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Kit for detecting metastatic potential of colorectal cancer and use method thereof

A technology for colorectal cancer and metastatic potential, which is applied in the field of detection of colorectal cancer metastatic potential, can solve the problems of detection sensitivity and specificity, poor clinical effect of colorectal cancer, etc., achieve significant predictive value, improve medical level, The effect of improving the quality of life

Inactive Publication Date: 2019-11-22
成都和同易创生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] As far as colorectal cancer is concerned, in current clinical practice, the diagnosis of colon cancer usually includes observation of changes in bowel habits, blood in the stool, abdominal pain, etc., rectal index, laboratory tests include stool routine, fecal occult blood test, imaging examination, B-ultrasound, CT scan, colonoscopy, etc.; commonly used markers are carcinoembryonic antigen (CEA), colorectal cancer antigen (CCA), and CA19-9, but these antigen tests can generally only determine whether cancer is present or whether cancer has recurred. The clinical effect of judging or predicting the metastasis of colorectal cancer is poor, and the sensitivity and specificity of detection need to be improved

Method used

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  • Kit for detecting metastatic potential of colorectal cancer and use method thereof
  • Kit for detecting metastatic potential of colorectal cancer and use method thereof
  • Kit for detecting metastatic potential of colorectal cancer and use method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0036] The detection principle of the FGFR4 kit disclosed in the present invention is similar to the solid-phase sandwich enzyme-linked immunosorbent assay (ELISA), the enzyme-labeled secondary antibody is replaced by the fluorescently labeled antibody FITC-FGFR4, the standard substance with known concentration of the substance to be tested, the unknown Concentration samples were added to the microwell microtiter plate for detection. Firstly, the substance to be tested and FGFR4 monoclonal antibody were incubated simultaneously. After washing, add fluorescently labeled FITC-FGFR4 antibody, then incubate and wash to remove unbound FITC-FGFR4 antibody, then add fluorescence color enhancer, the fluorescence intensity is proportional to the concentration of the substance to be tested in the sample relation. The following are the specific experimental steps:

[0037] (1) Incline the blood sample test tube to the maximum slope, and pre-warm it for 20 minutes at room temperature or...

Embodiment 2

[0049] The correlation experiment between the expression of FGFR4 protein and the metastasis of colorectal cancer

[0050] 1. Collection of samples to be tested

[0051] The sample to be tested was prepared with reference to Example 1.

[0052] Analyze the expression of FGFR4 protein in the blood of 50 cases of primary colorectal cancer (non-metastasis) and 50 cases of colorectal cancer metastasis, in order to compare the difference in the expression of FGFR4 protein in the blood of primary and metastatic patients, and then determine the FGFR4 protein expression level. Correlation between expression levels and tumor metastasis.

[0053] 2. Determination of FGFR4 protein expression by ELISA-like method

[0054] (1) Incline the blood sample test tube to the maximum slope, and pre-warm it for 20 minutes at room temperature or in a 37°C incubator or water bath;

[0055] (2) Slowly peel off the blood clot from the tube wall and continue heating to promote the precipitation of se...

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Abstract

The invention relates to the field of the prediction of cancer metastasis and discloses a kit for detecting the metastatic potential of a colorectal cancer and a use method thereof. The kit includes apretreatment reagent for allowing a sample to be detected to fully expose an antigen, a detection reagent for detecting the expression quantity of FGFR4 protein in the sample to be detected, and FGFR4 protein standard products of different concentrations. The detection reagent includes a fluorescently-labeled FGFR4 antibody. The expression quantity of FGFR4 protein in blood is detected by immunofluorescence means, and the expression quantity is compared with an average value to judge the possibility of the metastasis of the colorectal cancer. The method has a significant predictive value forthe metastasis of a tumor of a colorectal cancer patient, so that early intervention can be carried out in a targeted way, the medical level can be improved, and the quality of life can be improved.

Description

technical field [0001] The invention relates to the field of prediction of cancer metastasis, and specifically discloses a kit for detecting the metastasis potential of colorectal cancer and a use method thereof. Background technique [0002] Metastatic cancer refers to the invasion of tumor cells from the primary site into lymphatic vessels, blood vessels or other pathways to it to continue to grow, forming a tumor of the same type as the primary site tumor, this process is called metastasis, and the formed tumor becomes a metastatic tumor or metastatic cancer. Metastasis is a hallmark of malignant tumors. The common ways of metastasis include lymphatic metastasis, vascular metastasis, implantation metastasis and so on. Once cancer cells invade the lymphatic vessels, they can fall off to form emboli, or proliferate in the tubes to form continuous tumors, but most of them enter the regional lymph nodes through the lymphatic vessels to form intra-lymph node metastasis. [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/68G01N33/58G01N33/535
CPCG01N33/57446G01N33/6893G01N33/582G01N33/535G01N2800/065
Inventor 黄灿华黄琛张涛
Owner 成都和同易创生物科技有限公司
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