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A kind of sol preparation process and device for releasing dry bacteria from dormancy

A preparation process, the technology of dry bacteria, applied in the field of sol preparation, can solve the problems of low efficiency, long cycle of dry bacteria, low efficiency of breeding and cultivation of dry bacteria, etc., and achieve the effect of improving growth status and promoting repair

Active Publication Date: 2021-08-17
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, my country's utilization of forestry resources is showing an upward trend, and people's understanding of wild mushrooms in forests is gradually deepening, but it has always remained at a level that depends on the natural environment and gifts. In many studies, We can only study its habitat through basic science, and study its genetics through molecular biology. Taking dry bacteria as a typical example, due to the dormant period of dry bacteria, the formation of dry bacteria takes a long period, and people can only use inefficient methods to control them. Increasing propagules for cultivation, the breeding and cultivation efficiency of D. spp. is very low. At present, there is no public method of artificially interfering with D. spores to promote the germination of D. spores, which greatly limits the use of D. spores as a precious resource. use

Method used

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  • A kind of sol preparation process and device for releasing dry bacteria from dormancy
  • A kind of sol preparation process and device for releasing dry bacteria from dormancy
  • A kind of sol preparation process and device for releasing dry bacteria from dormancy

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Step 1: Use 400k, 250kpa high temperature and high pressure to kill the microorganisms in the natural rosin; then sterilize the preparation device, feed steam into the closed sterilization for three hours, adopt segmental sterilization during the sterilization process, close each outlet valve, The steam flow direction is regulated by the three-way air-throttle valve to form a loop for sterilization, the regulating valve on the pipeline of the diaphragm pump 18 is opened, and the air valve of the component mixing tank 20 is opened at the same time, and closed after sterilization;

[0037] Step 2: extract in advance according to the mass ratio of rosin: absolute ethanol = 120:900 to obtain a brown-yellow absolute ethanol rosin solution, and filter to remove the insoluble long-chain resin and pine needle weeds in the absolute ethanol rosin solution;

[0038] Step 3: Open the constant pressure pipeline valve j25 of the liquid delivery equipment, turn on the clean water pump ...

Embodiment 2

[0042] Step 1: use 410k, 251kpa high temperature and high pressure to kill the microorganisms in the natural rosin; then sterilize the preparation device, feed steam into the closed sterilization for three hours, adopt segmental sterilization during the sterilization process, close each outlet valve, The steam flow direction is regulated by the three-way air-throttle valve to form a loop for sterilization, the regulating valve on the pipeline of the diaphragm pump 18 is opened, and the air valve of the component mixing tank 20 is opened at the same time, and closed after sterilization;

[0043] Step 2: extract in advance according to the mass ratio of rosin: absolute ethanol = 135:920 to obtain a brown-yellow absolute ethanol rosin solution, and filter to remove the insoluble long-chain resin and pine needle weeds in the absolute ethanol rosin solution;

[0044]Step 3: Open the constant pressure pipeline valve j25 of the liquid delivery equipment, turn on the clean water pump 3...

Embodiment 3

[0048] Step 1: use 425k, 252kpa high temperature and high pressure to kill the microorganisms in the natural rosin; then sterilize the preparation device, feed steam into the closed sterilization for three hours, adopt segmental sterilization during the sterilization process, close each outlet valve, The steam flow direction is regulated by the three-way air-throttle valve to form a loop for sterilization, the regulating valve on the pipeline of the diaphragm pump 18 is opened, and the air valve of the component mixing tank 20 is opened at the same time, and closed after sterilization;

[0049] Step 2: extract in advance according to the mass ratio of rosin: absolute ethanol = 144:950 to obtain a brown-yellow absolute ethanol rosin solution, and filter to remove the insoluble long-chain resin and pine needle weeds in the absolute ethanol rosin solution;

[0050] Step 3: Open the constant pressure pipeline valve j25 of the liquid delivery equipment, turn on the clean water pump ...

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Abstract

The invention discloses a preparation process and a device for a sol that can release the dormant dry fungus fruit body and enter the growth phase. Rosin solution, mix the obtained anhydrous ethanol rosin solution with sterile water, prepare a sol by centrifugal spraying, add calcium nitrate and xylose to the prepared sol to obtain a sol that can dissolve dry bacteria; the preparation device includes: The liquid storage tank, the absolute ethanol storage tank and the Nobes steam generator adopt the staged sterilization method, which has good air tightness. The sol can promote the dormancy of dry bacteria, which provides a favorable foundation for the scientific research and propagation promotion technology of dry bacteria. The further expansion of the propagules of the dry bacterium can improve the woodland biome and promote the restoration of the damaged woodland.

Description

technical field [0001] The invention relates to the technical field of sol preparation, in particular to a sol preparation process and device for decomposing dried bacteria into dormancy. Background technique [0002] At present, my country's utilization of forestry resources is showing an upward trend, and people's understanding of wild fungi in forests is gradually deepening, but it always stays at a level that depends on the natural environment and gifts. In many studies, We can only study its habitat through basic science, and study its genetics through molecular biology. Taking dry bacteria as a typical example, due to the dormant period of dry bacteria, the formation of dry bacteria takes a long period, and people can only use inefficient methods to control them. Increasing propagules for cultivation, the breeding and cultivation efficiency of D. spp. is very low. At present, there is no public method of artificially interfering with D. spores to promote the germination ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J13/00A01G18/00
CPCA01G18/00B01J13/0004
Inventor 常相娜李跃童高畅蒋文茜赵磊屈腾飞
Owner SHAANXI UNIV OF SCI & TECH
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