Chaetomium thermophilum temperature-resistant catalase and application

A technology of catalase and thermophilic Chaetomium, applied in the field of genetic engineering, can solve the problems of poor high temperature resistance of CAT, achieve good high temperature and high humidity resistance, accelerate popularization and application, and reduce market price Effect

Inactive Publication Date: 2019-10-25
SHANGHAI YOUTELL BIOCHEM +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The extraction of CAT by fermentation has begun to attract attention, and many companies have begun to research and develop related high-efficiency expression strains and fermentation extraction processes, but the existing CAT has poor high temperature resistance and cannot meet the needs

Method used

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  • Chaetomium thermophilum temperature-resistant catalase and application
  • Chaetomium thermophilum temperature-resistant catalase and application
  • Chaetomium thermophilum temperature-resistant catalase and application

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Embodiment

[0027] 1. Discovery of the CatA gene of Chaetomium thermophiles

[0028] By using the amino acid sequence of Aspergillus niger (Aspergillus niger) catalase Cat-R (Fowler et al., 1993) as a template, a search for Chaetomiumthermophilum DSM1495 in the US Center for Biotechnology Information (NCBI) showed that there are three Genes with unknown functions (accession numbers CTHT_0024820, CTHT_0021320 and CTHT_0030860) have relatively low homology (33-57%), and the amino acid residue numbers of these three proteins are 723, 776 and 583, respectively.

[0029] 2. Expression and secretion of Chaetomium thermophilia CatA gene in Trichoderma

[0030]The protein and corresponding gene of Chaetomium thermophila are respectively named as CatA and catA, and the amino acid sequence is shown in SEQID No.1, which contains 2172 codon reverse transcriptions according to the highly expressed gene of Trichoderma reesei The deoxyribonucleic acid (DNA) sequence of the base pair, in order to facili...

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Abstract

The invention discloses chaetomium thermophilum temperature-resistant catalase and application. An amino acid sequence of chaetomium thermophilum temperature-resistant catalase is shown as SEQ ID No.1. Chaetomium thermophilum temperature-resistant catalase is obtained through heterogeneous expression and fermenting production of chaetomium thermophilum temperature-resistant catalase CatA gene, hashigh resistance to high temperature and high humidity and can be used for replacing antibiotics in livestock-poultry breeding.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and in particular relates to a heat-resistant catalase and its application. Background technique [0002] Catalase (hydrogen peroxidase) is a kind of terminal oxidase widely present in animals, plants and microorganisms, and it is one of the key enzymes in the biological defense system established in the process of biological evolution. Catalase was first discovered by Thenard in 1811. In 1900, Loew named this enzyme "catalase", that is, catalase, also known as catalase (CAT), and found that this enzyme exists in many animals and plants, and its main function is to clear excess peroxidation hydrogen. A large number of experiments subsequently proved that CAT exists in various organisms, and has a clear H in the organism. 2 o 2 important functions. Early studies on catalase mainly focused on the chemical properties of the enzyme. With the rapid development of molecular biology and genetic e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/08C12N15/53C12N15/80C12N1/15A23K20/189A23K50/75C12R1/885C12R1/645
CPCA23K20/189A23K50/75C12N9/0065C12N15/80C12Y111/01006
Inventor 李新良张民刘睿张扬
Owner SHANGHAI YOUTELL BIOCHEM
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