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Method for preparing myocardial patch and application of myocardial patch

A technology of patch and myocardial patch, which is applied in the field of preparation of myocardial patch, which can solve the problems of uneven distribution of cells on the patch, side effects, and few active cells, achieve good market prospects and social public welfare value, improve proliferation efficiency, and restore activity Effect

Active Publication Date: 2019-09-06
嘉兴莱普晟医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, according to different seed cell types and their biological characteristics, tissue engineering researchers have developed a variety of cell sheet construction methods, such as temperature-sensitive method, nano-magnetic particle method, etc. Materials are usually inoculated uniformly, but during the process of cell growth and proliferation, due to the different states of different cells, their proliferation is not uniform, which leads to uneven distribution of cells on the patch, and at the same time, causes myocardial infarction. The shape and size of the myocardial infarction site on the myocardium are also different due to different factors
Due to the inhomogeneous distribution of cells in the myocardial patch prepared by the prior art, there may be fewer active cells in the myocardial infarction site, resulting in a poor therapeutic effect. Covering, usually requires a larger patch, which leads to many active cells actually covering the normal cells, resulting in unnecessary waste and unknown side effects

Method used

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  • Method for preparing myocardial patch and application of myocardial patch
  • Method for preparing myocardial patch and application of myocardial patch
  • Method for preparing myocardial patch and application of myocardial patch

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1 is used to prepare the preparation of the composition MA-Gelzan-Gly-Go-Ito-DA of myocardial patch

[0042] 1. Preparation of MA-Gelzan-Gly blend

[0043] Weigh 2 g of gelatin (Sigma), put it into 20 ml of PBS solution, heat it in a water bath at 60 ° C, stir it by magnetic force for 40 min, and dissolve it fully to obtain a 10 w / v% gelatin solution; dissolve 0.8 ml of glycerin in 20 ml of distilled water, mix Evenly, to obtain a 4v / v% glycerol solution; Weigh 1g of G3251 (a plant gel produced by Phytotechlab, CAS: 71010-52-1), put it into 10ml of PBS solution, heat it in a water bath at 60°C, and Stir for 30 minutes, fully dissolve, and obtain a 10w / v% G3251 solution; mix the above-mentioned 10w / v% gelatin solution, 4w / v% glycerin solution, and 10w / v% G3251 solution in a volume ratio of 1:1:1 Mix, heat in a water bath at 60° C., and magnetically stir for 1 h to obtain a MA-Gelzan-Gly blend.

[0044] 2. Preparation of modified MA-Gelzan-Gly hydrogel

[00...

Embodiment 2

[0053] The influence of the proportioning of embodiment 2 different gelatin, glycerin, gellan gum on the mechanical properties of MA-Gelzan-Gly-Go-Ito-DA myocardial patch material

[0054] 1. Preparation of different types of myocardial patch materials

[0055] In order to explore the influence of different ratios of gelatin, glycerin, and gellan gum on the mechanical properties of the MA-Gelzan-Gly-Go-Ito-DA myocardial patch material, the embodiment designed 3 different gelatin, glycerin, and gellan gum, Specifically as described in Table 1:

[0056] Table 1 Myocardial patch materials prepared in different volume ratios of gelatin, glycerin, and gellan gum

[0057] Myocardial patch material model Volume ratio MA-Gelzan-Gly-Go-Ito-DA-a 1:1:1 MA-Gelzan-Gly-Go-Ito-DA-b 1:1.2:1 MA-Gelzan-Gly-Go-Ito-DA-c 1.2:1:1.5

[0058] The preparation method of MA-Gelzan-Gly-Go-Ito-DA myocardial patch material is as described in Example 1, 10w / v% gelatin sol...

Embodiment 3

[0064] The influence of the graphene oxide of embodiment 3 different content on MA-Gelzan-Gly-Go-Ito-DA myocardial patch material mechanical property and conductivity

[0065] 1. Preparation of different types of myocardial patch materials

[0066] In order to explore the effect of graphene oxide on the mechanical properties and electrical conductivity of MA-Gelzan-Gly-Go-Ito-DA myocardial patch material, when the ratio of gelatin, glycerin and gellan gum was 1.2:1:1.5, a 4 different graphene oxide contents, specifically as described in Table 2:

[0067] Table 2 Myocardial patch materials prepared by different contents of graphene oxide

[0068] Myocardial patch material model The ratio of MA-Gelzan-Gly hydrogel solution to graphene oxide solution MA-Gelzan-Gly-Go-Ito-DA-c 4:1 MA-Gelzan-Gly-Go-Ito-DA-d 4:1.5 MA-Gelzan-Gly-Go-Ito-DA-e 4:2 MA-Gelzan-Gly-Go-Ito-DA-f 4:2.5

[0069] The preparation method of MA-Gelzan-Gly-Go-Ito-DA myocardi...

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Abstract

The invention provides a method for preparing a myocardial patch. The method includes the steps: (1) preparing myocardial patch materials; (2) controlling uniform arrangement of cells in the patch materials by dielectrophoresis. The cells are controlled by the dielectrophoresis and uniformly distributed in the myocardial patch materials, and the proliferation efficiency of the cells in the materials is improved. Besides, cell arrangement on the patch materials can be adjusted according to characters of a myocardial infarction portion, the myocardial infarction portion can be completely coveredby the smaller patch, and myocardial infarction can be more effectively treated. The industrially applicable myocardial patch with excellent performances and capable of more effectively reactivatingthe myocardial infarction portion is provided for the prior art, and the myocardial patch has good market prospects and social benefit values.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, and in particular relates to a method for preparing a myocardial patch and an application thereof. Background technique [0002] Ischemic heart disease, as one of the main health problems in our country, has brought a heavy economic burden to our country and other countries in the world. Cardiomyocytes are terminally differentiated cells that are difficult to regenerate after necrosis. Although the current drugs, interventional and surgical treatment of ischemic heart disease can effectively improve myocardial blood supply and save dying myocardium, they have no repair effect on non-functional myocardium that has caused death. Severe cardiac arrhythmias, heart failure, and cardiac arrest can be caused by massive myocardial death. For the treatment of advanced heart disease, the more effective method is myocardial transplantation. However, the number of donors for myocardial transplantatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/22
CPCA61L27/222A61L27/3804A61L27/3834A61L27/3839A61L27/3895A61L2430/20
Inventor 李建辉郑俊辛立明杨子江黄光涛毕会民胡刚良
Owner 嘉兴莱普晟医疗科技有限公司
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