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Determination method for ammonium nitrogen, nitric nitrogen, nitrite nitrogen and total dissolved nitrogen in samples

A method of determination, technology of ammonium nitrogen, applied in color/spectral characteristic measurement, material analysis by observing the influence of chemical indicators, analysis by making materials undergo chemical reactions, etc., can solve the detection limit and accuracy The speed and uniformity of the limited reaction need to be improved to achieve the effect of high accuracy, low detection limit and low detection limit

Inactive Publication Date: 2019-10-18
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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Problems solved by technology

[0002] The method for measuring the nitrogen content in soil in the prior art mainly relies on the ordinary UV-Vis spectrophotometer, which can only measure a limited number of samples at a time
Moreover, limited by the speed and uniformity of the reaction during the assay, it cannot be realized on a 96-well plate (microplate reader).
In addition, relying solely on ordinary UV-visible spectrophotometers for measurement, the detection limit and accuracy need to be improved

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  • Determination method for ammonium nitrogen, nitric nitrogen, nitrite nitrogen and total dissolved nitrogen in samples
  • Determination method for ammonium nitrogen, nitric nitrogen, nitrite nitrogen and total dissolved nitrogen in samples
  • Determination method for ammonium nitrogen, nitric nitrogen, nitrite nitrogen and total dissolved nitrogen in samples

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preparation example Construction

[0037] In the present invention, the mass ratio of sodium hypochlorite to sodium chloroisocyanurate in the oxidizing agent is preferably 6.5-7.2:0.025-0.035. In a specific embodiment of the present invention, the preparation method of the oxidizing agent preferably includes the following steps: measure 65mL of sodium hypochlorite solution (available chlorine content > 5.3%), and dissolve it in 1000mL of water; Sodium isocyanurate, standing overnight, yields the oxidizing agent.

[0038] In a specific embodiment of the present invention, the preparation method of the color developer preferably includes the following steps: weighing 144 g of sodium salicylate and dissolving it in 1000 mL of NaOH solution with a concentration of 0.3 mol / L to obtain the color developer.

[0039] In the present invention, the order of mixing the ammonium nitrogen standard solution, the oxidizing agent and the color developing agent is preferably that the ammonium nitrogen standard solution is mixed...

Embodiment 1

[0087] Preparation of soil solution: Take fresh soil and pass it through a 2mm sieve, weigh 0.5g of the sieved soil (fresh weight), add 5mL of K with a concentration of 0.5mol / L 2 SO 4 Aqueous solution, shake and extract at room temperature for 1h, pass through a 0.45μm filter membrane, and take the filtrate as soil solution; divide the soil solution into four groups for the determination of ammonium nitrogen, nitrate nitrogen, nitrite nitrogen and total dissolved nitrogen When measuring, the volume of every part of the soil solution is 1mL.

[0088] (1) Establishment of ammonium nitrogen concentration-absorbance standard curve

[0089] Preparation of oxidizing agent: measure 65mL of sodium hypochlorite solution (available chlorine content > 5.3%) and dissolve it in 1000mL of water; under stirring conditions, slowly add 0.025g of sodium chloroisocyanurate and let stand overnight to obtain the oxidizing agent.

[0090] Preparation of color developer: Weigh 144 g of sodium sal...

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Abstract

The invention belongs to the technical field of nitrogen content determination, and provides a determination method for ammonium nitrogen, nitric nitrogen, nitrite nitrogen and total dissolved nitrogen in samples. The method provided by the invention has the advantages that during determination of the ammonium nitrogen, a new oxidant (sodium hypochlorite and sodium chlorinated isocyanurate) and color developer (salicylic acid) system is adopted, the ammonium nitrogen is oxidized into monochloramine under the action of an oxidant, indophenol is obtained from the obtained monochloramine and a color developer, and the ultraviolet absorbance at a wavelength of 660nm is determined for measurement; a new first Griess reagent (N-(1-naphthyl)ethylenediamine dihydrochloride) and second Griess reagent (sulfanilamide and vanadium chloride) system is adopted for measurement of the nitric nitrogen, the nitrite nitrogen and the total dissolved nitrogen, so that low detection limits and high sensitivity are achieved; and the color development reaction is stable in speed and uniform, so as to be carried out on a 96-well plate.

Description

technical field [0001] The invention relates to the technical field of nitrogen content detection, in particular to a method for measuring ammonium nitrogen, nitrate nitrogen, nitrite nitrogen and total dissolved nitrogen in a sample. Background technique [0002] The method for measuring nitrogen content in soil in the prior art mainly relies on a common ultraviolet-visible spectrophotometer, which can only measure a limited number of samples each time. Moreover, limited by the speed and uniformity of the reaction during the determination, it cannot be realized on a 96-well plate (microplate reader). In addition, relying solely on the ordinary UV-Vis spectrophotometer method for measurement, the detection limit and accuracy need to be improved. Contents of the invention [0003] In view of this, the object of the present invention is to provide a kind of assay method of ammonium nitrogen, nitrate nitrogen, nitrite nitrogen and total dissolved nitrogen in a sample, assay ...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/33
CPCG01N21/78G01N21/33
Inventor 吴星贾永锋
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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