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Hybrid breeding method of frozen sperms of little yellow croaker and large yellow croaker

A technology for small yellow croaker and large yellow croaker, applied in fish farming, preservation of human or animal body, climate change adaptation, etc., can solve the problem of high cost of human and material resources, and achieve the effect of overcoming reproductive isolation and realizing artificial hybridization.

Inactive Publication Date: 2019-11-26
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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Problems solved by technology

If two kinds of fish can be matured at the same period through temperature control (such as the Chinese invention patent with publication number CN 106172117A), hybrid offspring can be obtained through insemination after artificial induction, but the cost of manpower and material resources required for artificial temperature control is relatively high

Method used

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Examples

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Effect test

Embodiment 1

[0025] S1. Small yellow croaker sperm collection

[0026] In early April 2019, using 40 first-year-old male small yellow croakers bred in Xiangshan Gangwan Aquatic Seed Co., Ltd., when the small yellow croakers were sexually mature, the male small yellow croakers were injected with an oxytonic agent: LRH-A2 2.5µg / kg fish body weight, and after 12 hours of induced labor Select high-quality male small yellow croakers, anesthetize them with 1 mg / L alkylsulfonate ethyl aminobenzoate for 3 minutes, wrap the fish body with a moist clean towel to expose the reproductive pores, gently wipe the reproductive pores with absorbent paper, and gently press the abdomen. Collect sperm with a plastic straw, put them in 2mL cryovials, about 0.5mL per tube, add 2 times the volume of 4 ℃ pre-cooled sperm diluent containing antifreeze, fully invert and mix well, then let stand at room temperature for 3min, successfully obtained 38 Sperm of small yellow croaker (body weight 58.3g-64.5g), the sperm ...

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Abstract

The invention discloses a hybrid breeding method of frozen sperms of small yellow croaker and large yellow croaker, and belongs to the technical field of fry breeding of aquaculture marine fish. The method specifically comprises the following steps that small yellow croaker sperms are collected, a sperm dilute containing an anti-freezing agent is added in the small yellow croaker sperms, cryopreservation is conducted, large yellow croaker ovums are collected, the small yellow croaker sperms are thawed, and the sperms and the ovums are subjected to artificial insemination. According to the method, the sperms with the survival rate being 60% after thawing are selected to be crossed with the large yellow croaker ovums, the fertilization rate reaches 67.3%, and the hatching rate is 86.8% or above. The method has the important significance for overcoming reproductive isolation, achieving artificial hybridization, cultivating new varieties, and achieving large-scale fry breeding.

Description

technical field [0001] The invention belongs to the technical field of seed breeding of seawater fish in aquaculture, and in particular relates to a hybrid breeding method of frozen sperm of small yellow croaker and large yellow croaker. [0002] technical background [0003] Small yellow croaker and large yellow croaker belong to different species of fish, and there is a certain difference in the breeding water temperature between the two. The former is about 15°C as its suitable breeding water temperature, while the latter needs to be around 22°C to reproduce, which leads to the existence of the two. reproductive isolation. If two kinds of fish can be matured at the same time through temperature control (such as the Chinese invention patent with publication number CN 106172117A), hybrid offspring can be obtained by insemination after artificial induction, but the cost of manpower and material resources required for artificial temperature control is relatively high . Cont...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10A01K61/17A01N1/02
CPCA01N1/0221A01K61/10A01K61/17Y02A40/81
Inventor 刘峰陈红林楼宝詹炜谢庆平
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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