Pesticide residue detection powder and preparation method, cuvette and detection method

Abstract

The invention discloses a preparation method of pesticide residue detection powder. The method comprises the steps of: adding acetylcholin esterase solution, a color development reagent and a protective agent into a cuvette, and placing the cuvette into a freezing drying box; after enabling solution in the cuvette to be frozen, sublimated and dried by a freezing drying process, forming powder; andcarrying out sealing storage on the cuvette containing the powder. After the preparation method of the pesticide residue detection powder, the powder, the cuvette and a detection method which are provided by the invention are adopted, the operation steps of pesticide residue detection can be simplified so as to facilitate standardization and rapidness of pesticide residue detection, detection onfruit and vegetable pesticide residues can be automatically executed by a detection instrument so as to enable primary detection personnel to be separated from the heavy pesticide residue detection task, and meanwhile, the automatic operation of an instrument avoids the non-standard problem of the detection personnel on the detection operation.

Description

technical field [0001] The invention relates to a pesticide residue detection powder and a preparation method, a corresponding cuvette and a detection method. Background technique [0002] In the field of rapid detection of food safety, the rapid detection of pesticide residues accounts for more than 60% of the total detection volume. In the rapid detection of pesticide residues, the current mainstream detection methods are based on GB / T 5009.199-2003 "Rapid Detection of Organophosphorus and Carbamate Pesticide Residues in Vegetables" and KJ 201710 "Trichlorfon and Profenofos in Vegetables" "Rapid detection of residues of methomyl, carbofuran and dichlorvos" for the detection of organophosphorus and carbamate pesticide residues. The basic detection principle of the two is that under certain conditions, organophosphorus and carbamate pesticides can inhibit the normal function of cholinesterase, and the inhibition rate is positively correlated with the concentration of the pe...

AI Technical Summary

Problems solved by technology

[0004] However, in the process of applying this technology to various agricultural technology extension centers and food safety risk monitoring in food production and circulation links, due to the lack of technical skills of grass-roots inspectors, the problem of non-standard detection operations occurred in the process of pesticide residue detection ; At the same time, due to the large number of inspection batches of fruit and vegetable pesticide residues in the food production and circulation link, the inspection time is long and the inspectors have heavy tasks, which further affects the standardization and reliability of the inspectors in the inspection of vegetable pesticide residues.

At present, there is no relevant improvement and optimization in the detection of pesticide residues in fruits and vegetables and the application of pesticide residue detection reagents based on the above two detection standards.

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