Test tube potato production method

A production method and test-tube tuber technology, which is applied in the field of agricultural reproduction, can solve the problems of low test-tube tuber tuber formation rate, etc., and achieve the effects of short induced production time, increased utilization rate, and increased annual output

Active Publication Date: 2019-07-16
HEBEI AGRICULTURAL UNIV.
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention proposes a method for producing test-tube tubers of potatoes, which solves the problem of low fruiting rate of test-tube tubers of potatoes in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Test tube potato production method
  • Test tube potato production method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for producing test-tube tubers of potatoes, comprising the following steps:

[0030] SA. Medium production:

[0031] SA1.MS medium: solid MS medium powder purchased on the market, configure 1L according to the instructions for use, adjust the pH value to 5.8 with 0.1mol / L HCl or 0.1mol / L NaOH, shake well and distribute to tissue culture bottle, 50mL per bottle;

[0032] SA2. Strong seedling medium: Add 0.02mg / L PP333 to the MS medium solution, dilute to 1L, adjust the pH value to 5.8 with 0.1mol / L HCl or 0.1mol / L NaOH, shake well and distribute To tissue culture bottles, 50mL per bottle;

[0033] SA3. Tuber tuber induction medium: add 50g sucrose to the strong seedling medium solution, dilute to 1L, adjust the pH value to 5.8 with 0.1mol / L HCl or 0.1mol / L NaOH, add 0.5mL fungicide mother solution , shake well and then pack into tissue culture bottles, each bottle is 50mL; wherein the fungicide mother solution is composed of the following components by volume...

Embodiment 2

[0040] A method for producing test-tube tubers of potatoes, comprising the following steps:

[0041] SA. Medium production:

[0042] SA1.MS medium: solid MS medium powder purchased on the market, configure 1L according to the instructions for use, adjust the pH value to 5.8 with 0.1mol / L HCl or 0.1mol / L NaOH, shake well and distribute to tissue culture bottle, 50mL per bottle;

[0043] SA2. Strong seedling medium: Add 0.02mg / L PP333 to the MS medium solution, dilute to 1L, adjust the pH value to 5.8 with 0.1mol / L HCl or 0.1mol / L NaOH, shake well and distribute To tissue culture bottles, 50mL per bottle;

[0044] SA3. Tuber tuber induction medium: add 50g sucrose to the strong seedling medium solution, dilute to 1L, adjust the pH value to 5.8 with 0.1mol / L HCl or 0.1mol / L NaOH, add 0.5mL fungicide mother solution , shake well and then pack into tissue culture bottles, each bottle is 50mL; wherein the fungicide mother solution is composed of the following components by volume...

Embodiment 3

[0051] A method for producing test-tube tubers of potatoes, comprising the following steps:

[0052] SA. Medium production:

[0053] SA1.MS medium: solid MS medium powder purchased on the market, configure 1L according to the instructions for use, adjust the pH value to 5.8 with 0.1mol / L HCl or 0.1mol / L NaOH, shake well and distribute to tissue culture bottle, 50mL per bottle;

[0054] SA2. Strong seedling medium: Add 0.02mg / L PP333 to the MS medium solution, dilute to 1L, adjust the pH value to 5.8 with 0.1mol / L HCl or 0.1mol / L NaOH, shake well and distribute To tissue culture bottles, 50mL per bottle;

[0055] SA3. Tuber tuber induction medium: add 50g sucrose to the strong seedling medium solution, dilute to 1L, adjust the pH value to 5.8 with 0.1mol / L HCl or 0.1mol / L NaOH, add 0.5mL fungicide mother solution , shake well and then pack into tissue culture bottles, each bottle is 50mL; wherein the fungicide mother solution is composed of the following components by volume...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of agricultural propagation and provides a test tube potato production method. The method includes following steps: S0, propagating virus-free seedlings: culturing the virus-free seedlings on an MS culture medium for 3 weeks to obtain basic seedlings; S1, culturing strong seedlings: cutting the basic seedlings by one axillary bud for each stem node layer by layer, and transferring into a strong seedling culture medium for culturing for 25-30d to obtain strong seedlings, wherein the strong seedling culture medium is composed of MS+PP333 0.02mg / L, andHCl 0.1mol / L or NaOH 0.1mol / L is adopted to adjust pH to 5.8; S2, performing induced culture: cutting the strong seedlings obtained in the S1 by one axillary bud for each stem node layer by layer, and transferring into a test tube potato induced culture medium for culturing for 18-25d to obtain induced seedlings, wherein the test tube potato induced culture medium is composed of MS+PP333 0.02mg / L+sucrose 50g / L, and HCl 0.1mol / L or NaOH 0.1mol / L is adopted to adjust pH to 5.8; S3, inducing test tube potato: inducing the induced seedlings obtained in the S2 in a constant-temperature culture boxfor 28-60d to obtain test tube potato. By the technical scheme, the problem that test tube potato is low in tuberization rate in the prior art is solved.

Description

technical field [0001] The invention belongs to the technical field of agricultural propagation methods, and relates to a method for producing test-tube potatoes. Background technique [0002] The test tube tuber is induced by the virus-free seedlings in the culture bottle, and the tubers with a general diameter of 2-10mm are formed by the swelling of the axillary buds. It has the advantage that the virus-free seedlings do not carry the main potato viruses and other pathogens that cause seed potato degeneration. At the same time, the test-tube tubers are easier to cultivate and manage than the test-tube seedlings, and have a high survival rate; the small size is convenient for storage, communication and transportation; production is not affected by the climate. Impact, can be annual factory production. [0003] At present, the relatively advanced production technology of test-tube tubers is the achievement of Professor Xie Conghua of Huazhong Agricultural University. He use...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 崔江慧薛薇高玉坤任根增项晓冬韩芳王建民
Owner HEBEI AGRICULTURAL UNIV.
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap