Reagent kit and method for quickly detecting staphylococcus MecA

Abstract

The invention discloses a reagent kit and method for quickly detecting staphylococcus MecA. The reagent kit comprises cell lysate, amplifying reaction liquid, colloidal gold detection liquid and an immunochromatography reagent strip. The reagent kit is characterized in that transcription products of MecA and 16S rRNA are obtained through NASBA amplification on a primer pair in the amplifying reaction liquid. The reagent kit for quickly detecting genes of the staphylococcus MecA is stable in detection result, high in accuracy rate, high in sensitivity, low in cost and short in time consumptionand suitable for clinical promotion. The detection method is suitable for clinically and quickly detecting methicillin resistant staphylococcus and screening staphylococcus at pharynx nasalis, is quick and accurate, is simple in equipment, is low in requirements for operators, and has potential in bedside detection and application in hospitals at different levels.

Description

technical field [0001] The invention relates to the field of medical biology, in particular to a kit and method for rapidly detecting Staphylococcus MecA. Background technique [0002] Staphylococci, including Staphylococcus aureus (S. aureus) and Staphylococcus epidermidis (S. epidermidis), are widely distributed in the human population and in the natural environment. They are normal colonizers on the skin and mucous membranes of humans and animals and can be obtained from Soil, water, milk, food, etc. (Miragaia 2018) (Huijbers et al. 2015). Although staphylococci only exist as colonizing bacteria in most cases and do not cause harm to the body, when the host is immunocompromised or the skin and mucous membrane barriers are damaged, their invasion potential will appear, resulting in Hospital or community-acquired infection (Natsis and Cohen 2018; Poulakou et al. 2019). [0003] Antibiotic resistance is a threat to the prevention and treatment of bacterial infections. (Mi...

AI Technical Summary

Problems solved by technology

Several DNA amplification-based real-time fluorescent quantitative PCR techniques have been used to detect the MecA gene of Staphylococcus sp. (Chung et al.2016; Nijhuis et al.2014; Tang et al.2014), but these methods require complicated DNA extraction process, special PCR amplification conditions, specialized laboratories and thermostats (Chung et al.2016; Nijhuis et al.2014; Tang et al.2014), these tedious processes even accompanied by high costs cannot meet the needs of bed requirements for side-by-side detection and critical value reporting, thus limiting their widespread use

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