A system and its application method for increasing the transplanting emergence of test-tube seedlings of grapes

Abstract

The invention relates to the technical field of plant tissue culture application, in particular to a system and an application method for increasing the transplanted seedling emergence of "King Violet" grape test-tube seedlings. Cut the young shoots of the grape plants, remove the leaves, cut them into single-bud stems, inoculate them into the culture medium after sterilization, and obtain test-tube seedlings suitable for in vitro culture after subculture. Transplanting the lower section and cutting the upper section, the test-tube seedlings changed from one to two, and the "Violet King" grape test-tube seedling transplanting system controls the temperature, humidity, and light in the small plastic shed, which not only increases the number of grape test-tube seedlings. The emergence of domesticated transplanted seedlings improves the survival rate of test-tube seedlings of grapes, reduces production costs, can also reduce the height of tissue cultured seedlings, and overcomes the disadvantage of inconvenient management of creeping growth after transplanting.

Description

A system and application method for increasing the transplanted seedlings of grape test-tube seedlings technical field The present invention relates to plant tissue culture application technology field, be specifically related to a kind of increase " violet king " grape test A system for tube seedling transplanting emergence quantity and an application method thereof. Background technique China is the world's largest grape producing country, but at present there are problems such as single main planting variety, concentrated ripening period, with the market With the development of the market economy and the improvement of people's requirements for grape quality, the demand for new varieties is increasingly urgent. "King Violet" (also "Red King") is a new grape variety cultivated in Japan, which is a hybrid of "Weike × Sunshine Rose (or Wenke × Sunshine Rose)" Breeding, single fruit weight 25-30g, dark red skin color, easy to color, sugar content up to 23%, strong fr...

AI Technical Summary

Problems solved by technology

However, plant tissue culture is highly specialized, requires high standards for production sites, complicated instruments, equipment, and reagent supplies, and requires experienced technicians to cultivate and manage them. These are the factors that cause the high cost of tissue culture.

In addition, the general test-tube seedling height of 1.5-3cm can meet the transplanting requirements, but when the new grape variety "Violet King" is rapidly propagated and transplanted in bottles using tissue culture technology, the test-tube seedling height can reach 6-8cm, and Grapes are vine fruit trees that cannot grow upright

During the domestication and transplanting in the greenhouse, in order to improve the utilization rate of the facilities, the transplanting containers out of the bottles are small and densely placed, and the grape seedlings grow prostrate, which makes the management at the seedling stage inconvenient

The technical problem to be solved in the present invention is to increase the seedling emergence rate of domesticated and transplanted grape test-tube seedlings, improve the survival rate of grape test-tube seedlings, reduce costs, reduce the height of transplanted seedlings at the same time, and reduce the creeping growth of grapes transplanted into living seedlings to a certain extent. Problems that cause inconvenient management

Images