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Test tube potato production method

A production method and test-tube tuber technology, which is applied in the field of agricultural reproduction, can solve the problems of low test-tube tuber tuber formation rate, etc., and achieve the effects of short induced production time, increased utilization rate, and increased annual output

Active Publication Date: 2019-07-16
HEBEI AGRICULTURAL UNIV.
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention proposes a method for producing test-tube tubers of potatoes, which solves the problem of low fruiting rate of test-tube tubers of potatoes in the prior art

Method used

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Effect test

Embodiment 1

[0029] A method for producing test-tube tubers of potatoes, comprising the following steps:

[0030] SA. Medium production:

[0031] SA1.MS medium: solid MS medium powder purchased on the market, configure 1L according to the instructions for use, adjust the pH value to 5.8 with 0.1mol / L HCl or 0.1mol / L NaOH, shake well and distribute to tissue culture bottle, 50mL per bottle;

[0032] SA2. Strong seedling medium: Add 0.02mg / L PP333 to the MS medium solution, dilute to 1L, adjust the pH value to 5.8 with 0.1mol / L HCl or 0.1mol / L NaOH, shake well and distribute To tissue culture bottles, 50mL per bottle;

[0033] SA3. Tuber tuber induction medium: add 50g sucrose to the strong seedling medium solution, dilute to 1L, adjust the pH value to 5.8 with 0.1mol / L HCl or 0.1mol / L NaOH, add 0.5mL fungicide mother solution , shake well and then pack into tissue culture bottles, each bottle is 50mL; wherein the fungicide mother solution is composed of the following components by volume...

Embodiment 2

[0040] A method for producing test-tube tubers of potatoes, comprising the following steps:

[0041] SA. Medium production:

[0042] SA1.MS medium: solid MS medium powder purchased on the market, configure 1L according to the instructions for use, adjust the pH value to 5.8 with 0.1mol / L HCl or 0.1mol / L NaOH, shake well and distribute to tissue culture bottle, 50mL per bottle;

[0043] SA2. Strong seedling medium: Add 0.02mg / L PP333 to the MS medium solution, dilute to 1L, adjust the pH value to 5.8 with 0.1mol / L HCl or 0.1mol / L NaOH, shake well and distribute To tissue culture bottles, 50mL per bottle;

[0044] SA3. Tuber tuber induction medium: add 50g sucrose to the strong seedling medium solution, dilute to 1L, adjust the pH value to 5.8 with 0.1mol / L HCl or 0.1mol / L NaOH, add 0.5mL fungicide mother solution , shake well and then pack into tissue culture bottles, each bottle is 50mL; wherein the fungicide mother solution is composed of the following components by volume...

Embodiment 3

[0051] A method for producing test-tube tubers of potatoes, comprising the following steps:

[0052] SA. Medium production:

[0053] SA1.MS medium: solid MS medium powder purchased on the market, configure 1L according to the instructions for use, adjust the pH value to 5.8 with 0.1mol / L HCl or 0.1mol / L NaOH, shake well and distribute to tissue culture bottle, 50mL per bottle;

[0054] SA2. Strong seedling medium: Add 0.02mg / L PP333 to the MS medium solution, dilute to 1L, adjust the pH value to 5.8 with 0.1mol / L HCl or 0.1mol / L NaOH, shake well and distribute To tissue culture bottles, 50mL per bottle;

[0055] SA3. Tuber tuber induction medium: add 50g sucrose to the strong seedling medium solution, dilute to 1L, adjust the pH value to 5.8 with 0.1mol / L HCl or 0.1mol / L NaOH, add 0.5mL fungicide mother solution , shake well and then pack into tissue culture bottles, each bottle is 50mL; wherein the fungicide mother solution is composed of the following components by volume...

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Abstract

The invention belongs to the technical field of agricultural propagation and provides a test tube potato production method. The method includes following steps: S0, propagating virus-free seedlings: culturing the virus-free seedlings on an MS culture medium for 3 weeks to obtain basic seedlings; S1, culturing strong seedlings: cutting the basic seedlings by one axillary bud for each stem node layer by layer, and transferring into a strong seedling culture medium for culturing for 25-30d to obtain strong seedlings, wherein the strong seedling culture medium is composed of MS+PP333 0.02mg / L, andHCl 0.1mol / L or NaOH 0.1mol / L is adopted to adjust pH to 5.8; S2, performing induced culture: cutting the strong seedlings obtained in the S1 by one axillary bud for each stem node layer by layer, and transferring into a test tube potato induced culture medium for culturing for 18-25d to obtain induced seedlings, wherein the test tube potato induced culture medium is composed of MS+PP333 0.02mg / L+sucrose 50g / L, and HCl 0.1mol / L or NaOH 0.1mol / L is adopted to adjust pH to 5.8; S3, inducing test tube potato: inducing the induced seedlings obtained in the S2 in a constant-temperature culture boxfor 28-60d to obtain test tube potato. By the technical scheme, the problem that test tube potato is low in tuberization rate in the prior art is solved.

Description

technical field [0001] The invention belongs to the technical field of agricultural propagation methods, and relates to a method for producing test-tube potatoes. Background technique [0002] The test tube tuber is induced by the virus-free seedlings in the culture bottle, and the tubers with a general diameter of 2-10mm are formed by the swelling of the axillary buds. It has the advantage that the virus-free seedlings do not carry the main potato viruses and other pathogens that cause seed potato degeneration. At the same time, the test-tube tubers are easier to cultivate and manage than the test-tube seedlings, and have a high survival rate; the small size is convenient for storage, communication and transportation; production is not affected by the climate. Impact, can be annual factory production. [0003] At present, the relatively advanced production technology of test-tube tubers is the achievement of Professor Xie Conghua of Huazhong Agricultural University. He use...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 崔江慧薛薇高玉坤任根增项晓冬韩芳王建民
Owner HEBEI AGRICULTURAL UNIV.
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