Experimental device and experimental method for establishing blast injury cell model

Abstract

The invention relates to an experimental device and an experimental method for establishing a blast injury cell model. The experimental device comprises a high-pressure gas cylinder, a pressure reducing valve, a connecting pipe, a driving section, an electromagnetic valve, a driven section, a pressure sensor and a cell culture dish bracket, and the high-pressure gas cylinder, the pressure reducingvalve, the connecting pipe, the driving section, the electromagnetic valve, the driven section, the pressure sensor and the cell culture dish bracket are connected in sequence, wherein the cell culture dish bracket comprises an external thread interface and an internal thread cap, and an annular bulge is arranged in the external thread interface. The invention also discloses an experimental method of the experimental device. Compared with the prior art, the experimental device is simple to manufacture, safe and stable, and can accurately control the pressure ratio between the driving sectionand the driven section by using the electromagnetic valve to form stable and repeatable shock waves, so that an accurate, controllable and repeatable blast injury cell model can be established.

Description

technical field [0001] The invention relates to the technical field of cell model construction, in particular to an experimental device and an experimental method for establishing a blast injury cell model. Background technique [0002] Blast injury is the damage to the body, especially air-containing organs such as the lungs, hearing apparatus, and gastrointestinal tract, caused by the overpressure and negative pressure of the shock wave. With the outbreak of local modern wars, frequent terrorist activities and serious unexpected industrial accidents, the research on the injury mechanism and treatment technology of impact injuries has attracted more and more attention. Establishing a scientific and accurate model is an important experimental method and means to realize impact injury research. At present, scholars at home and abroad have designed and developed a variety of impact injury animal models, which are mainly divided into the following two categories according to th...

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Problems solved by technology

TNT explosion source has the complexity and high risk of transportation, storage and detonation, and the experimental model has batch effect and poor repeatability; 2. Shock tube

The animal model of shock tube shock injury is relatively mature and widely used, but it also has the disadvantages of large size, complicated operation and high cost

However, the response of the body after shock injury is a very complex pathophysiological process, and various factors can affect the occurrence and development of the injury. At this time, it is impossible to accurately simulate the damage caused by the shock wave to the body by using the shock tube shock injury animal model. To study and analyze the cascade reaction between cells and reveal the pathophysiological changes of tissue cells after impact injury, so it is necessary to study and explore the injury mechanism of impact injury from the molecular level

However, there is currently no experimental device specially used to establish a cell model of blast injury. In order to better study the mechanism and pathophysiological changes of blast injury and provide a reliable basis for the treatment of blast injury, a standard, accurate, and controllable model should be established. The impact injury cell model is of great significance

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