promote the chondrogenic differentiation of the human adipose mesenchymal stem cells while promoting the in-vitro proliferation of the human adipose mesenchymal stem cells, and is suitable for chondrogenesis of the human adipose mesenchymal stem cells, and the 2,3-dicarbonyl-23-carboxy n-butyl-ursane-12-ene-28-ethyl carboxylate and the 2,3-dicarbonyl-23-carboxy isobutyl-ursane-12-ene-28-ethyl carboxylate can maintain the stem cell characteristics of the human adipose mesenchymal stem cells while promoting the in vitro proliferation of the human adipose mesenchymal stem cells, and are suitable for rapid in vitro proliferation of the human adipose mesenchymal stem cells as seed cells.

Description

technical field [0001] The invention belongs to the field of biology, and relates to in vitro culture and expansion of adipose-derived mesenchymal stem cells, in particular to the use of asiatic acid for promoting the in vitro proliferation of human adipose-derived mesenchymal stem cells and inducing their chondrogenic differentiation. Background technique [0002] Adipose-derived mesenchymal stem cells (ADSCs) are a kind of stem cells with multi-directional differentiation potential isolated from adipose tissue, which can be induced to differentiate into fat, bone, cartilage, pancreatic β-cells and cardiac muscle under specific conditions. cell. [0003] In recent years, ADSCs have significantly increased each year in tissue repair and regeneration research. Researchers are most concerned about the number of ADSCs used in repair and regeneration, clinical trials evaluating efficacy and safety. According to the US Clinical Trials (2015) report, there are 121 studies regist...

Claims

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Application Information

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IPC IPC(8): C12N5/0775C12N5/077
CPCC12N5/0655C12N5/0667C12N2501/999
Inventor 杨姣姣
Owner 刘兵
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