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The SNP molecular marker and its application on the pig chromosome 7 related to the total number of teats

A technology of molecular markers and chromosomes, which is applied in the field of molecular biotechnology and molecular markers, can solve problems such as unsatisfactory results, and achieve the effects of increasing core competitiveness, accelerating the breeding process, and accelerating the progress of pig genetic improvement

Active Publication Date: 2020-10-09
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Researchers at home and abroad have used QTL mapping and candidate gene methods to study the quantitative trait loci (QTL) that affect the number of teats, but the results are not satisfactory

Method used

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  • The SNP molecular marker and its application on the pig chromosome 7 related to the total number of teats
  • The SNP molecular marker and its application on the pig chromosome 7 related to the total number of teats
  • The SNP molecular marker and its application on the pig chromosome 7 related to the total number of teats

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Experimental animals

[0035] The experimental pig group used in the present invention is 2153 purebred plus line Duroc of Wen's Food Group Co., Ltd. Breeding Pig Branch, which is the core group of Breeding Pig Branch.

[0036] In the resource group selected in this experiment, the Canadian Duroc breed pigs, the pigs have free access to food and water, and the entire feeding method and feeding conditions are always consistent, which is a conventional method.

[0037] (2) Sample collection

[0038] The above-mentioned piglet docked tail and ear tissues were collected and soaked in ethanol solution with a volume fraction of 75%, and stored in a -20°C refrigerator for later use.

[0039] (3) 50K SNP typing in the whole pig genome

[0040] The ear tissue or docked tail tissue collected from each individual of the 2153 Duroc breeding pigs selected from the above-mentioned resource groups, the whole genome DNA was extracted by the standard phenol-chloroform method, and ...

Embodiment 2

[0050] Example 2 Target DNA sequence amplification and sequencing

[0051] (1) Primer design

[0052] The DNA sequence of SEQ ID NO: 1 on pig chromosome 7 was downloaded from the Ensembl website (http: / / asia.ensembl.org / index.html). And use primer design software primer premier 6.0 to design primers. The DNA sequences of the designed primers are as follows:

[0053] P001-F: 5'-TTGTGGCGGAGGAGAAAGAGA-3';

[0054] P002-R: 5'-CGGACACCTGAAGTGCTGAT-3';

[0055] (2) PCR amplification

[0056] Add 1 μL of DNA template, 3.4 μL of double distilled water, 5 μL of 2×Tag PCR StanMix with Loading Dye, and 0.3 μL of primers P001-F and P002-R into a 10 μL reaction system. The PCR reaction conditions were as follows: 3 minutes of pre-denaturation at 95°C, 35 cycles of denaturation at 95°C for 30 s, annealing at 60°C for 30 s, extension at 72°C for 30 s, and finally 5 min at 72°C.

[0057] (3) DNA sequence determination

[0058] DNA sequence sequencing identification: carried out in Shen...

Embodiment 3

[0061] Example 3 SNP site g.131G>A effect analysis of molecular markers

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Abstract

The invention belongs to the technical field of a molecule biology technique and molecular markers, and particularly relates to an SNP molecular marker located on a porcine No. 7 chromosome and related to total teat number and an application of the SNP molecular marker. The SNP site of the SNP molecular marker located on a porcine No. 7 chromosome and related to total teat number corresponds to the 97568284th G>A mutation on the No. 7 chromosome of a reference sequence of an international porcine genome 11.1 edition. The invention also provides a primer pair of the SNP molecular marker locatedon a porcine No. 7 chromosome and related to total teat number. According to the SNP molecular marker disclosed by the invention, the dominant allele of the SNP is preferably selected, so that the dominant allele frequency can be increased by generation, the reproductive performance of pigs can be increased, the heredity improvement progress of the pigs can be accelerated, and economic benefits can be effectively increased.

Description

technical field [0001] The invention belongs to the technical fields of molecular biotechnology and molecular markers, and specifically relates to a SNP molecular marker located on pig No. 7 chromosome and related to the total teat number and its application. Background technique [0002] The sow's teat is an important organ for piglets to obtain nutrition and immunity during lactation. In recent years, selective breeding for increased litter size has increased the burden on sows to feed more piglets. Therefore, making pigs have a sufficient number of teats has always been an important goal of breeding pigs. The total teat number (TTN) is an important reproductive trait of pigs, and increasing the total teat number is an important means to improve piglet survival rate, promote piglet weight gain and uniform growth. Therefore, breeding for the trait of total teat number is very necessary to improve pig production performance. [0003] The reproductive performance of sows h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 吴珍芳杨杰叶勇庄站伟郑恩琴徐铮蔡更元
Owner SOUTH CHINA AGRI UNIV
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