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MED12 gene mutation detecting kit and application thereof

A kit and gene technology, applied in the field of medical testing

Active Publication Date: 2019-09-13
PEOPLES HOSPITAL PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no research report on MED12 mutation and its exact tumor-promoting effect in AML.

Method used

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  • MED12 gene mutation detecting kit and application thereof
  • MED12 gene mutation detecting kit and application thereof
  • MED12 gene mutation detecting kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] Embodiment 1, the kit for detecting MED12 gene mutation and its detection method

[0099] 1. Kit for detecting MED12 gene mutation

[0100] 1. Probe sequence

[0101] The kit for detecting MED12 gene mutation of the present invention includes 48 probe sequences, and the names and specific sequences of the 48 probe sequences are shown in Table 1. The 48 probes of the present invention can cover the entire coding sequence of the MED12 gene.

[0102] Probe sequence in table 1, detection kit of the present invention

[0103]

[0104]

[0105] 2. Target area detection principle

[0106] The genomic DNA of the sample to be tested was randomly broken into fragments with a length of 200-300bp by a Covaris ultrasonic breaker, and after end repair and A-tailing, adapters were connected to both ends of the fragments to prepare a DNA library. The probe in step 1 was biotin-labeled, and then the DNA library with a specific index was hybridized with the biotin-labeled probe...

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Abstract

The present invention discloses a MED12 gene mutation detecting kit and an application thereof. The kit comprises probe sets for detecting MED12 gene mutations and the probe sets consist of a probe 1to a probe 48. The kit can be used for detecting MED12 somatic mutations and germline mutations at a tumor genome level, can also be used for assistant diagnosis and minimal residual disease monitoring and prognosis evaluation, etc. of patients with hematological tumors, especially patients with acute myeloid leukemia. Besides, the kit is simple, sensitive and accurate in detection method and plays an important role in the field of medical detection

Description

technical field [0001] The invention belongs to the technical field of medical detection, and in particular relates to a MED12 gene mutation detection kit and application thereof. Background technique [0002] Acute myeloid leukemia (AML) is a malignant clonal disease originating from hematopoietic stem cells with high molecular biology and clinical heterogeneity. The development of molecular diagnostic technology has played a huge role in the precise classification and individualized treatment of AML, but despite this, the clinical prognosis of AML patients still shows huge heterogeneity, and recurrence is still common among clinicians and AML patients. One of the challenges we face, and the discovery of new biomarkers is the only way to realize the individualized diagnosis and treatment of AML. [0003] MED12 (mediator complex subunit 12) is located on the X sex chromosome and encodes the mediator complex subunit 12 protein. It forms a four-subunit kinase module together ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12Q1/6858C12N15/11
CPCC12Q1/6886C12Q1/6858C12Q2600/156C12Q2535/122
Inventor 阮国瑞黄晓军周亚兰吴利新王子龙
Owner PEOPLES HOSPITAL PEKING UNIV
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