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Glow-type chemiluminescence sensor constructed based on hydrogel and preparation method and application thereof

A chemiluminescence and hydrogel technology, which is applied in the direction of chemiluminescence/bioluminescence, and analysis by making materials react chemically, can solve the problems of limited application, complex electrode modification process equipment and other problems, and achieve enhanced chemiluminescence intensity , long chemiluminescent time and short detection time

Active Publication Date: 2019-11-22
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of these methods have complicated electrode modification process and complex instruments and equipment, which limit the application of these methods

Method used

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  • Glow-type chemiluminescence sensor constructed based on hydrogel and preparation method and application thereof
  • Glow-type chemiluminescence sensor constructed based on hydrogel and preparation method and application thereof
  • Glow-type chemiluminescence sensor constructed based on hydrogel and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 ABEI / Co 2+ Synthesis of / CS hydrogel

[0050] In 15mL alkaline solution (mass ratio is LiOH: KOH: urea: H 2 (O=4.5:7:8:80.5) stirring and dispersing 0.4g chitosan powder, adding 0.6mL CoCl 2 (1mM) solution, stirred for 5 minutes, and refrigerated at -20°C until completely frozen. After thawing at room temperature, 1.5 mL of ABEI (10 mM) solution was added and stirred for 5 hours to uniformly disperse in the system to obtain ABEI / Co 2+ / CS hydrogel and store at 4 °C.

[0051] figure 2 A shows the synthetic ABEI / Co 2+ / CS SEM image of the hydrogel; the characteristic absorption peak of ABEI is located at the red line at 291nm, and the ultraviolet-visible absorption spectrum proves that ABEI is successfully wrapped in the hydrogel.

Embodiment 2

[0052] Example 2 ABEI / Co 2+ Synthesis of / CS hydrogel

[0053] In 15mL alkaline solution (mass ratio is LiOH: KOH: urea: H 2 (O=4.5:7:8:80.5) stirring and dispersing 0.4g chitosan powder, adding 0.6mL CoCl 2 (0.1 mM) solution, stirred for 5 minutes, and refrigerated at -20°C until completely frozen. After thawing at room temperature, add 1.5mL ABEI (4mM) solution and stir for 5 hours to be uniformly dispersed in the system to obtain ABEI / Co 2+ / CS hydrogel and store at 4 °C.

Embodiment 3

[0054] Example 3 Synthesis of MOF-Pt

[0055] First, weigh 0.2g of TCPP, 0.12g of AlCl 3 ·3H 2 The CTAB of O and 0.144g was dissolved in 20mL water, stirred for 10 minutes and placed in a 20mL reactor, then reacted at 180°C for 16 hours, after centrifuging at 3000rpm, the supernatant was centrifuged at 13000rpm to obtain a precipitate. The precipitate was washed twice with DMF, water and acetone, respectively, and then dried overnight in vacuum. Then, purple and black precipitates were obtained by vacuum drying at 150°C for 7 hours for further reaction. Then weigh 0.038g of precipitate and 0.064g of Cu(Ac) 2 It was dissolved in 8ml of DMF organic solvent, stirred for 10 minutes, then poured into a 20mL reaction kettle, and reacted at 100°C for 24 hours to obtain a red-black blocky precipitate. After centrifugation at 13000rpm, the precipitate was washed twice with DMF, water and acetone, and then dried in vacuo overnight to obtain product 1.

[0056] Finally, 0.5ml of H ...

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Abstract

The invention discloses a glow-type chemiluminescence sensor constructed based on hydrogel and a preparation method and application thereof. The invention also discloses an analytical method for detecting organophosphorus pesticides by using the glow-type chemiluminescence sensor constructed based on hydrogel, which comprises the following steps: (1) preparing ABEI / CO<2+> / CS hydrogel; (2) preparing an MOF-Pt material; and (3) mixing an organophosphorus pesticide with acetylcholinesterase for reaction to obtain mixed solution, adding chlorinated acetylcholine solution, choline oxidase solutionand PBS buffer solution into the mixed solution for reaction to obtain a mixture, adding the MOF-Pt and the mixture into the hydrogel, and measuring the chemiluminescence intensity with a chemiluminescence imager. The analytical method does not need the aid of precise and expensive experimental instruments, has no strict and complex experimental operation process, greatly reduces the detection cost of organophosphorus pesticides, and has the advantages of high sensitivity, short detection time, long luminescent time, high-throughput detection and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a glow-type chemiluminescent sensor constructed on the basis of hydrogel and its preparation method and application. Background technique [0002] Pesticides are the main substances used in the modern agricultural industry to prevent, regulate or kill other organisms that harm crop growth. Organophosphorus pesticides (OPs) have been widely used in agriculture and forestry because of their high insecticidal efficiency, high crop yield, and easy degradation. However, most organophosphate pesticides are highly toxic. Excessive or improper use will cause environmental pollution. Because OPS can inhibit the activity of acetylcholinesterase (AChE), the central nervous system is seriously disturbed, causing harm to human health, and even causing death in severe cases. Therefore, the rapid and sensitive detection of OPS is of great significance to life health and environmental protection....

Claims

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Application Information

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IPC IPC(8): G01N21/76C08J3/075C08L5/08C08K3/16C08K5/24
CPCC08J3/075C08J2305/08C08K3/16C08K5/24G01N21/76
Inventor 卫伟陆叶卫敏刘松琴
Owner SOUTHEAST UNIV
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