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Method for adjusting prostate cancer tumor angiogenesis by utilizing miR-203/SNAI2 axis

A mir-203, psin-mir-203 technology, applied in the field of molecular detection, can solve the problem of no related records and reports

Inactive Publication Date: 2019-08-13
天津科美生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although Yi Qu et al. reported that the expression of miR-182 and miR-203 was repressed during the epithelial-mesenchymal transition (EMT) from normal prostate epithelial EP156T cells to progeny non-transformed EPT1 mesenchymal cells, whereas miR-182 and miR-203 Overexpression of miR-203 in normal prostate epithelial cells can induce mesenchymal-to-epithelial transition (MET) by regulating the expression of SNAI2, however, the miR-203 / SNAI2 axis has important effects on swollen prostate cancer cells, including cell proliferation, migration, angiogenesis , have key roles in stem cells, xenograft growth and related signaling pathways, etc., and there are no related records and reports

Method used

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  • Method for adjusting prostate cancer tumor angiogenesis by utilizing miR-203/SNAI2 axis

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Embodiment Construction

[0007] 1. Cell Culture

[0008] Five prostate cancer cell lines (DU145, PC3, 22Rv1, LNCaP, and C4-2) and two immortalized and untransformed prostate epithelial cell lines (PZ-HPV-7 and RWPE1) were obtained from ATCC, USA. Cells were cultured according to the standard manual of ATCC. Human umbilical vein endothelial cells (HuVECs) were purchased from Life Technologies (Carlsbad, California, USA) and cultured according to the instructions.

[0009] 2. Angiogenesis Experiment in HuVECs

[0010] Treated cells were seeded into 6-well plates. After growing to 90%, the cells were washed three times with PBS, and then cultured in 500 μl medium containing 1% serum for 24 h. The medium was then centrifuged at 1500 rpm, and the supernatant was collected. Total 5×10 4 HuVECs were seeded into each well of a 48-well plate with Matrigel lacking growth factors. After 8 h of culture, previously collected medium or HuVEC medium was added, capillary-like structures were captured using a mi...

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Abstract

The invention relates to the technical field of molecular detection, in particular to a method for detection of microRNAs and treatment of prostate cancers (PCa). The invention provides influence of an miR-203 / SNAI2 axis on in-vitro and in-vivo biological characteristics of the prostate cancers. Particularly, miR-203 is expressed in DU145 and PC3 cells to reduce angiogenesis, and the inhibitory effect of the miR-203 can be improved through the expression of the miR-203 and SNAI2. The miR-203 inhibits the formation of endothelial cells of prostate cancer cells and angiogenesis.

Description

technical field [0001] The invention relates to the technical field of molecular detection, in particular to a method for microRNA detection and treatment of prostate cancer (PCa). The present invention provides that the miR-203 / SNAI2 axis can be used as a diagnostic and therapeutic target for PCa. Background technique [0002] In recent years, the morbidity and mortality of prostate cancer (Prostate cancer, PCa) have risen sharply, and its growth rate ranks first among tumors. At the same time, the prevention and treatment of PCa is facing two major clinical problems, namely poor specificity of early diagnosis indicators and poor effect of late treatment methods. The occurrence and progression of prostate cancer is a multi-step process involving multiple gene mutations and abnormal gene regulation. Accumulating evidence indicates that dysregulation of specific genes, including microRNAs (miRNAs) and long non-coding RNAs (lncRNAs), is a major cause of the pathogenesis and ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886A61K31/7105A61K48/00A61P35/00
CPCA61K31/7105C12Q1/6886
Inventor 张志潜
Owner 天津科美生物技术有限公司
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