UPLC characteristic chromatogram construction method and identification method of rhizoma cibotii medicinal material and scalded rhizoma cibotii medicinal material
A technology of feature map and construction method, which is applied to the construction method and identification field of UPLC feature map of Goujie and scalded gouji medicinal materials, can solve the problems of deviation of conclusions, false positives, limitations, etc., and achieve the effect of stable specificity and stable baseline.
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Embodiment 1
[0036] Construction method of UPLC characteristic map of Gouji and Tanggouji medicinal materials
[0037] experiment method
[0038] Preparation of reference solution
[0039]Take an appropriate amount of protocatechuic acid reference substance and 5-hydroxymethylfurfural reference substance, weigh them accurately, add methanol to make a mixed solution containing 20 μg of protocatechuic acid and 50 μg of 5-hydroxymethylfurfural per 1 ml, shake well, that is have to.
[0040] Preparation of dog ridge and hot dog ridge test solution
[0041] Take 1g of dog ridge and scalded dog ridge powder, weigh it accurately, put it in a stoppered conical flask, add 25ml of mixed solution precisely, weigh it, treat it ultrasonically for 30 minutes, let it cool, weigh it again, and use the mixed solution to supplement the reduced amount. Lost weight, shake well, filter, take the continued filtrate, that is.
[0042] The mixed solution is composed of methanol and 1% glacial acetic acid solu...
Embodiment 2
[0065] Randomly buy a batch of dog spine medicinal materials from the market to identify its authenticity.
[0066] A method for identifying dog ridge and scalded dog ridge medicinal materials, the measurement steps are as follows:
[0067] (1) Accurately weigh the medicinal material of dog spine to be identified, and prepare the sample solution of dog spine to be identified;
[0068] (2) Precisely absorb the sample solution of dog ridge to be identified, inject it into an ultra-high performance liquid chromatograph, measure it, and get it;
[0069] (3) Compare the measured UPLC characteristic map with the constructed dog-ridge UPLC characteristic map. If it is consistent with the dog-ridge characteristic map, the sample to be tested is dog-ridge.
[0070] Preparation of reference solution
[0071] Take an appropriate amount of protocatechuic acid reference substance and 5-hydroxymethylfurfural reference substance, weigh them accurately, add methanol to make a mixed solution...
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