Confocal imaging device and confocal imaging method

Abstract

The invention discloses a confocal imaging device and a confocal imaging method. The embodiment of the invention adopts the aperture imaging method to solve the complicated problem in the scanning illumination process, and sets an adjustable plane reflector array, and utilizes the adjustment of the plane reflector array It overcomes the problem that confocal illumination cannot be shifted, realizes confocal imaging in application scenarios such as underwater, and expands the scope of application scenarios.

Description

technical field [0001] The invention relates to the field of computer technology, in particular to a confocal imaging device and a confocal imaging method. Background technique [0002] Confocal means that the light path including excitation and emission is brought into focus at two locations. During confocal imaging, excitation light is focused on the sample surface, while emission light is focused on the illumination pinhole. This pinhole limits the focus depth of the optical path on the sample surface, effectively preventing background noise interference from impurity signals, thereby reducing the intensity of the background signal and improving the imaging quality of the sample. The confocal imaging method was first applied to confocal microscopy, which mainly uses focused mode illumination and simultaneous imaging to create three-dimensional cross-sections of biological specimens. It is an optical imaging method that uses light focusing illumination and illumination p...

AI Technical Summary

Problems solved by technology

[0004] However, both of the above two methods for confocal imaging have disadvantages: the confocal scanning method has a small imaging range due to the small apertures of the illumination pinhole and the detection pinhole, and the scanning method is used to complete the confocal illumination and imaging. The process is cumbersome and slow, and once applied to a slightly larger scene, it will consume a lot of time and energy

The confocal encoding method overcomes the shortcomings of the confocal scanning method, but the plane mirror array is fixed and cannot be adjusted, which is not simple and flexible enough, which limits the scope of confocal imaging application scenarios

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