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Method for producing whole-fat high-energy low-gossypol high-quality cottonseed kernels through bacterial enzyme collaborative glycolysis

A zymolysis and full-fat technology, which is applied in animal husbandry, food science, animal feed, etc., can solve problems such as use restrictions, and achieve the effects of promoting animal growth, inhibiting digestive tract diseases, and inhibiting growth and reproduction

Pending Publication Date: 2019-08-23
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These cottonseed meal containing gossypol can only be used as low-grade feed or fertilizer, and its use is severely limited

Method used

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  • Method for producing whole-fat high-energy low-gossypol high-quality cottonseed kernels through bacterial enzyme collaborative glycolysis
  • Method for producing whole-fat high-energy low-gossypol high-quality cottonseed kernels through bacterial enzyme collaborative glycolysis
  • Method for producing whole-fat high-energy low-gossypol high-quality cottonseed kernels through bacterial enzyme collaborative glycolysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for producing full-fat, high-energy, low-gossypol and high-quality cotton kernels through co-fermentation with bacteria and enzymes comprises the following steps:

[0021] 1) Heat treatment: After the cotton kernels are shelled and cake-pressed, add 40% water, heat-treat at 120°C for 25 minutes, dry at 50°C, and pulverize;

[0022] 2) Bacterial enzyme coordinated fermentation: inoculate 0.6% Bacillus subtilis seed liquid and 1.5% Saccharomyces cerevisiae seed liquid into cooled cotton kernels, add alkaline protease 50 u / g, mix well and place at 30°C 24 hours, during which the material was turned twice, and after the fermentation was completed, it was dried at 50°C to obtain full-fat, high-energy, low-gossypol and high-quality cotton kernels.

[0023] The preparation step of Bacillus subtilis seed solution is to inoculate the Bacillus subtilis bacillus subtilis whose preservation number is CCTCC NO: M 2017591 on the slant medium for activation, and then use the ...

Embodiment 2

[0026] A method for producing full-fat, high-energy, low-gossypol and high-quality cotton kernels through co-fermentation with bacteria and enzymes comprises the following steps:

[0027] 1) Heat treatment: After the cotton kernels are shelled and cake-pressed, add 45% water, heat-treat at 125°C for 22 minutes, dry at 45°C, and pulverize;

[0028] 2) Bacteria-enzyme coordinated fermentation: Inoculate 0.7% Bacillus subtilis seed liquid and 1.8% Saccharomyces cerevisiae seed liquid into cooled cotton kernels, add alkaline protease 60 u / g, mix well and place at 28°C 24 hours, during which the material was turned twice, and after the fermentation was completed, it was dried at 50°C to obtain full-fat, high-energy, low-gossypol and high-quality cotton kernels.

[0029] The preparation step of Bacillus subtilis seed solution is to inoculate the Bacillus subtilis bacillus subtilis with the preservation number of CCTCC NO: M 2017591 on the slant medium for activation, and then use th...

Embodiment 3

[0032] Get 1000kg cotton kernel, measure the content of its components such as crude protein, crude fat, crude fiber, free gossypol and amino acid, it is randomly divided into two parts, one part is used as the test group, and processed according to the following steps:

[0033] 1) Heat treatment: After the cotton kernels are shelled and cake-pressed, add 43% water, heat-treat at 130°C for 20 minutes, dry at 45°C, and pulverize;

[0034] 2) Bacteria and enzyme coordinated fermentation: inoculate 0.8% Bacillus subtilis seed liquid and 1.5% Saccharomyces cerevisiae seed liquid into cooled cotton kernels, add alkaline protease 60 u / g, mix well and place at 28°C 24 hours, during which the material was turned twice, and after the fermentation was completed, it was dried at 50°C to obtain full-fat, high-energy, low-gossypol and high-quality cotton kernels. Wherein, the preparation step of the Bacillus subtilis seed solution is to inoculate the Bacillus subtilis with the preservation n...

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Abstract

The invention discloses a method for producing whole-fat high-energy low-gossypol high-quality cottonseed kernels through bacterial enzyme collaborative glycolysis. Strains adopted in the bacterial enzyme collaborative glycolysis process are saccharomyces cerevisiae and bacillus subtilis. Physical, chemical and biological combined detoxification treatment is utilized to effectively remove gossypolin the cottonseed kernels, so that the content of free gossypol in the cottonseed kernels is 6000 mg / Kg or above and is reduced to 300 mg / Kg or below. The method has the effects that the prepared whole-fat high-energy low-gossypol high-quality cottonseed kernels can effectively inhibit the growth and reproduction of infectious microbes in the intestinal tract, the occurrence of digestive tract diseases is inhibited, the growth and propagation of beneficial bacteria are enhanced, the intestinal peristalsis is accelerated, and the growth of animals is promoted; the immunity of animal organismsis improved, the using quantity of drugs such as vaccines and antibiotics is reduced, the animal survival rate is improved, and meanwhile it is guaranteed that animal meat is green and healthy.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for producing full-fat, high-energy, low-gossypol, and high-quality cotton kernels through synergistic fermentation with bacteria and enzymes. Background technique [0002] my country has a large amount of cotton production, and the current annual output of cottonseed is 7.2 million tons, accounting for about 24% of the world's annual output. After oil extraction from these cottonseeds, 3.24 million tons of cottonseed meal can be obtained, equivalent to 140,000 tons of pure protein. In my country, there are 40 million tons of cake produced after oil production every year. After dephenolization, the protein content of cottonseed is more than 50%, which is higher than the protein content of 44% of first-grade soybean meal. Soybean meal is mostly used for feeding, and it is rich in cottonseed protein. Only a small amount of cotton meal is used for feeding, and 75%-80% of the co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/14A23L5/20
CPCA23K10/12A23K10/14A23L5/25A23L5/27
Inventor 孙宏吴逸飞汤江武李园成姚晓红王新沈琦
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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