Preparation method of Sauropus spatulifolius total flavonoids

Abstract

The invention discloses a preparation method of Sauropus spatulifolius total flavonoids. The preparation method includes: selecting the dried leaves of Sauropus spatulifolius, and using 95% ethanol to perform heating reflux extraction on the dried leaves to obtain Sauropus spatulifolius extract; subjecting the Sauropus spatulifolius extract to reduced pressure recycling, and concentrating until no alcohol smell exists to obtain Sauropus spatulifolius ethanol-extraction extractum; evenly mixing the Sauropus spatulifolius ethanol-extraction extractum with deinonized water, performing suspension filtration to obtain Sauropus spatulifolius ethanol-extraction supernate, extracting the Sauropus spatulifolius ethanol-extraction supernate with petroleum ether to obtain a Sauropus spatulifolius extraction water layer; subjecting the Sauropus spatulifolius extraction water layer to ODS reduced pressure column chromatography, and using a methanol-water system to perform gradient elution to obtain the Sauropus spatulifolius total flavonoids. The preparation method has the advantages that the method is simple, low in experiment material requirements and easy to operate; the prepared Sauropus spatulifolius total flavonoids has inhibitory activity to alpha-glucosidase, six flavonoid compounds in the Sauropus spatulifolius total flavonoids has inhibitory activity to alpha-glucosidase, and the Sauropus spatulifolius total flavonoids are safe to normal alveolar cells, applicable to the preparation of anti-diabetic medicine and capable of being used as the supplementary food of diabetics.

Description

technical field [0001] The invention specifically relates to a preparation method of total flavonoids from leaves of asparagus. Background technique [0002] Sauropus spatulifolius Beille (Sauropus spatulifolius Beille) is a plant of the Euphorbiaceae gecko, also known as dragon tongue leaf and dragon ear leaf. It is native to northern Vietnam and cultivated in the Malay Peninsula. In my country, it is mostly distributed in Fujian, Guangdong, Guangxi and other places. . Folk areas often use Asparagus leaves as dietary medicinal materials. Its taste is sweet, light, and flat in nature. It has the effect of clearing the lungs and removing heat, resolving phlegm and relieving cough. Asparagus leaves are rich in effective substances, but there are not many studies on this topic in the market at present. Some researchers have identified through chemical reactions that asparagus leaves may contain sugars, saponins, tannins, organic acids, alkaloids, coumarins, etc. Chemical compo...

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Problems solved by technology

However, the chemical constituents and medicinal substances of Asparagus leaves are not clear, and further research is still needed.

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