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Thylakoid lipid solid supporting membrane and construction method thereof

A construction method and supporting membrane technology, applied in chemical instruments and methods, membrane technology, semi-permeable membrane separation, etc., can solve the problems of not being able to simulate the microenvironment well, and achieve the effect of improving in vitro activity

Active Publication Date: 2019-07-16
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the environment constructed by these methods is far from the in vivo transmembrane microenvironment of PSII, and cannot simulate its microenvironment well.

Method used

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  • Thylakoid lipid solid supporting membrane and construction method thereof
  • Thylakoid lipid solid supporting membrane and construction method thereof
  • Thylakoid lipid solid supporting membrane and construction method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Modification of the substrate surface by chitosan

[0039] A gold-plated silicon wafer was selected as the substrate, and the piranha solution (piranha solution, H 2 SO 4 :H 2 o 2 =3:1 (v / v)) this substrate is cleaned, after that, the chitosan aqueous solution that concentration is 8mg / ml is added dropwise on the substrate surface that cleans, room temperature hatches 40min, forms the gold of chitosan modification. Surface base.

[0040] 2. Preparation of PSII-integrated thylakoid lipid vesicles

[0041] (1) Dissolve monogalactosyl diglyceride (MGDG), digalactosyl diglyceride (DGDG), thioisorhamnose diglyceride (SQDG) and phosphatidylglyceride (PG) in chloroform according to Close to its ratio in thylakoids and mix in brown vials (MGDG 50%, DGDG 31%, SQDG 8.3%, PG 10.7%), for the experimental group that needs to add cholesterol, at this time add as total thylakoid lipids 5% cholesterol by mole;

[0042] (2) Nitrogen gas is slowly introduced into the bottle, an...

Embodiment 2

[0054] 1. Modification of the substrate surface by chitosan

[0055] A gold-plated silicon wafer was selected as the substrate, and the piranha solution (piranha solution, H 2 SO 4 :H 2 o 2 =3:1 (v / v)) to clean the substrate, after that, the chitosan aqueous solution with a concentration of 10mg / ml is added dropwise on the cleaned substrate surface, and incubated at room temperature for 20min to form chitosan-modified gold Surface base.

[0056] 2. Preparation of PSII-integrated thylakoid lipid vesicles

[0057] (1) Dissolve monogalactosyl diglyceride (MGDG), digalactosyl diglyceride (DGDG), thioisorhamnose diglyceride (SQDG) and phosphatidylglyceride (PG) in chloroform according to Close to its ratio in thylakoids and mix in brown vials (MGDG 48%, DGDG 33%, SQDG 7.3%, PG 11.7%), for the experimental group that needs to add cholesterol, add as total thylakoid lipids at the same time 3% cholesterol by mole;

[0058] (2) Nitrogen gas is slowly introduced into the bottle, ...

Embodiment 3

[0069] 1. Modification of the substrate surface by chitosan

[0070] A gold-plated silicon wafer was selected as the substrate, and the piranha solution (piranha solution, H 2 SO 4 :H 2 o 2 =3:1 (v / v)) to clean the substrate, after that, the chitosan aqueous solution with a concentration of 15mg / ml is added dropwise on the cleaned substrate surface, and incubated at room temperature for 10min to form chitosan-modified gold Surface base.

[0071] 2. Preparation of PSII-integrated thylakoid lipid vesicles

[0072] (1) Dissolve monogalactosyl diglyceride (MGDG), digalactosyl diglyceride (DGDG), thioisorhamnose diglyceride (SQDG) and phosphatidylglyceride (PG) in chloroform according to Close to its ratio in thylakoids, mix them in brown vials (MGDG 52%, DGDG 29%, SQDG 9.3%, PG 9.7%). For the experimental group that needs to add cholesterol, add as total thylakoid lipids at the same time. 8% cholesterol by mole;

[0073] (2) Nitrogen gas is slowly introduced into the bottle...

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Abstract

The invention discloses a thylakoid lipid solid supporting membrane and a construction method thereof. The construction method comprises the following steps: providing a substrate, and modifying the surface of the substrate by hydrophilic polymer molecules to form a hydrophilic polymer molecule modified substrate; providing a thylakoid lipid vesicle, and integrating a photosystem II (PSII) into the thylakoid lipid vesicle to form a thylakoid lipid vesicle integrated with the PSII; and when the content of cholesterol in the thylakoid lipid vesicle is 3-8% mol and the concentration of Ca<2+> ina solution is 15-25 mM, hatching the thylakoid lipid vesicle integrated with the PSII on the surface of the hydrophilic polymer molecule modified substrate to obtain the thylakoid lipid solid supporting membrane integrated with the PSII. Through the method for constructing the thylakoid lipid vesicle solid supporting membrane provided by the invention, in-vitro activity of the PSII can be obviously improved.

Description

technical field [0001] The invention relates to a method for constructing a thylakoid lipid solid support membrane, in particular to a thylakoid lipid solid support membrane and a construction method thereof, and belongs to the technical field of bionic membrane preparation. Background technique [0002] PSII (Photosystem II) is a photosynthesis-related enzyme, mainly present on the thylakoid membrane in plant leaf cells, and is a transmembrane protein. PSII has the characteristics of splitting water molecules under light conditions, generating oxygen and protons, and transferring the released electrons to plastoquinone. Based on this unique property of PSII, it has important potential significance in the research of clean energy field. For example, the combination of PSII with inorganic catalysts is used to generate hydrogen; PSII-based photoelectrochemical cells are used to generate photocurrent. In these existing reports, researchers generally improve the stability of P...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D67/00B01D69/10C02F1/44
CPCB01D67/0002B01D69/10C02F1/44
Inventor 黄雷郝王平陈艳艳戴建武
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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