Novel multi-layer adherent cell culture container structure

Abstract

The invention relates to the field of culture containers, in particular to a novel multi-layer adherent cell culture container structure. The novel multi-layer adherent cell culture container structure comprises a multi-layer cell factory main body, a bayonet cover and a breathable cover, wherein the interior of the multi-layer cell factory main body is provided with a plurality of groups of multi-layer culture surfaces in an equally-spaced mode from top to bottom; multi-layer culture surface fixed grooves are formed in the multi-layer cell factory main body at the position close to the multi-layer culture surfaces; extension tube openings are formed in the side, close to a base surface and a back surface, of the upper end of the multi-layer cell factory main body respectively; the bayonet cover is fixedly mounted at the upper end of the extension tube opening in the side, close to the base surface, of the upper end of the multi-layer cell factory main body; the breathable cover is fixedly mounted at the upper end of the extension tube opening in the side, close to the back face, of the upper end of the multi-layer cell factory main body; the interior of the breathable cover is provided with an extension tube; and the exterior of the extension tube is provided with a breathable film. Through the application of the breathable cover, the container can be used for flooded culture, the storey height is reduced, the culture area is greatly increased under the same height, and the utilization rate of space is promoted.

Description

technical field [0001] The invention relates to the field of culture containers, in particular to a novel multi-layer adherent cell culture container structure. Background technique [0002] Since conventional multi-layer culture bottles are mostly various deformation forms of T175 bottles, such as multi-layer bottles, cellstack, etc., the culture method is mostly layered culture, and there must be a certain height between layers to meet the demand for ventilation. Also reaching a certain amount of air flow can greatly shorten the height between layers, save space and increase the cultivation area. [0003] Conventional multi-layer culture flasks cannot satisfy the cultivation of a large number of adherent cells in a full liquid culture container, and cannot meet the gas permeability requirements for cell growth. Therefore, a new type of multi-layer adherent cell culture container structure is needed to improve the above problems . Contents of the invention [0004] The ...

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Problems solved by technology

[0003] Conventional multi-layer culture flasks cannot satisfy the cultivation of a large number of adherent cells in a full liquid culture container, and cannot meet the gas permeability requirements for cell growth. Therefore, a new type of multi-layer adherent cell culture container structure is needed to improve the above problems

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