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Liquid microbial agent containing polyglutamic acid and preparation method thereof

A technology of liquid bacterial agent and polyglutamic acid, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low effective bacteria content and the effectiveness of easily inactivated products, and achieve high charge density , Promote photosynthesis, improve vegetative growth and reproductive growth

Pending Publication Date: 2019-10-11
轩凯生物科技(滁州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Purpose of the invention: In order to solve the problems of low effective bacteria content, easy inactivation during transportation and poor product effectiveness of existing microbial liquid bacterial agents, the present invention provides a microorganism suitable for aquaculture with multiple functions such as water purification and production increase Liquid bacteria

Method used

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  • Liquid microbial agent containing polyglutamic acid and preparation method thereof
  • Liquid microbial agent containing polyglutamic acid and preparation method thereof
  • Liquid microbial agent containing polyglutamic acid and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The fermentation of embodiment 1 growth-promoting bacteria

[0030] Prepare the growth-promoting bacteria liquid according to the following steps:

[0031] Bacillus amyloliquefaciens CCTCC NO:M 2016266 and Bacillus subtilis CCTCC NO:M 2016264 stored on a slant at 4°C were activated, respectively inoculated on eggplant-shaped culture flask medium (ie activation medium), and cultured at 32°C for 18 hours. Transfer the activated strains to the fermentation medium, culture at 30°C for 24h, place the fermented liquid in a cold storage at 4°C for 48h at low temperature, and measure by plate coating, the number of viable bacteria in each microbial fermentation liquid can reach 8 ×10 9 CFU / mL or more, the two microbial fermentation broths are mixed according to the volume ratio of 1:1 to obtain the growth-promoting bacteria broth.

[0032] The medium in the described eggplant-shaped culture bottle is LB medium, and the fermentation medium component is: glucose 50g / L, peptone ...

Embodiment 2

[0034] The preparation of embodiment 2 compound microbial liquid inoculum

[0035] Prepare a compound microbial liquid bacterial agent containing growth-promoting bacteria liquid, polyglutamic acid, minerals, biological organic matter, chlorophyll, and vitamins as follows:

[0036] (1) Polyglutamic acid was prepared by fermentation (reference Xu Z, Feng X, Dan Z, et al. Enhanced poly(γ-glutamic acid) fermentation by Bacillus subtilis, NX-2 immobilized in anaerobic plant fibrous-bed bioreactor[J ].Bioresour Technol, 2014,155:8-14), the polyglutamic acid content of the fermented liquid is 15% after concentration;

[0037] (2) Minerals are purchased from the market;

[0038] (3) The molasses is purchased by the sugar factory;

[0039] (4) Chlorophyll is purchased from the market;

[0040] (5) Vitamins are purchased from the market;

[0041] (6) Mix the composite growth-promoting bacteria liquid obtained in Example 1 with the polyglutamic acid fermentation concentrate, mineral...

Embodiment 3

[0042] Embodiment 3 Composite microbial liquid inoculum can be kept open for up to 18 months

[0043] The bacterial agent used in the present embodiment is as follows:

[0044] (1) Dilute the mixed microbial agent prepared in Example 1 to an effective viable count of 7.6 × 10 9 CFU / mL, as bacterial agent 1.

[0045] (2) the mixed bacterial agent prepared by embodiment 1 is mixed evenly with mineral matter, molasses, chlorophyll, vitamin in proportion, so that the mineral matter content is 1.5%, the organic matter content is 65%, the chlorophyll content is 1.5%, and the vitamin content is 1.5%. 1%, the effective number of viable bacteria is 7.6×10 9 CFU / mL, as bacterial agent 2.

[0046] (3) Mix the mixed bacterial agent prepared in Example 1 with polyglutamic acid, molasses, chlorophyll, and vitamins in proportion, so that the polyglutamic acid content is 3.2%, the organic matter content is 65%, and the chlorophyll content is 1.5%. , the vitamin content is 1%, and the effe...

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Abstract

The invention discloses a liquid microbial agent containing polyglutamic acid and a preparation method thereof. The liquid microbial agent is prepared from 1-20 parts of a growth-promoting bacterium liquid, 1-20 parts of polyglutamic acid, 1-20 parts of mineral substances, 50-70 parts of bioorganic matter, 1-5 parts of chlorophyll and 1-5 parts of vitamin. The composite liquid microbial agent hasthe advantages of being high in water-soluble speed and content of viable organisms, and capable of inhibiting pathogenic microorganisms, flocculating harmful substances in a water body to purify water quality, chelating heavy metal ions in the water body to alleviate the toxicity of the heavy metal ions, and promoting photosynthesis of algae to provide aquatic animals with rich nutrition, and hasmultiple functions of water purification, production increase and the like.

Description

technical field [0001] The invention relates to the technical field of microbial bacterial agents, in particular to a microbial liquid bacterial agent containing polyglutamic acid suitable for aquaculture and a preparation method thereof. Background technique [0002] In recent years, intensive aquaculture has developed rapidly at home and abroad, and a large amount of bait enters the environment in the form of fish metabolites and leftover bait, causing pollution of water bodies and pond sediments. The ecological function of the aquaculture waters is degraded, and the diseases are becoming more and more serious, which has caused huge economic losses to the aquaculture industry. [0003] Common methods for water pollution treatment include chemical treatment, physical treatment and biological treatment, among which biological treatment is increasingly used. However, most of the existing biological treatment products used to treat water pollution have low effectiveness, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34A01K63/04C12R1/07C12R1/125C02F103/20
CPCC12N1/20C02F3/34A01K63/04C02F2103/20
Inventor 徐虹冯小海许宗奇
Owner 轩凯生物科技(滁州)有限公司
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