Chimeric antigen receptor and chimeric antigen receptor T cells targeting CD133, preparation method of chimeric antigen receptor T cells and application of chimeric antigen receptor and chimeric antigen receptor T cells

Abstract

The invention provides a chimeric antigen receptor CAR-CD133 targeting CD133 and chimeric antigen receptor T cells targeting the CD133. The CAR-CD133 comprises amino acid sequences of a single-chain antibody targeting CD133, an extracellular hinge region, a transmembrane region and an intracellular signal region which are sequentially connected from an amino terminal to a carboxyl terminal. The CAR-CD133 can specifically target CD133, takes a CD27 signal region as a co-stimulation signal region, activates T cells to play a role in cellular immunity, promotes amplification of the T cells in a patient body, efficiently and specifically kills CD133 positive tumor cells, and has lasting cell viability and lethality. The invention also provides a preparation method of the chimeric antigen receptor T cells targeting the CD133, a recombinant virus vector with the CAR-CD133 coding gene, and the like.

Description

technical field [0001] The invention relates to the field of medical biology, in particular to a CD133-targeted chimeric antigen receptor, chimeric antigen receptor T cells, and a preparation method and application thereof. Background technique [0002] Malignant tumor (cancer) has become the culprit threatening human life, and the incidence rate is on the rise. For example, the incidence of colorectal cancer ranks second among malignant tumors in Western countries. Although radiotherapy, chemotherapy, and surgery have significantly prolonged the lives of patients, there are still problems such as easy tumor recurrence and serious side effects. Evidence shows that the reason for tumor recurrence is that there are rare cells with significant proliferation ability in the tumor cell population, which are called tumor stem cells. This group of cells can differentiate into new tumors. Only killing them in tumor treatment can Eliminate tumor recurrence, and completely cure the tu...

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Problems solved by technology

For example, the incidence of colorectal cancer ranks second among malignant tumors in Western countries. Although radiotherapy, chemotherapy, and surgery have significantly prolonged the life of patients, there are still problems such as tumor recurrence and serious side effects.

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