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A kind of injectable natural triterpenoid antibacterial hydrogel and preparation method thereof

A triterpene compound, injection-type technology, applied in the directions of antibacterial drugs, topical antibacterial agents, drug combinations, etc., can solve the problems of increased preparation cost and low application value, and achieves good bacteriostatic effect and high mechanical strength.

Active Publication Date: 2021-03-02
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method often requires cumbersome gel molecule synthesis and purification processes, which increases the cost of preparation and leads to low practical application value.

Method used

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  • A kind of injectable natural triterpenoid antibacterial hydrogel and preparation method thereof
  • A kind of injectable natural triterpenoid antibacterial hydrogel and preparation method thereof
  • A kind of injectable natural triterpenoid antibacterial hydrogel and preparation method thereof

Examples

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Embodiment 1

[0031] After in-depth research, the inventors prepared a triterpenoid hydrogel with inherent antibacterial properties and injectable properties. The present invention makes full use of the antibacterial properties and amphiphilic molecular structure of natural triterpenoids, and selects hydrogels with injectable and antibacterial properties through gelation experiments and research on antibacterial, cell activity and hemolysis experiments of hydrogels and preparation method.

[0032] In this example, a mixture of glycyrrhizic acid and glycyrrhetinic acid in pentacyclic triterpenoids is used as a gel molecule to prepare a hydrogel, and a PBS buffer with pH=7 is used as a solvent. Wherein, the mole fraction of glycyrrhizic acid is 70%, and the mole fraction of glycyrrhetic acid is 30%, and the specific preparation method is as follows:

[0033] First, a suspension solution of glycyrrhizic acid and glycyrrhizic acid with a concentration of 3.5 mM was prepared in a container, in ...

Embodiment 2

[0035] In this example, a mixture of glycyrrhizic acid and ursolic acid in pentacyclic triterpenoids is used as a gel molecule to prepare a hydrogel, and a PBS buffer with pH=7.4 is used as a solvent. Wherein, the mole fraction of glycyrrhizic acid is 60%, and the mole fraction of ursolic acid is 40%, and the specific preparation method is as follows:

[0036] First, prepare a suspension solution of glycyrrhizic acid and ursolic acid with a concentration of 5mM in a container, in which the mole fraction of glycyrrhizic acid is 60% and the mole fraction of ursolic acid is 40%; The solution was ultrasonically treated for 2 min to promote the uniform dispersion of glycyrrhizic acid and ursolic acid molecules in the aqueous solution; then the suspension solution was placed in an oven at 97 °C and heated for 150 min, at which time the solution changed from a turbid state to a transparent state; finally, the transparent solution was It was placed in an environment of 25° C. until th...

Embodiment 3

[0038] In this example, a mixture of glycyrrhizic acid and ginsenosides in tetracyclic triterpenoids is used as a gel molecule to prepare a hydrogel, and a PBS buffer with pH=7.2 is used as a solvent. Wherein, the mole fraction of glycyrrhizic acid is 90%, and the mole fraction of ginsenoside is 10%, and the specific preparation method is as follows:

[0039]First, a mixed suspension solution of 2mM glycyrrhizic acid and ginsenoside was prepared in a container with a pH=7.2 PBS buffer, wherein the mole fraction of glycyrrhizic acid was 90% and the mole fraction of ginsenoside was 10%; then at 37°C The suspension was ultrasonically treated for 15 min under the same environment to promote the two gel molecules to disperse evenly in the aqueous solution; then the suspension solution was placed in an oven at 90 °C and heated for 120 min, at which time the solution changed from a turbid state to a transparent state; finally The transparent solution was placed in an environment of 2...

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Abstract

The invention relates to the field of biological antibacterial hydrogel materials, in particular to an injectable natural triterpenoid antibacterial hydrogel and a preparation method thereof. The mixture of hydrogel glycyrrhizic acid and pentacyclic triterpenoid compound or tetracyclic triterpenoid compound disclosed in the present invention is a gel molecule, and phosphate buffered saline (PBS) is used as a gel solvent, and the non-co-coated structure formed between the mixtures is utilized. The valence bond force is prepared through the self-assembly mechanism of small molecules. The prepared hydrogel can exhibit antibacterial properties without adding antibacterial drugs, and the mechanical properties of the hydrogel are significantly improved. The preparation method of the hydrogel of the invention is simple, not only can simplify the preparation process of the injectable antibacterial hydrogel, but also expand the application field of the triterpenoids. It can be made into a new type of antibacterial material with biomedical application properties and mechanical strength.

Description

Technical field: [0001] The invention belongs to the field of biological antibacterial hydrogel materials, in particular to an injectable natural triterpenoid antibacterial hydrogel. Background technique: [0002] Low molecular weight hydrogels (referred to as hydrogels) are generally obtained by the formation of relatively stable three-dimensional network structures by small molecular compounds in water through non-covalent interactions. Among them, injectable hydrogels are widely used in tissue engineering, drug delivery, wound healing and other fields. However, because the application scenarios of hydrogels are mostly in humid environments, if they do not have antibacterial properties, they are prone to bacterial infection, which will eventually lead to serious infection of the target site. Therefore, the preparation of an injectable antibacterial hydrogel has become a research hotspot in the field of biomaterials. [0003] In view of the special properties and applicat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/06A61K31/704A61K31/56A61K31/575A61P31/04A61P31/02A61P17/02A61L26/00
CPCA61K9/0019A61K9/06A61K31/56A61K31/575A61K31/704A61L26/0061A61L26/0066A61L26/008A61L2300/404A61L2300/412A61L2400/06A61P17/02A61P31/02A61P31/04A61K2300/00
Inventor 胡君张浩吴剑桥于霞
Owner BEIJING UNIV OF CHEM TECH
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