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UPLC characteristic chromatogram construction method and identification method of rhizoma cibotii medicinal material and scalded rhizoma cibotii medicinal material

A technology of feature map and construction method, which is applied to the construction method and identification field of UPLC feature map of Goujie and scalded gouji medicinal materials, can solve the problems of deviation of conclusions, false positives, limitations, etc., and achieve the effect of stable specificity and stable baseline.

Active Publication Date: 2019-11-12
GUANGDONG YIFANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At this stage, the traditional simple methods such as seeing, tasting, sniffing, and touching have been very limited in the identification of Chinese herbal medicines, and there are a large number of false positives and false negatives in chromatographic methods such as HPLC, IR, NIR, and TLC, which can easily lead to bias in conclusion

Method used

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  • UPLC characteristic chromatogram construction method and identification method of rhizoma cibotii medicinal material and scalded rhizoma cibotii medicinal material
  • UPLC characteristic chromatogram construction method and identification method of rhizoma cibotii medicinal material and scalded rhizoma cibotii medicinal material
  • UPLC characteristic chromatogram construction method and identification method of rhizoma cibotii medicinal material and scalded rhizoma cibotii medicinal material

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Construction method of UPLC characteristic map of Gouji and Tanggouji medicinal materials

[0037] experiment method

[0038] Preparation of reference solution

[0039]Take an appropriate amount of protocatechuic acid reference substance and 5-hydroxymethylfurfural reference substance, weigh them accurately, add methanol to make a mixed solution containing 20 μg of protocatechuic acid and 50 μg of 5-hydroxymethylfurfural per 1 ml, shake well, that is have to.

[0040] Preparation of dog ridge and hot dog ridge test solution

[0041] Take 1g of dog ridge and scalded dog ridge powder, weigh it accurately, put it in a stoppered conical flask, add 25ml of mixed solution precisely, weigh it, treat it ultrasonically for 30 minutes, let it cool, weigh it again, and use the mixed solution to supplement the reduced amount. Lost weight, shake well, filter, take the continued filtrate, that is.

[0042] The mixed solution is composed of methanol and 1% glacial acetic acid solu...

Embodiment 2

[0065] Randomly buy a batch of dog spine medicinal materials from the market to identify its authenticity.

[0066] A method for identifying dog ridge and scalded dog ridge medicinal materials, the measurement steps are as follows:

[0067] (1) Accurately weigh the medicinal material of dog spine to be identified, and prepare the sample solution of dog spine to be identified;

[0068] (2) Precisely absorb the sample solution of dog ridge to be identified, inject it into an ultra-high performance liquid chromatograph, measure it, and get it;

[0069] (3) Compare the measured UPLC characteristic map with the constructed dog-ridge UPLC characteristic map. If it is consistent with the dog-ridge characteristic map, the sample to be tested is dog-ridge.

[0070] Preparation of reference solution

[0071] Take an appropriate amount of protocatechuic acid reference substance and 5-hydroxymethylfurfural reference substance, weigh them accurately, add methanol to make a mixed solution...

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Abstract

The invention relates to a UPLC characteristic chromatogram construction method and an identification method of a rhizoma cibotii medicinal material and a scalded rhizoma cibotii medicinal material. The UPLC characteristic chromatogram construction method of the rhizoma cibotii medicinal material and the scalded rhizoma cibotii medicinal material comprises the steps that (1) the rhizoma cibotii medicinal material and the scalded rhizoma cibotii medicinal material are precisely weighed, and a rhizoma cibotii sample solution and a scalded rhizoma cibotii sample solution are prepared; and (2) therhizoma cibotii sample solution and the scalded rhizoma cibotii sample solution are precisely sucked and injected into an ultrahigh-performance liquid chromatograph for analysis, and a UPLC characteristic chromatogram of the rhizoma cibotii medicinal material and a UPLC characteristic chromatogram of the scalded rhizoma cibotii medicinal material are obtained. The construction method is accurate,reliable, easy and convenient to operate and good in reproducibility. The rapid, accurate and objective method is provided for identification of the rhizoma cibotii medicinal material and the scaldedrhizoma cibotii medicinal material, the rhizoma cibotii medicinal material and the scalded rhizoma cibotii medicinal material can be distinguished fundamentally and quickly, and the method has a certain characteristic.

Description

technical field [0001] The invention belongs to the field of detection of traditional Chinese medicines, and in particular relates to a construction method and identification method of UPLC characteristic maps of dog ridge and hot dog ridge medicinal materials. Background technique [0002] Dog ji is derived from the dry rhizome of Cibotium barometz ( L. ) J. Sm., a large fern in the clam shell family Fernaceae. It is also known as golden dog, golden dog cane, golden hericium, and Fujin. Bitter and cold, it has the effects of clearing away heat and detoxification, cooling blood and eliminating spots, etc. It is mostly used after processing, and has the effects of dispelling rheumatism, nourishing the liver and kidney, and strengthening the waist and knees. It is mainly used for rheumatic arthralgia, soreness of the waist and knees, and weakness of lower limbs. Medication history of dog ridge The name of dog ridge was first recorded in "Shen Nong's Materia Medica". unanimo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 杨敏娟雷桥仕谭吉娇王燕雄何嘉莹陈向东孙冬梅魏梅程学仁
Owner GUANGDONG YIFANG PHARMA
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