Brasenia schreberi in vitro gum polysaccharide fractional purification method capable of improving blood glucose lowering activity

A technology for fractional purification and hypoglycemia, applied in the field of plant extraction, can solve problems such as the influence of biological activity, and achieve the effects of fast separation speed, high separation rate and good classification effect.

Inactive Publication Date: 2019-09-03
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Liu Cuili et al. conducted animal experiments on the obtained Brasenia polysaccharides in mice, and the results showed that it has a significant hypoglycemic effect, but it is not yet clear whether polysaccharides with different molecular weights in Brasenia have an impact on biological activity.

Method used

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  • Brasenia schreberi in vitro gum polysaccharide fractional purification method capable of improving blood glucose lowering activity
  • Brasenia schreberi in vitro gum polysaccharide fractional purification method capable of improving blood glucose lowering activity
  • Brasenia schreberi in vitro gum polysaccharide fractional purification method capable of improving blood glucose lowering activity

Examples

Experimental program
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Embodiment 1

[0049] (1) Take a water shield sample, squeeze and filter with gauze to obtain water shield in vitro glue from the water shield sample, and remove excess water;

[0050] (2) Store water shield in vitro glue at -18°C, take it out and put it in a beaker to thaw for 2 hours, then take 100g of water shield in vitro glue in a beaker, dissolve it in 0.1mol / L NaOH solution, and the ratio of solid to liquid is 1 : 10 (g / mL), put it in a water bath at 70°C, and extract polysaccharides in a water bath for 1 hour; after extraction, cool to room temperature, filter the obtained extract to remove impurities to obtain a filtrate, adjust the pH to 7 with phosphoric acid solution , to obtain the crude polysaccharide of water shield in vitro;

[0051](3) Pass the crude polysaccharide of water shield in vitro glue through ultrafiltration membrane equipment, pass the crude polysaccharide of water shield in vitro glue through filter membranes with membrane pore diameters of 100kDa, 50kDa and 10kD...

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Abstract

The invention discloses a Brasenia schreberi in vitro gum polysaccharide fractional purification method capable of improving blood glucose lowering activity. The method comprises: taking a Brasenia schreberi sample, and separating the Brasenia schreberi sample to obtain a Brasenia schreberi in vitro gum; dissolving the Brasenia schreberi in vitro gum in an alkaline aqueous solution, and carrying out water bath heating leaching; after completing the leaching, filtering to obtain a filtrate; adjusting the pH value of the filtrate to a neutral pH value so as to obtain a Brasenia schreberi in vitro gum crude polysaccharide; and carrying out ultra-filtration on the Brasenia schreberi in vitro gum crude polysaccharide sequentially with filtration membranes with pore sizes of 100 kDa, 50 kDa and10 kDa to obtain three Brasenia schreberi in vitro gum polysaccharide components respectively with molecular weights of 100-50 kDa, 50-10 kDa and less than 10 kDa. According to the present invention,the Brasenia schreberi in vitro gum polysaccharide is subjected to fractional ultra-filtration treatment by using the ultra-filtration membrane separation technology so as to obtain the three Braseniaschreberi in vitro gum polysaccharide components respectively with molecular weights of 100-50 kDa, 50-10 kDa and less than 10 kDa, wherein the Brasenia schreberi in vitro gum polysaccharide component with the molecular weight of 100-50 kDa has high anti-oxidation property and in vitro enzyme activity inhibition ability.

Description

technical field [0001] The invention relates to the technical field of plant extraction, in particular to a method for fractional purification of water shield in vitro gum polysaccharides with improved hypoglycemic activity. Background technique [0002] Brasenia schreberi (Brasenia schreberi), commonly known as water sunflower, horseshoe grass, brocade, water lotus leaves, lake vegetables, etc., Brasenia schreberi is a perennial freshwater aquatic herb of the primitive perianth subclass Nymphaeaceae Brasenia, rich in nutrition, medical treatment, and health care. Known as "water ginseng" in the international market. [0003] Water shield is mainly distributed in China, Japan, Australia, North America and other places. In my country, it is mainly distributed in 7 provinces of Yunnan, Sichuan, Hunan, Hubei, Zhejiang and Jiangsu. Water shield in West Lake of Hangzhou is the most famous, which provides a reliable basis for the development of water shield products. The supply of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003C08B37/006
Inventor 冯思敏孙培龙栾迪孙雨欣李雨潼陈银宁丁喆夏玺越程浩
Owner ZHEJIANG UNIV OF TECH
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