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Myocardial-cell-derived exosome based on Tongxinluo pretreatment and preparation method thereof

A technique for cardiomyocytes and exosomes, applied in the field of cardiomyocyte-derived exosomes and their preparation

Active Publication Date: 2019-09-20
FUWAI HOSPITAL CHINESE ACAD OF MEDICAL SCI & PEKING UNION MEDICAL COLLEGE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is currently no effective drug that can reduce myocardial ischemia-reperfusion injury (Heusch, G. (2015). Molecular basis of cardioprotection: signal transduction in ischemic pre-, post-, and remoteconditioning. Circ. Res. 116, 674–699)

Method used

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  • Myocardial-cell-derived exosome based on Tongxinluo pretreatment and preparation method thereof
  • Myocardial-cell-derived exosome based on Tongxinluo pretreatment and preparation method thereof
  • Myocardial-cell-derived exosome based on Tongxinluo pretreatment and preparation method thereof

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Experimental program
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Embodiment 1

[0039] Preparation method of cardiomyocyte-derived exosomes:

[0040] Human cardiomyocytes (CM) were cultured and subcultured to the 5th to 7th generation for future use. TXL was dissolved in sugar-free and serum-free DMEM medium (Life Technologies, USA), and after pretreating CM for 1 hour, the cell culture medium was replaced with TXL-free, sugar-free and serum-free DMEM medium. Put the CM in the hypoxia box containing the anaerobic indicator strip, put it into the anaerobic bag, and quickly close the hypoxia box; place the hypoxia box at 37°C, 5% CO 2 In the cell culture incubator, hypoxia was treated for 18 hours. After 18 hours of hypoxia, open the lid of the culture bottle (plate) in a sterile operating table, shake the medium to promote rapid reoxygenation of the cells, then cover the lid and put it back in the incubator for reoxygenation for 2 hours [Note: This hypoxia device It takes 2.5 hours to completely remove the oxygen in the hypoxia box, and the box has reach...

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Abstract

The invention provides a myocardial-cell-derived exosome based on Tongxinluo pretreatment and a preparation method thereof. The preparation method comprises: pretreating myocardial cells with Tongxinluo, subjecting the pretreated myocardial cells to hypoxia / reoxygenation, and collecting an exosome secreted by the myocardial cells. The myocardial-cell-derived exosome effective in reducing the apoptosis of myocardial microvascular endothelial cells to alleviate myocardial Ischemia reperfusion injury can be prepared via the preparation method.

Description

technical field [0001] The present invention relates to a cardiomyocyte-derived exosome based on drug pretreatment and a preparation method thereof, in particular to a cardiomyocyte-derived exosome based on drug Tongxinluo pretreatment and a preparation method thereof. Background technique [0002] WHO statistics show that in 2016, cardiovascular disease was the leading cause of death in the world (about 17.6 million people, 32.2%), of which 9.48 million people died of ischemic heart disease (17.3%) (Collaborators GM. Global, regional, andnational under-5 mortality, adult mortality, age-specific mortality, and life expectancy, 1970–2016: a systematic analysis for the Global Burden of Disease Study 2016. The Lancet 2017; 390(10100):1084-1150; Collaborators GCOD. Global, regional, and national age-sex specific mortality for 264 causes of death, 1980–2016: a systematic analysis for the Global Burden of Disease Study 2016. The Lancet 2017; 390(10100):1151-1210). Acute myocardia...

Claims

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Application Information

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IPC IPC(8): C12N5/077A61K35/34A61P9/10A61P9/00
CPCA61K35/34A61P9/00A61P9/10C12N5/0657C12N2500/02C12N2500/76C12N2500/82C12N2509/10
Inventor 杨跃进陈桂浩黄沛森
Owner FUWAI HOSPITAL CHINESE ACAD OF MEDICAL SCI & PEKING UNION MEDICAL COLLEGE
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