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Application of a kind of colorimetric probe

A colorimetric probe and probe technology, applied in the field of probes, can solve problems such as poor water solubility, poor specificity, and complex structure, and achieve the effects of high selectivity, simple structure, and good water solubility

Active Publication Date: 2020-05-19
GUIZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the colorimetric probes reported in the literature have the disadvantages of poor water solubility, complex structure and poor specificity, and water solubility is crucial for the practical application of a probe, and few colorimetric probes can be realized in the water environment Identification and detection of hypochlorous acid

Method used

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  • Application of a kind of colorimetric probe
  • Application of a kind of colorimetric probe
  • Application of a kind of colorimetric probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The application of the colorimetric probe of the present embodiment, the molecular formula of the colorimetric probe is C 21 h 23 NO 4 S; the structural formula of the colorimetric probe is:

[0026]

[0027] The colorimetric probe is used to detect the total content of hypochlorous acid and hypochlorite in the aqueous solution, and the detection method is:

[0028] Step 1, dissolving the colorimetric probe in a phosphate buffer solution with a pH value of 5 at a concentration of 0.2mol / L to obtain a probe stock solution with a colorimetric probe of 0.5mmol / L, and then adding the probe stock solution to the probe stock solution Add 29 concentrations of 0mol / L to 1.4×10 -3 mol / L sodium hypochlorite aqueous solution, shake up and settle to 3mL with the phosphate buffer solution of 0.2mol / L pH value 5, obtain 29 standard solutions; The concentration of colorimetric probe in described 29 standard solutions is 0.1 mmol / L;

[0029] Step 2, measure the 29 standard solu...

Embodiment 2

[0035] This embodiment is the selectivity of the colorimetric probe of embodiment 1 to metal cations:

[0036] Dissolve the colorimetric probe in a phosphate buffer solution with a pH value of 5 at a concentration of 0.2 mol / L to obtain a probe stock solution, and then add ClO with a concentration of 0.4 mmol / L to the probe stock solution. - Aqueous solution, metal ion Li + 、Co 2+ 、Cr 3+ 、K + 、Cd 2+ , Pb 2+ , Ca 2+ , Hg 2+ 、Ba 2+ 、Cu 2+ , Mg 2+ 、Ni 2+ , Zn 2+ 、Al 3+ and Fe 3+ Shake up and dilute to 3mL with 0.2mol / L phosphate buffer solution with a pH value of 5 to obtain 16 sample solutions, the concentration of the colorimetric probe in the 16 sample solutions is 0.1mmol / L ;

[0037] Contrast: dissolving the colorimetric probe in a phosphate buffer solution with a pH value of 5 at a concentration of 0.2mol / L to obtain a control probe solution with a colorimetric probe concentration of 0.1mmol / L; in the control probe solution Does not contain ClO - or metal i...

Embodiment 3

[0042] This embodiment is the selectivity of the colorimetric probe of embodiment 1 to anions:

[0043] Dissolve the colorimetric probe in a phosphate buffer solution with a pH value of 5 at a concentration of 0.2mol / L to obtain a probe stock solution, and then add ClO with a concentration of 0.4mmol / L to the probe stock solution. - Aqueous solution, other anions AcO - , ClO 4 - , SO 4 2- 、CN - 、Br - , NO 2 - , CO 3 2- , I - and F - Shake up the aqueous solution and dilute to 3mL with 0.2mol / L phosphate buffer solution of pH 5 to obtain 10 sample solutions, the concentration of the colorimetric probe in the 10 sample solutions is 0.1mmol / L;

[0044] Contrast: dissolving the colorimetric probe in a phosphate buffer solution with a pH value of 5 at a concentration of 0.2mol / L to obtain a control probe solution with a colorimetric probe concentration of 0.1mmol / L; in the control probe solution Does not contain ClO - or other anions;

[0045] Sample solutions of 9 o...

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Abstract

The present invention provides an application of a colorimetric probe and is usded for detecting total content of hypochlorous acid and hypochlorite in an aqueous solution. The detection method comprises: preparing a probe stock solution, and separately adding different concentrations of sodium hypochlorite aqueous solutions to obtain a plurality of standard solutions; measuring an ultraviolet visible absorption light intensity value at 424 nm, and establishing a standard curve; adding an aqueous solution to be tested to the probe stock solution to obtain a sample solution; and measuring the ultraviolet visible absorption light intensity value of the sample solution at the 424 nm, and substituting into the standard curve to obtain the total content of hypochlorous acid and hypochlorite inthe aqueous solution to be tested. The colorimetric probe can instantly recognize the hypochlorous acid and the hypochlorite in the aqueous solution, and obtains the probe stock solution of which thecolor and the ultraviolet-visible absorption spectrum can change instantaneously, the colorimetric probe has specificity, high selectivity, has a linear relationship with the sodium hypochlorite witha concentration of 0mol / L to 2.8X10-4mol / L, has a large linear range, and can be used for accurate measurement of the hypochlorous acid and the hypochlorite in water environment.

Description

technical field [0001] The invention belongs to the technical field of probes, and in particular relates to the application of a colorimetric probe. Background technique [0002] Hypochlorous acid (HClO), as one of the important weakly acidic biological reactive oxygen species (ROS), is catalyzed by myeloperoxidase in leukocytes to produce endogenous hypochlorous acid (ClO 2 +H 2 O→HClO). Under certain physiological conditions, about half of hypochlorous acid (HClO) dissociates into hypochlorite (ClO - ). Endogenous hypochlorous acid widely exists in the living body, and exogenous hypochlorous acid is mainly produced by chlorination of water (Cl 2 +H 2 o 2 → HClO). Chlorination disinfection is widely used in the disinfection of drinking water such as tap water, well water and bottled water, public swimming pools and various departments of hospitals. Generally, its use concentration ranges from 10 -5 ~10 -2 mol / L. Under normal physiological conditions, a certain co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N21/31
CPCG01N21/31G01N21/78
Inventor 余青张奇龙徐红黄亚励吴翀
Owner GUIZHOU MEDICAL UNIV
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