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Analytical method for detecting organophosphorus pesticide by ratio-dependent fluorescence sensor based on manganese dioxide nanosheet response

An organophosphorus pesticide and fluorescence sensor technology, which is applied in fluorescence/phosphorescence, material analysis by optical means, and material analysis, etc., can solve problems such as single response signal interference, and achieve low cost of raw materials, short experimental period and simple principle. Effect

Active Publication Date: 2019-10-11
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most fluorescence assays focus on turn-off or turn-on for detection, and a single response signal is disturbed by various experimental factors

Method used

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  • Analytical method for detecting organophosphorus pesticide by ratio-dependent fluorescence sensor based on manganese dioxide nanosheet response
  • Analytical method for detecting organophosphorus pesticide by ratio-dependent fluorescence sensor based on manganese dioxide nanosheet response
  • Analytical method for detecting organophosphorus pesticide by ratio-dependent fluorescence sensor based on manganese dioxide nanosheet response

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: Manganese dioxide nanosheet (MnO 2 NS) synthesis

[0035] 10mL of 3wt% hydrogen peroxide was mixed with 10mL of 1.2M tetramethylammonium hydroxide solution and quickly added 10mL of 0.3M MnCl within 15 seconds 2 solution, the solution immediately turned dark brown, and then the suspension was vigorously stirred at room temperature for 12 h, and the resulting solution was centrifuged in a centrifuge for 20 minutes (2000 rpm), then washed three times with water and methanol, and dried at 60 ° C. Dry in an oven to obtain massive manganese dioxide. Take by weighing 10mg massive manganese dioxide and dissolve in 10mL ultrapure water and ultrasonic 12h, manganese dioxide is completely dispersed, the dispersion is centrifuged in a centrifuge for 30 minutes (2000rpm), and the supernatant is retained for the next experiment.

[0036] image 3 A shows the synthesized MnO 2 TEM images of NS, from image 3 It can be seen from A that it has a two-dimensional shee...

Embodiment 2 2

[0037] Embodiment 2 Manganese dioxide nanosheets (MnO 2 NS) synthesis

[0038] Mix 10mL of 2wt% hydrogen peroxide with 10mL of 1.1M tetramethylammonium hydroxide solution and quickly add 10mL of 0.2M MnCl within 30 seconds 2 solution, the solution immediately turned dark brown, and then the suspension was vigorously stirred at room temperature for 12 h, and the resulting solution was centrifuged in a centrifuge for 20 minutes (2000 rpm), then washed three times with water and methanol, and dried at 60 ° C. Dry in an oven to obtain massive manganese dioxide. Take by weighing 10mg massive manganese dioxide and dissolve in 10mL ultrapure water and ultrasonic 12h, manganese dioxide is completely dispersed, the dispersion is centrifuged in a centrifuge for 30 minutes (2000rpm), and the supernatant is retained for the next experiment.

Embodiment 3

[0039] Example 3 acetylcholinesterase induces MnO 2 Nanosheets (MnO 2 NS) decomposition and fluorescence changes of SC and AR

[0040] 0.5 U / mL AChE (50 μL) and 5 mM ATCh (20 μL) were mixed with 30 μL PBS buffer. After incubating at 37°C for 30 minutes, 30 μL of the MnO prepared in Example 1 was added 2 Nanosheet solution (0.5 mg / mL) and 54 μL PB buffer. The resulting mixture was incubated at room temperature for 30 minutes. Next, add 6 μL of SC (50 μM) and 10 μL of AR (50 μM) to the above mixture and make sure the final volume is 200 μL. Fluorescence spectra of different samples were recorded under excitation at 380 nm and 560 nm, respectively, at room temperature. figure 2 A is the fluorescence spectrum of SC and figure 2 B is the fluorescence spectrum of AR, ATCh / MnO2 NS / SC / AR (a), ATCh / AChE / MnO2 NS / SC / AR (c).

[0041] 0.5 U / mL AChE (50 μL) and 5 mM ATCh (20 μL) were mixed with 30 μL PBS buffer. After incubating at 37°C for 30 minutes, 30 μL of the MnO prepared i...

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Abstract

The invention discloses an analytical method for detecting an organophosphorus pesticide by a ratio-dependent fluorescence sensor based on manganese dioxide nanosheet response. The analytical method comprises the following steps: mixing and reacting the organophosphorus pesticide with acetylcholin esterase to obtain a mixed solution, then adding an acetylcholine chloride solution and a PBS buffersolution into the mixed solution for a reaction so as to obtain a solution, adding a manganese dioxide nanosheet soluktion and the PBS buffer solution into the solution for a reaction at room temperature to obtain a mixture, finally adding Scopoletin and Amplex Red into the mixture for a reaction in a dark environment, and recording a solution fluorescence spectrum to obtain the concentration of the organophosphorus pesticide through fluorescence intensity. Without the need of the detection of expensive precise instruments, the detection method is simplified, the detection cost of the organophosphorus pesticide is greatly reduced, and the analytical method has the advantages of low operation cost, quick, simple and convenient detection, high selectivity and the like.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to an analysis method for detecting organophosphorus pesticides by a ratiometric fluorescent sensor based on the response of manganese dioxide nanosheets. Background technique [0002] As common pesticides, organophosphorus pesticides (OPs) are widely used in agricultural production. OPs play a huge role in improving crop yield and controlling pests and diseases. However, excessive use of OPs can lead to different levels of residues in food, water and the environment. OPs can inhibit the activity of acetylcholinesterase (AChE), seriously affecting human health. Acetylcholinesterase (AChE) is an acetylcholine hydrolase mainly present in the central nervous system of humans and animals. Its basic function is to catalyze the hydrolysis of the neurotransmitter acetylcholine, leading to the termination of nerve impulse transmission, thereby maintaining the normal physiological fun...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 卫伟姚田田卫敏刘松琴
Owner SOUTHEAST UNIV
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