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Purpose of asiatic acid derivatives for promoting in vitro proliferation of human ADSCs (Adipose-Derived Stem Cells) and maintaining stemness

A technology of asiatic acid and stem cells, applied in animal cells, vertebrate cells, microorganisms, etc.

Active Publication Date: 2019-12-20
湖南南华爱世普林生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] No asiatic acid and 2,3-dicarbonyl-23-carboxy n-butyl ester-ursane-12-ene-28-carboxylate ethyl ester, 2,3-dicarbonyl-23-carboxyisobutyl ester-urethane Effects of ethyl thane-12-ene-28-carboxylate on the proliferation and differentiation of ADSCs in vitro

Method used

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  • Purpose of asiatic acid derivatives for promoting in vitro proliferation of human ADSCs (Adipose-Derived Stem Cells) and maintaining stemness
  • Purpose of asiatic acid derivatives for promoting in vitro proliferation of human ADSCs (Adipose-Derived Stem Cells) and maintaining stemness
  • Purpose of asiatic acid derivatives for promoting in vitro proliferation of human ADSCs (Adipose-Derived Stem Cells) and maintaining stemness

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Embodiment Construction

[0023] The following describes the substantive content of the present invention in detail, but does not limit the protection scope of the present invention.

[0024] 1. Experimental materials

[0025] High-glucose DMEM medium was purchased from Gibco, USA.

[0026] Fetal bovine serum was purchased from Gibco, USA.

[0027] Fluorescence-labeled monoclonal antibodies were purchased from Bioscience, USA.

[0028] Asiatic acid, 2,3-dicarbonyl-23-carboxy n-butyl ester-ursane-12-ene-28-carboxylate ethyl ester (derivative A), 2,3-dicarbonyl-23-carboxyisobutyl Ester-ursane-12-ene-28-carboxylate ethyl ester (derivative B) is purchased or self-made, with a purity of not less than 98%.

[0029] Toluidine blue dye kit was purchased from Solarbio.

[0030] Adipogenic induction medium of human adipose-derived mesenchymal stem cells was purchased from Saiye Biotech.

[0031] Human adipose-derived mesenchymal stem cell osteogenesis induction medium was purchased from Saiye Biotech.

[0...

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Abstract

The invention discloses a purpose of asiatic acid derivatives for promoting in vitro proliferation of human ADSCs (Adipose-Derived Stem Cells) and maintaining stemness. Through studies, people discover that both similarities and differences exist on the effects of asiatic acid, 2,3-dicarbonyl-23-carboxyl n-butyl-ursane-12-ethenyl-28-oxocyclopentanecarboxylate and 2,3-dicarbonyl-23-carboxyl iso-butyl-ursane-12-ethenyl-28-oxocyclopentanecarboxylate on the human ADSCs; each of the asiatic acid, the 2,3-dicarbonyl-23-carboxyl n-butyl-ursane-12-ethenyl-28-oxocyclopentanecarboxylate and the 2,3-dicarbonyl-23-carboxyl iso-butyl-ursane-12-ethenyl-28-oxocyclopentanecarboxylate can obviously promote the in vitro proliferation of the human ADSCs, but the asiatic acid can also promote the chondrogenicdifferentiation of the human ADSCs while promoting the in vitro proliferation of the human ADSCs, and is suitable for an aspect of chondrogenic application of the human ADSCs; and the 2,3-dicarbonyl-23-carboxyl n-butyl-ursane-12-ethenyl-28-oxocyclopentanecarboxylate and the 2,3-dicarbonyl-23-carboxyl iso-butyl-ursane-12-ethenyl-28-oxocyclopentanecarboxylate can maintain the stem cell characteristics of the human ADSCs while promoting the in vitro proliferation of the human ADSCs, and is suitable for the in vitro fast proliferation of seed cells of the human ADSCs.

Description

technical field [0001] The invention belongs to the field of biology, and relates to in vitro culture and expansion of adipose-derived mesenchymal stem cells, in particular to an application of asiatic acid derivatives for promoting in vitro proliferation of human adipose-derived mesenchymal stem cells and maintaining stemness. Background technique [0002] Adipose-derived mesenchymal stem cells (ADSCs) are a kind of stem cells with multi-directional differentiation potential isolated from adipose tissue, which can be induced to differentiate into fat, bone, cartilage, pancreatic β-cells and cardiac muscle under specific conditions. cell. [0003] In recent years, ADSCs have significantly increased each year in tissue repair and regeneration research. Researchers are most concerned about the number of ADSCs used in repair and regeneration, clinical trials evaluating efficacy and safety. According to the US Clinical Trials (2015) report, there are 121 studies registered usi...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
CPCC12N5/0667C12N2500/30
Inventor 杨姣姣
Owner 湖南南华爱世普林生物技术有限公司
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