Symbiotic culture method for promoting development of cymbidium longibracteatum embryos in container

Abstract

The invention discloses a symbiotic culture method for promoting development of cymbidium longibracteatum embryos in a container, and belongs to the fields of applied microbiology and plant nutriology. The method comprises the steps that mycorrhiza of cymbidium goeringii var.longibracteatum is sheared for surface disinfection and inactivation of epiphytic bacteria, and the mycorrhiza is fully grinded to obtain cymbidium longibracteatum mycorrhiza homogenate is obtained; orchid soil, moss and a proper amount of the prepared cymbidium longibracteatum mycorrhiza homogenate are prepared into a symbiotic matrix, and aseptic cymbidium longibracteatum seeds are inoculated into the symbiotic matrix for culture, so that the development of the cymbidium longibracteatum embryos is promoted, and a symbiotic system is established; meanwhile, a control experiment is set; finally, microscopic examination is conducted on the symbiotic system to inspect the effectiveness of the cymbidium longibracteatum mycorrhiza homogenate to the growth and development of the cymbidium longibracteatum embryos. According to the technology, the culture method is simple, and a new way is developed for promoting re-introduction and wild large-scale growth of cymbidium longibracteatum.

Description

technical field [0001] The present invention relates to obtaining a kind of symbiosis culture method that promotes spring sword germ embryo development in a kind of container, specifically relates to a kind of wild spring sword ( Cymbidium goeringii var .longibracteatum ) mycorrhizae are ground into a homogenate, and then the aseptic spring sword seeds and the homogenate are symbiotically cultivated to promote the symbiotic culture method of the spring sword seed embryo development. Background technique [0002] Spring Sword is a unique orchid species in Southwest my country. Spring sword seeds are as small as dust and do not contain endosperm. Under normal circumstances, it is difficult to germinate, and they mostly rely on the symbiosis with endogenous beneficial fungi to germinate. At present, about the germination of spring sword seeds, most of them adopt non-symbiotic germination in the artificial medium under sterile conditions. The germination time of non-symbiotic...

AI Technical Summary

Problems solved by technology

At present, about the germination of spring sword seeds, most of them adopt non-symbiotic germination in the artificial medium under sterile conditions. The germination time of non-symbiotic germination is longer and when the seedlings obtained are reintroduced in the field, the seedlings are transplanted to the natural environment. The survival rate in the medium is low, and subsequent growth is severely limited due to the inability to establish a symbiotic relationship with fungi in nature