The ssr primer set developed based on eggplant transcriptome sequencing data and its application

A transcriptome sequencing and primer set technology, applied in the field of molecular biology, can solve the problems of low polymorphism of SSR markers and few SSR markers, and achieve the effects of clear amplification bands, stable amplification and rich polymorphisms.

Active Publication Date: 2022-03-18
CROP RES INST OF FUJIAN ACAD OF AGRI SCI
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Problems solved by technology

At present, although a lot of research has been done on eggplant SSR at home and abroad, the number of existing eggplant SSR molecular markers is far from meeting the needs of eggplant molecular biology research. There are too few SSR markers available, and the polymorphism of SSR markers Therefore, developing a large number of SSR markers is still one of the important tasks in eggplant research

Method used

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  • The ssr primer set developed based on eggplant transcriptome sequencing data and its application
  • The ssr primer set developed based on eggplant transcriptome sequencing data and its application
  • The ssr primer set developed based on eggplant transcriptome sequencing data and its application

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Embodiment Construction

[0044] The invention is further illustrated in connection with the examples, but is not limited thereto.

[0045] First, the source of transcription group data

[0046] The eggplant transcript data is derived from the inventors in 2019 to perform ILLUMINA high-throughput depth sequencing of eggplant in 2019.

[0047] Second, the transcription group SSR site identification and SSR primer design

[0048] Use software Misa to search for SSR sites in the eggplant transcription group, search criteria: repeating unit length 2 to 6 bp, dinucleotide, tri-nucleotide, tetrapliocide, pentuclearide and hexa nucleotide least The number of repetitions is 6, 5, 5, 4, and 4 times respectively. Primer 3.0 software The sequence before and after the SSR repeating unit was used to evaluate 3 pairs of primers per SSR. The primer sequence length is 18 to 25 bp, the primer is too short to ensure the uniqueness of the sequence, and the primer may be hybridized with the error pair of sequences, reducing s...

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Abstract

The invention discloses an SSR primer set developed based on eggplant transcriptome sequencing data and its application, which belongs to the technical field of molecular biology. The SSR primer set of the invention has 30 pairs of primers, and the nucleosides of the 1st to 30th pair of primers are The acid sequences are shown in SEQ ID No.1 to SEQ ID No.60; the SSR primer set can be used in the fields of genetic diversity analysis, phylogenetic relationship identification and DNA fingerprinting of eggplant quality resources. The SSR primer set obtained by the present invention has the advantages of rich polymorphism, good repeatability, stable amplification, and clear electrophoretic bands. At the same time, because these primers are all from the expression genes of the eggplant transcriptome, they can reflect the variation of the functional sequence in the genome, thereby It can better distinguish the diversity of eggplant genetic germplasm.

Description

[Technical field] [0001] The present invention relates to the field of molecular biology, and more particularly to an SSR primer group and its application based on eggplant transcription group sequencing data. 【Background technique】 [0002] Eggplant (Solanumm Elongena L.) is an important roast crop in my country. It has planted all over the country and has important economic and nutritional value. China is a country in the world's first eggplant production and consumption. In recent years, my country has made significant progress in the evaluation of eggplant germplasm resources, the innovation of breeding materials, the research of new varieties, genetic laws, breeding techniques and methods. However, compared with other eggplant such as tomato, pepper, etc., eggplant has a large gap in genetic diversity, germplating resources identification, molecular breeding, etc., especially lacking a large number of polymorphic molecular markers, resulting in eggplant molecules The study w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 朱海生李永平刘建汀温庆放王彬陈敏氡叶新如张前荣
Owner CROP RES INST OF FUJIAN ACAD OF AGRI SCI
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