Method for efficiently collecting and purifying Orobanchecumana germination stimuli by using mist culture and solid-phase extraction technologies

An extraction technology, solid-phase extraction cartridge technology, which is applied in the field of efficient collection and purification of sunflower rowan germination stimulants, can solve the problems of sunflower rowan germination stimulants such as few types, low concentration and purity, and achieve reduction in pretreatment process, High concentration and purity, the effect of improving purity and concentration

Active Publication Date: 2019-12-20
ZHEJIANG UNIV
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  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The object of the present invention is to solve the problem of less types, low concentration and low purity of the sunflower radang germination stimulant extracted and purified in the prior art by utilizing the method of efficiently collecting and purifying the sunflower radang germination stimulant by means of aeroponics and solid-phase extraction technology

Method used

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  • Method for efficiently collecting and purifying Orobanchecumana germination stimuli by using mist culture and solid-phase extraction technologies
  • Method for efficiently collecting and purifying Orobanchecumana germination stimuli by using mist culture and solid-phase extraction technologies
  • Method for efficiently collecting and purifying Orobanchecumana germination stimuli by using mist culture and solid-phase extraction technologies

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Embodiment 1

[0058] 1. Test method

[0059] (1) Seed preparation and treatment

[0060] Calculate the amount of seeds needed based on the germination rate of the seeds (test the germination rate in advance). In this experiment, 4 sunflower varieties (genotye) were selected, and each variety had 24 replicates. A total of 96 plants were needed, and about 100 sunflower seeds were prepared in advance for each variety.

[0061] (2) Nutrient solution configuration

[0062] The sunflower plant culture medium uses the improved 1 / 2 Hoagland's nutrient solution (half strength Hoagland's solution). The configuration of the modified 1 / 2 Hoagland's solution is shown in the table, and the final pH value is 5.8. The capacity and volume of the nutrient solution depends on the experimental situation. In this experiment, the concentrated solution of the nutrient solution is prepared first, bottled for later use, and then diluted for use. For example, use a plastic bucket with a volume capacity of 25L. ...

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Abstract

The invention discloses a method for efficiently collecting and purifying Orobanchecumana germination stimuli by using mist culture and solid-phase extraction technologies. The method includes the steps: taking sunflower seeds, performing germination, performing final planting on sunflower seedlings in a mist-culture device, and cultivating the sunflower seedlings by using the mist-culture system;and during mist culture, cultivating the sunflower seedlings firstly by using a phosphorus-containing mist-culture nutrient solution for 20-25 days, then performing starvation culture treatment by using a phosphorus-free mist-culture nutrient solution for 5-7 days, and performing solid-phase extraction on all the nutrient solutions in the mist-culture device through a solid-phase extraction column so as to obtain the Orobanchecumana germination stimuli. The sunflower seedlings are cultivated fast by using the mist-culture system firstly, then the Orobanchecumana germination stimuli in sunflower root exudates are separated and purified through combination of the mist-culture system with the solid-phase extraction technology, and the obtained Orobanchecumana germination stimuli have multiple varieties, high concentration and high purity.

Description

technical field [0001] The invention relates to the fields of plant cultivation technology and biochemical technology, in particular to a method for efficiently collecting and purifying sunflower rowidang germination stimulants by using aeroponics and solid-phase extraction technologies. Background technique [0002] Sunflower is one of the important oil crops in the world, and also one of the main oil crops in my country. Because of its high economic value, easy management, low cost, high benefit, and strong salt and alkali resistance, it is widely used in Northeast my country and It is widely planted in Northwest China, among which the Inner Mongolia Autonomous Region is the largest sunflower production area in my country, followed by Xinjiang. In recent years, with the increase of sunflower production, the parasitic weed Orobanchecumana has appeared in sunflower production areas, seriously endangering sunflower production, and the control of weeds is urgent. [0003] Para...

Claims

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Application Information

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IPC IPC(8): A01N43/12A01G31/00A01G22/00C05G1/00A01P21/00
CPCA01N43/12A01G31/00A01G22/00C05B7/00B01D15/325B01D15/1871B01D15/426B01D15/20A01G7/00B01D11/0492Y02P60/21A01G22/60A01G31/02A01N43/08A01N65/12B01D11/0288B01D11/0292B01D21/262
Inventor 胡露飏周伟军杨翀许玲章娜王建蘇朱金文白全江云晓鹏
Owner ZHEJIANG UNIV
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