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Method for detecting inflammatory markers and kit for detecting inflammatory markers

A detection kit and detection method technology are applied in the detection of inflammatory markers and the field of inflammatory marker detection kits, which can solve the problems of narrow detection range of inflammatory markers, low sensitivity, and requirements for manual operation of climate conditions, etc. Stable test results, high accuracy and sensitivity, and easy operation

Pending Publication Date: 2019-09-13
SHENZHEN DYMIND BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to provide a detection method for inflammatory markers and a detection kit for inflammatory markers, which solves the problem that the detection range of inflammatory markers in the prior art is narrow, the sensitivity is not high, manual operation is required, and the requirements for weather conditions high problem

Method used

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  • Method for detecting inflammatory markers and kit for detecting inflammatory markers
  • Method for detecting inflammatory markers and kit for detecting inflammatory markers
  • Method for detecting inflammatory markers and kit for detecting inflammatory markers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0114] Example 1: Simultaneous detection of three markers SAA / PCT / CRP

[0115] 1. Prepare

[0116] 1.1 Select different codable magnetic fluorescent microspheres according to different projects

[0117] SAA→No.8 Microspheres

[0118] PCT→No.1 microsphere

[0119] CRP → No. 15 microspheres;

[0120] 1.2 Take 1 mg of the prepared No. 1 / 8 / 15 microspheres respectively, and wash them with MES buffer (50 mM) at pH 5.5;

[0121] 1.3 Use 0.1M pH6.2Na 2 HPO 4 The solution was activated and vortexed for 20s; after that, 100 μg of NHS and EDC were added respectively (NHS and EDC were dissolved in 50 mM MES buffer solution with pH 5.5, which is now used and prepared), vortexed for 20s, and room temperature for 30 minutes;

[0122] 1.4 Use a magnetic separation plate for magnetic separation, and absorb the supernatant; add 500 μL of MES buffer solution (50 mM) at pH 5.5, vortex the microspheres for 20 seconds, perform magnetic separation with a magnetic separation plate, and absorb t...

Embodiment 2

[0148] Example 2: Simultaneous detection of three markers PCT / SAA / CRP

[0149] 1. Prepare

[0150] 1.1 Select different codable magnetic fluorescent microspheres according to different projects

[0151] PCT→16 microspheres

[0152] SAA→24 microspheres

[0153] CRP → No. 26 microspheres;

[0154] 1.2 Take 1 mg of the prepared No. 16 / 24 / 26 microspheres respectively, and wash them with MES buffer (20 mM) at pH 5.0;

[0155] 1.3 Use 0.1M pH6.2Na 2 HPO 3 The solution was activated and vortexed for 20s; after that, 100 μg of NHS and EDC were added respectively (NHS and EDC were dissolved in 20 mM MES buffer with pH 5.0, and prepared immediately after use), vortexed for 20s, and 30 minutes at room temperature.

[0156] 1.4 Use a magnetic separation plate for magnetic separation, and absorb the supernatant; add 500 μL of MES buffer solution (20 mM) at pH 5.0, vortex the microspheres for 20 seconds, perform magnetic separation with a magnetic separation plate, and absorb the supern...

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Abstract

The invention relates to a method for detecting inflammatory markers and a kit for detecting inflammatory markers to solve the problems of narrow detection range, low sensitivity, manual operation andhigh requirements on climatic conditions for detecting inflammatory markers in the prior art. The method for detecting inflammatory markers and the kit for detecting inflammatory markers provided bythe invention have wider detection range, higher accuracy and sensitivity, can simultaneously detect SAA, PCT and CRP, and are convenient in operation and stable in detection result.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnosis, and more specifically, relates to a method for detecting inflammatory markers and a kit for detecting inflammatory markers. Background technique [0002] Serum amyloid A (SAA), as an acute phase reaction protein, increases to varying degrees during bacterial, viral, and fungal infections. During an inflammatory response, IL-1 and TNF released by macrophages make IL-6 Increased secretion, which stimulates hepatocytes to secrete SAA. Within 4-6 hours, the concentration of SAA increased rapidly, up to 1000 times the original level. [0003] C-reactive protein (CRP) is an extremely sensitive indicator of acute phase response, and its concentration in plasma rises sharply when the body is infected or tissue is damaged. At the same time, CRP is directly involved in cardiovascular diseases such as inflammation and atherosclerosis, and is the most powerful predictor and risk factor for cardi...

Claims

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Application Information

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IPC IPC(8): G01N33/533G01N33/543
CPCG01N33/533G01N33/5434
Inventor 李春晖王业红张文琪张志花
Owner SHENZHEN DYMIND BIOTECH
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