Application of CNPY2 isomer 2 to diagnosis, prognosis, relapse and metastasis as well as radiotherapy and chemotherapy effect prediction of colorectal cancer

A technology for colorectal cancer and isomers, applied in the field of molecular biology detection, can solve problems such as missing the ideal period of treatment, and achieve the effect of improving medical level, strong specificity, and improving quality of life

Inactive Publication Date: 2018-09-21
SHENZHEN SENBOLL BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Before there are obvious clinical symptoms, such as blood in the stool, few people will take the initiative to request a colonoscopy, but it is often too late when symptoms of blood in the stool occur, and the ideal time for treatment is missed

Method used

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  • Application of CNPY2 isomer 2 to diagnosis, prognosis, relapse and metastasis as well as radiotherapy and chemotherapy effect prediction of colorectal cancer
  • Application of CNPY2 isomer 2 to diagnosis, prognosis, relapse and metastasis as well as radiotherapy and chemotherapy effect prediction of colorectal cancer
  • Application of CNPY2 isomer 2 to diagnosis, prognosis, relapse and metastasis as well as radiotherapy and chemotherapy effect prediction of colorectal cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Embodiment 1, the preparation of CNPY2 isomer 2 ELISA kit

[0064] 1. Development of Monoclonal Antibody (Mab)

[0065] Step 1. Animal Immunization

[0066] Six animals (3Balb / C mice+3C57 mice) were immunized with CNPY2 protein.

[0067] 1) Test phlebotomy: 7 days after each booster, the immune sera were used to test the immune response by ELISA. Bleeding was tested with target proteins and peptides by ELISA.

[0068] 2) Using antiserum, perform in-house testing.

[0069] 3) Maintain immunized animals until program completion.

[0070] Step 2. Cell Fusion and Screening

[0071] 1) Animal selection: According to the results of test bloodletting, the first two animals with the best immune response to the target peptide were selected for cell fusion. Fusion can be staggered.

[0072] 2) Cell fusion and clone plating: 2 fusions were performed by electrofusion. A fusion efficiency of approximately 1 hybridoma / 5000 B cells was observed. According to this experience, ...

Embodiment 2

[0171] Example 2, the results of the diagnostic efficacy of CNPY2 isomer 2

[0172] method:

[0173] (1) Select the crowd

[0174] 437 cases of colorectal cancer patients and 203 cases of non-malignant normal people, a total of 603 cases;

[0175] Among the 437 colorectal cancer patients, 108 were stage I, 110 were stage II, 108 were stage III, and 111 were stage IV.

[0176] (2) sample

[0177] 200 μl each of preoperative serum samples from colorectal cancer patients and normal population.

[0178] (3) Detection method

[0179] To establish a standard curve, add 100 μl of serum samples to the CNPY2 isomer 2 ELISA kit (from Shenzhen Shengboer Life Science Technology Co., Ltd.), according to the instructions, use a microplate reader to detect the OD value, and obtain the CNPY2 in the sample corresponding to the standard curve. Isoform 2 serum concentration.

[0180] (4) Statistical methods

[0181] SPSS 21.0 software was used for data analysis. The serum levels of CNPY2...

Embodiment 3

[0218] Embodiment 3, prognosis prediction result

[0219] method:

[0220] (1) Select the crowd

[0221] 425 patients with colorectal cancer (1. The primary tumor has been resected; 2. The follow-up time is > 3 months);

[0222] Among the 425 colorectal cancer patients, 107 were stage I, 104 were stage II, 105 were stage III, and 109 were stage IV.

[0223] (2) sample

[0224] 200 μl each of preoperative serum samples from colorectal cancer patients and normal population.

[0225] (3) Detection method

[0226] To establish a standard curve, add 100 μl of serum samples to the CNPY2 isomer 2 ELISA kit (from Shenzhen Shengboer Life Science Technology Co., Ltd.), according to the instructions, use a microplate reader to detect the OD value, and obtain the CNPY2 in the sample corresponding to the standard curve. Isoform 2 serum concentration.

[0227] (4) End point indicators

[0228] The whole group of patients: Overall survival (OS) was defined as the time interval from th...

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Abstract

The invention provides application of a CNPY2 isomer 2 to diagnosis, prognosis prediction as well as radiotherapy and chemotherapy effect prediction of colorectal cancer. An enzyme-linked immunosorbent assay (ELISA) is adopted to detect the concentration of the CNPY2 isomer 2 in human serum, and the detection result shows that the concentration of the CNPY2 isomer in the serum has diagnosis valuefor the colorectal cancer, has predictive value for prognosis of the total survival time of patients suffering from colorectal cancer III-IV, and also has predictive value for postoperative relapse and metastasis and pathological complete remission of the patients. Therefore, the invention provides an effective tool for diagnosis, prognosis, postoperative relapse and metastasis as well as radiotherapy and chemotherapy effect prediction of the colorectal cancer, and has great medical value and commercial value.

Description

technical field [0001] The invention belongs to the field of molecular biology detection. Specifically, the invention relates to the preparation of CNPY2 isoform 2 for diagnosing colorectal cancer, for predicting the overall survival time prognosis of colorectal cancer patients, and for predicting the operation of colorectal cancer patients after radiotherapy and chemotherapy. Use in a kit for predicting whether the pathology of colorectal cancer patients can be completely remitted after recurrence and metastasis. The present invention also relates to the above-mentioned kits. Background technique [0002] Colorectal cancer (carcinoma of colon and rectum) is a common malignant tumor in the gastrointestinal tract. The early symptoms are not obvious. With the increase of the cancer, symptoms such as changes in bowel habits, blood in the stool, diarrhea, alternating diarrhea and constipation, and local abdominal pain In the late stage, systemic symptoms such as anemia and weig...

Claims

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Application Information

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IPC IPC(8): G01N33/574
CPCG01N33/57419G01N33/57488G01N2333/47
Inventor 郭健万德森方淯靖彭健宏区庆坚潘志忠
Owner SHENZHEN SENBOLL BIOTECH CO LTD
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