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05SAR-PAGE and preparation method and application thereof

A technology of 05SAR-PAGE and formulation, applied in the field of 05SAR-PAGE and its preparation, can solve the problems of destruction and no enzyme activity analysis, etc., and achieve the effect of simple and convenient operation

Active Publication Date: 2019-08-16
WUHAN INST OF PHYSICS & MATHEMATICS CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Experiments have shown that 0.1% w / v SAR will destroy the structure of some conventional proteins, so SAR-PAGE containing 0.1% w / v SAR is not used for protein aggregation status and protein enzyme activity analysis

Method used

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  • 05SAR-PAGE and preparation method and application thereof
  • 05SAR-PAGE and preparation method and application thereof
  • 05SAR-PAGE and preparation method and application thereof

Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0059] Below in conjunction with accompanying drawing and embodiment describe in detail:

[0060] 1. 05SAR-PAGE

[0061] Table 1 prepares the solution used for Tris-glycine 6% 05SAR-PAGE polyacrylamide gel electrophoresis separation gel

[0062]

[0063] Table 2 prepares the solution used for Tris-glycine 8% 05SAR-PAGE polyacrylamide gel electrophoresis separation gel

[0064]

[0065] Table 3 prepares the solution used for Tris-glycine 10% 05SAR-PAGE polyacrylamide gel electrophoresis separation gel

[0066]

[0067]

[0068] Table 4 prepares the solution used for Tris-glycine 12% 05SAR-PAGE polyacrylamide gel electrophoresis separation gel

[0069]

[0070] Table 5 prepares the solution used for Tris-glycine 15% 05SAR-PAGE polyacrylamide gel electrophoresis separation gel

[0071]

[0072] Table 6 prepares the solution used for Tris-glycine 05SAR-PAGE polyacrylamide gel electrophoresis stacking gel

[0073]

[0074] 2. Application of 05SAR-PAGE

[0...

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Abstract

The invention discloses a 05SAR-PAGE and a preparation method and an application thereof, and relates to a gel electrophoresis separation technology. The 05SAR-PAGE is a gel made from 0.05%w / v of sodium lauroyl sarcosine. The gel comprises a lower separating gel and an upper stacking gel that are same in the length and thickness. The height ratio of the lower separating gel to the upper stacking gel is 2:1-8:1. The 05SAR-PAGE of the invention overcomes defects that the SDS-PAGE can not analyze protein modification in a physiological state and the Native-PAGE can not be calibrated with proteinMarker and can not be directly used for analyzing acidic protein and basic protein. The 05SAR-PAGE has universality, simple operation and low cost and can be widely used for fundamental and applied researches in the fields such as molecular dynamics, enzymology and medicine, and brings extensive convenience to the scientific analysis of protein by the majority of experimental personnel.

Description

technical field [0001] The invention relates to a gel electrophoresis separation technology, in particular to a 05SAR-PAGE and its preparation method and application. Background technique [0002] Protein is an important component of organisms and the main bearer of life activities. Proteins often exist in multimeric state, complex state or modified state to perform biological functions. At present, it is still challenging to accurately identify different states of proteins and protein interactions. [0003] SDS-PAGE and Native-PAGE are the most commonly used protein identification methods in the field of protein research. SDS is a strong anionic detergent, which can bind to proteins and break the structure of spherical proteins, so that the molecular weight of proteins is related to the amount of charge carried by proteins, so the migration speed of proteins during electrophoresis depends only on the molecular weight of proteins , and the protein marker can be used to ind...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447G01N33/68B01D57/02
CPCB01D57/02G01N27/447G01N33/6803
Inventor 黄丽群姜凌李从刚刘乙祥郑稳稳
Owner WUHAN INST OF PHYSICS & MATHEMATICS CHINESE ACADEMY OF SCI
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