Method for detecting fat-soluble vitamins in blood sample
A fat-soluble vitamin and blood sample technology, applied in the field of vitamin detection, can solve the problems of long detection time, long analysis time, sample loss, etc., achieve strong anti-matrix interference ability, simple and fast operation, and reduce analysis errors.
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[0037] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.
[0038] (1) Prepare the sample to be tested
[0039] Add a methanol solution containing a variety of fat-soluble vitamin internal standards to the serum sample for protein precipitation, vortex for 3 minutes, add n-hexane extraction solvent, vortex for 5 minutes, centrifuge at 11000rpm at 4°C for 15 minutes, and then transfer After the supernatant was taken and dried, the reconstitution solvent was added, vortexed for 2 minutes, and centrifuged at 11,000 rpm for 3 m...
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