Extraction method of succinic acid

An extraction method, the technology of succinic acid, applied in the chemical industry, can solve the problems of complex reaction conditions, electrode corrosion, high power consumption of electrolytic synthesis, etc., and achieve the effect of high extraction yield and high purity

Inactive Publication Date: 2019-11-01
广州怡和生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the production methods of succinic acid with industrial application prospects mainly include electrolytic synthesis, catalytic hydrogenation and biological fermentation. Among them, electrolytic synthesis has problems such as high power consumption and electrode corrosion; while catalytic hydrogenation is the current industrial The most widely used synthesis method of succinic acid has the advantages of high yield and good selectivity, but its reaction conditions are complicated and the by-products pollute the environment; Chemical synthesis methods with harsh conditions and by-products that easily pollute the environment will eventually be eliminated by the times; while biological fermentation methods with mild conditions, environmental friendliness, and low pollution will eventually become the mainstream in the future

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  • Extraction method of succinic acid

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Embodiment 1

[0033] The present embodiment provides a kind of extraction method of succinic acid, comprises the steps:

[0034] (1) Insert Actinobacillus succinicum into the liquid seed culture medium, and culture it statically at 37°C for 14 hours, wherein, the composition of the seed culture medium, in terms of mass concentration, includes: glucose 35g / L, corn slurry 20g / L, peptone 7g / L, potassium dihydrogen phosphate 8g / L, sodium dihydrogen phosphate 12g / L, yeast extract 12g / L, deionized water;

[0035] (2) Move the actinobacillus succinic acid cultured through the seed culture medium into the fermentation medium, and obtain the succinic acid fermentation liquid through anaerobic cultivation, wherein, the composition of the fermentation medium, in terms of mass concentration, includes: Glucose 30g / L, corn steep liquor 25g / L, yeast extract 10g / L, sodium bicarbonate 3g / L, magnesium chloride 1g / L, calcium chloride 0.5g / L, basic magnesium carbonate 30g / L, deionized water, The inoculum size...

Embodiment 2

[0040] The present embodiment provides a kind of extraction method of succinic acid, comprises the steps:

[0041] (1) Insert Actinobacillus succinicum into the liquid seed culture medium and culture it statically at 37°C for 16 hours, wherein the components of the seed culture medium include: glucose 30g / L, corn slurry 20g / L, peptone 7g / L, potassium dihydrogen phosphate 6g / L, sodium dihydrogen phosphate 10g / L, yeast extract 10g / L, deionized water;

[0042] (2) Move the actinobacillus succinic acid cultured through the seed culture medium into the fermentation medium, and obtain the succinic acid fermentation liquid through anaerobic cultivation, wherein, the composition of the fermentation medium, in terms of mass concentration, includes: Glucose 35g / L, corn steep liquor 20g / L, yeast extract 12g / L, sodium bicarbonate 3.5g / L, magnesium chloride 1g / L, calcium chloride 0.6g / L, basic magnesium carbonate 25g / L, deionized water , the inoculum size of Actinobacillus succinate is 10...

Embodiment 3

[0047] The present embodiment provides a kind of extraction method of succinic acid, comprises the steps:

[0048] (1) Insert Actinobacillus succinicum into the liquid seed culture medium and culture it statically at 37°C for 12 hours, wherein the composition of the seed culture medium includes: glucose 32g / L, corn slurry 25g / L, peptone 6g / L, potassium dihydrogen phosphate 8g / L, sodium dihydrogen phosphate 10g / L, yeast extract 10g / L, deionized water;

[0049] (2) Move the actinobacillus succinic acid cultured through the seed culture medium into the fermentation medium, and obtain the succinic acid fermentation liquid through anaerobic cultivation, wherein, the composition of the fermentation medium, in terms of mass concentration, includes: Glucose 30g / L, corn steep liquor 15g / L, yeast extract 10g / L, sodium bicarbonate 2g / L, magnesium chloride 1g / L, calcium chloride 0.3g / L, basic magnesium carbonate 25g / L, deionized water, The inoculum size of Actinobacillus succinate was 5%...

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Abstract

The invention discloses an extraction method of succinic acid. The method comprises the following steps: performing anaerobic fermentation by adopting actinobacillus succinogenes to obtain succinic acid fermentation liquor; carrying out pretreatment to remove solid particles and macromolecular impurities in the succinic acid fermentation liquor, carrying out adsorption and elution treatment through a one-stage adsorption column to obtain succinic acid eluent and remove pigments, proteins, saccharides, inorganic salt ions and micromolecular acids generated by anaerobic fermentation; and finally, carrying out vacuum concentration, cooling crystallization, filtration, drying and the like to obtain succinic acid. The extracted succinic acid product is high in purity and high in extraction yield.

Description

technical field [0001] The invention relates to the technical field of chemical industry, in particular to a method for extracting succinic acid. Background technique [0002] Succinic acid commonly known as succinic acid, molecular formula C 4 h 6 o 4 , relative molecular mass 118.09, structural formula HOOC-CH 2 -CH 2 -COOH, succinic acid is a common natural organic acid, which widely exists in a variety of plants and human and animal tissues, for example, immature grapes, sugar beets and human blood and muscle. Succinic acid is an important organic chemical raw material and intermediate, mainly used in the production of erythromycin ethylsuccinate in the pharmaceutical industry, plant growth regulators, fungicides in agricultural production, and advanced organic pigment phthalocyanine red in the dye industry; In the food industry, succinic acid can be used as a flavor improver for soy sauce, liquid condiments and refined products. [0003] At present, the production...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C51/42C07C55/10C12P7/46
CPCC07C51/42C12P7/46C07C55/10
Inventor 李海王夏燕沈泽璇郑文辉
Owner 广州怡和生物科技有限公司
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