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The ssr primer set developed based on the okra transcriptome sequence and its application

A technology of transcriptome sequence and primer set, which is applied in the biological field, can solve the problems such as the influence of the number of molecular markers, achieve clear amplification bands, promote the level of genetic breeding of okra, and have good repeatability

Active Publication Date: 2021-04-06
CROP RES INST OF FUJIAN ACAD OF AGRI SCI
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Problems solved by technology

At present, there are very few okra SSR reports, and the number of molecular markers affects the accuracy of genetic diversity analysis of germplasm resources and the accuracy of genetic map construction. The number of existing okra SSR molecular markers is far from meeting the requirements of okra molecular biology research. Therefore, the development of a large number of SSR markers is still one of the important tasks in okra research

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  • The ssr primer set developed based on the okra transcriptome sequence and its application
  • The ssr primer set developed based on the okra transcriptome sequence and its application
  • The ssr primer set developed based on the okra transcriptome sequence and its application

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Embodiment Construction

[0046] The present invention will be further described below in conjunction with the examples, but not limited thereto.

[0047] 1. Data source of okra transcriptome

[0048] Green okra resources were selected from the okra germplasm resource bank of the Crop Research Institute of Fujian Academy of Agricultural Sciences (the whole plant and tender fruit are green, with 6-8 ribs). The tender fruit 6 days after flowering was quick-frozen in liquid nitrogen at -80°C save. When sequencing, construct 1 library for each individual plant. Sequencing entrusted Guangzhou Jidio Biotechnology Co., Ltd. to use Illumina HiSeqTM 2500 PE125 system for RNA-Seq transcriptome sequencing (without reference), and Trinity was used for sequence assembly, and 3.87G of valid data was obtained after filtering.

[0049] 2. The distribution and structural characteristics of SSRs in the okra transcriptome

[0050] A total of 74,600 Unigenes (the total length of the sequence is about 52,976,011 bp) were ...

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Abstract

The invention provides an SSR primer set developed based on the okra transcriptome sequence and its application in identifying the kinship relationship and genetic characteristics of the okra, belonging to the field of biotechnology. The SSR primer set of the present invention has 36 pairs of primers, wherein the nucleotide sequences of the 1st to 36th pair of primers are shown in SEQ ID No.1-72. The SSR primer set obtained by the present invention has the advantages of rich polymorphism, good repeatability, stable amplification, and clear electrophoretic bands. At the same time, because these primers are all from the okra expression gene, they can reflect the variation of the functional sequence in the genome, thereby being more accurate. Good differentiation of genetic germplasm diversity in okra. The primer set can be used in the fields of genetic diversity analysis of okra quality resources, genetic relationship identification, DNA fingerprinting and other fields.

Description

technical field [0001] The invention relates to SSR primers, in particular to a set of SSR primers developed based on the okra transcriptome sequence and its application, belonging to the field of biotechnology. Background technique [0002] Okra ( Hibiscus esculentus L.), also known as croissant horn, is native to Northeast Africa and is cultivated in all countries in the world. China is mainly cultivated in Taiwan and Fujian. Okra fruit is rich in protein, multivitamins, minerals and rich dietary fiber, as well as flavonoids, polysaccharides and other ingredients. Okra has high health value: it can protect the retina and prevent cataracts; it can prevent cardiovascular diseases; it can promote gastrointestinal motility and prevent constipation; it can eliminate fatigue and restore physical strength quickly. Known as "gold", it is the first choice vegetable for athletes in many countries. Okra is rich in germplasm resources, widely distributed, and has many cultivars an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 李永平朱海生陈敏氡张前荣王彬刘建汀薛珠政温庆放
Owner CROP RES INST OF FUJIAN ACAD OF AGRI SCI
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