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SSR primer set based on solanum melongena transcriptome sequencing data development, and application of SSR primer set

A technology of transcriptome sequencing and primer set, applied in the field of molecular biology, can solve the problems of low polymorphism of SSR markers and few SSR markers, and achieve the goal of promoting eggplant genetics and breeding, stable amplification and clear amplification bands. Effect

Active Publication Date: 2019-12-10
CROP RES INST OF FUJIAN ACAD OF AGRI SCI
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Problems solved by technology

At present, although a lot of research has been done on eggplant SSR at home and abroad, the number of existing eggplant SSR molecular markers is far from meeting the needs of eggplant molecular biology research. There are too few SSR markers available, and the polymorphism of SSR markers Therefore, developing a large number of SSR markers is still one of the important tasks in eggplant research

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  • SSR primer set based on solanum melongena transcriptome sequencing data development, and application of SSR primer set
  • SSR primer set based on solanum melongena transcriptome sequencing data development, and application of SSR primer set
  • SSR primer set based on solanum melongena transcriptome sequencing data development, and application of SSR primer set

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Embodiment Construction

[0044] The present invention will be further described below in conjunction with the examples, but not limited thereto.

[0045] 1. Transcriptome data sources

[0046] The eggplant transcriptome data comes from the results of the inventor's Illumina high-throughput deep sequencing of eggplant in 2019.

[0047] 2. Transcriptome SSR site identification and SSR primer design

[0048] Use the software MISA to search for SSR sites in the eggplant transcriptome. The search criteria are: the length of the repeat unit is 2-6 bp, and the minimum number of dinucleotides, trinucleotides, tetranucleotides, pentanucleotides and hexanucleotides The number of repetitions were 6, 5, 5, 4 and 4 times. The Primer 3.0 software was used to design and evaluate primers for the sequence before and after the SSR repeat unit, and each SSR generated 3 pairs of primers. The length of the primer sequence is 18-25bp. If the primer is too short, it cannot guarantee the uniqueness of the amplified sequen...

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Abstract

The invention discloses an SSR primer set based on solanum melongena transcriptome sequencing data development, and an application of the SSR primer set, and belongs to the technical field of molecular biology. The SSR primer set disclosed by the invention has 30 pairs of primers, wherein the nucleotide sequences of the primers from the first pair to the 30th pair are shown as SEQID No.1 to SEQIDNo.70, and the SSR primer set can be used for the fields of solanum melongena germplasm resource heredity diversity analysis, genetic relationship identification, DNA fingerprint spectrum constructionand the like. The obtained SSR primer set disclosed by the invention has the advantages of being rich in polymorphism, good in repeatability, stable in amplification, clear in electrophoresis stripsand the like; and besides, the primers are all from solanum melongena transcriptome expression genes, so that the variation of functional sequences in a genome can be reflected, and the diversity of solanum melongena heredity germplasms can be better distinguished.

Description

【Technical field】 [0001] The invention relates to the technical field of molecular biology, in particular to an SSR primer set developed based on eggplant transcriptome sequencing data and its application. 【Background technique】 [0002] Eggplant (Solanum elongena L.) is an important solanaceous vegetable crop in my country. It is planted all over the country and has important economic and nutritional value. China is the world's largest eggplant production and consumption country. In recent years, my country has made significant progress in the evaluation of eggplant germplasm resources, the innovation of breeding materials, the selection of new varieties, the genetic law, and the research on breeding techniques and methods. However, compared with other Solanaceae crops such as tomato and pepper, eggplant has a large gap in genetic diversity analysis, germplasm resource identification, molecular breeding, etc., especially the lack of a large number of polymorphic molecular ...

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 朱海生李永平刘建汀温庆放王彬陈敏氡叶新如张前荣
Owner CROP RES INST OF FUJIAN ACAD OF AGRI SCI
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